Click here to switch to the map view.

The map label for this gene is hemF

Identifier: 157371701

GI number: 157371701

Start: 3833904

End: 3834824

Strand: Direct

Name: hemF

Synonym: Spro_3464

Alternate gene names: 157371701

Gene position: 3833904-3834824 (Clockwise)

Preceding gene: 157371700

Following gene: 157371702

Centisome position: 70.36

GC content: 60.59

Gene sequence:

>921_bases
ATGAGTTTATCGACCATCGCAGAAGTAAAAACCTTCCTGCTGGCGCTGCAAGACCGCATCTGCACGCAGTTGGCCCAGGC
CGACGGCAGCGCCGTTTTCAACGAAGACCGCTGGACGCGTGAAGAAGGCGGCGGTGGCCGCAGCCGGGTGTTGACCGAGG
GTGCGGTGTTCGAACAGGCGGGCGTCAACTTCTCACACGTCTCCGGCGCTACCCTGCCCGCTTCCGCTACCGCGCATCGC
CCCGAACTGGCCGGACGCAGTTTCCAGGCGATGGGCGTGTCGCTGGTGATCCATCCGCGCAGCCCTTACATTCCGACCAG
CCACGCCAACGTGCGCTTTTTCATTGCGGAAAAGCCGGGCGAAGAGCCGGTGTGGTGGTTTGGCGGCGGCTTTGACCTGA
CGCCGTTTTATGGCTTTGAAGAAGATGCCGTTCACTGGCACCGCACCGCCGAACGGCTGTGTGCGCCCTTCGGTGAGCAG
GTCTATCCCAAATACAAAAAGTGGTGCGACGACTATTTCTTTATCAAACACCGTAACGAAGCACGCGGCATCGGCGGCCT
GTTCTTTGATGACCTGAACACCCCGGACTTCGCCACCTGCTTCGCGTTTACCCAGGCGGTGGGCGAAGGCTTCCTCGAGG
CCTATCTGCCGATCGTAGAGAAACGCAAAACGCTGAGCTGGGGCGATCACGAGCGCCAGTTCCAACTGTATCGTCGCGGC
CGCTACGTAGAATTCAATTTGGTCTGGGACCGGGGCACGCTGTTCGGCCTGCAAACCGGTGGCCGCACCGAATCCATCCT
GATGTCGATGCCGCCGCTGGTGCGTTGGGAATACAATTATCAACCGGCGGAAGACAGCCCGGAAGCGGCGCTGTACCGTG
ATTTCCTGCCGGTACGCGATTGGCTGAAGGAGCCGGAATGA

Upstream 100 bases:

>100_bases
TTACGATTCACACCAACGAAAACCCAGCTGATACTGCCAGCCGCGGTGAATAAAGGTATACTCTGCGCCTTGTTCAGTCA
GACGCTGTGAATTATCCCGT

Downstream 100 bases:

>100_bases
TGCAGATTTGGGTTGATGCGGACGCCTGTCCCAACGTAATTAAAGAAGTGCTGTTTCGGGCGGCGGAGCGTACCGCAATG
TCGGTGACGCTGGTGGCGAA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSLSTIAEVKTFLLALQDRICTQLAQADGSAVFNEDRWTREEGGGGRSRVLTEGAVFEQAGVNFSHVSGATLPASATAHR
PELAGRSFQAMGVSLVIHPRSPYIPTSHANVRFFIAEKPGEEPVWWFGGGFDLTPFYGFEEDAVHWHRTAERLCAPFGEQ
VYPKYKKWCDDYFFIKHRNEARGIGGLFFDDLNTPDFATCFAFTQAVGEGFLEAYLPIVEKRKTLSWGDHERQFQLYRRG
RYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYNYQPAEDSPEAALYRDFLPVRDWLKEPE

Sequences:

>Translated_306_residues
MSLSTIAEVKTFLLALQDRICTQLAQADGSAVFNEDRWTREEGGGGRSRVLTEGAVFEQAGVNFSHVSGATLPASATAHR
PELAGRSFQAMGVSLVIHPRSPYIPTSHANVRFFIAEKPGEEPVWWFGGGFDLTPFYGFEEDAVHWHRTAERLCAPFGEQ
VYPKYKKWCDDYFFIKHRNEARGIGGLFFDDLNTPDFATCFAFTQAVGEGFLEAYLPIVEKRKTLSWGDHERQFQLYRRG
RYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYNYQPAEDSPEAALYRDFLPVRDWLKEPE
>Mature_305_residues
SLSTIAEVKTFLLALQDRICTQLAQADGSAVFNEDRWTREEGGGGRSRVLTEGAVFEQAGVNFSHVSGATLPASATAHRP
ELAGRSFQAMGVSLVIHPRSPYIPTSHANVRFFIAEKPGEEPVWWFGGGFDLTPFYGFEEDAVHWHRTAERLCAPFGEQV
YPKYKKWCDDYFFIKHRNEARGIGGLFFDDLNTPDFATCFAFTQAVGEGFLEAYLPIVEKRKTLSWGDHERQFQLYRRGR
YVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYNYQPAEDSPEAALYRDFLPVRDWLKEPE

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=304, Percent_Identity=49.0131578947368, Blast_Score=291, Evalue=6e-79,
Organism=Escherichia coli, GI1788777, Length=295, Percent_Identity=78.6440677966102, Blast_Score=493, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320249, Length=302, Percent_Identity=43.3774834437086, Blast_Score=245, Evalue=8e-66,
Organism=Drosophila melanogaster, GI17864388, Length=302, Percent_Identity=48.0132450331126, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_SERP5 (A8GHH2)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479690.1
- STRING:   A8GHH2
- GeneID:   5604766
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3464
- eggNOG:   COG0408
- HOGENOM:   HBG631180
- OMA:   HMNTRFV
- ProtClustDB:   PRK05330
- BioCyc:   SPRO399741:SPRO_3464-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 34887; Mature: 34755

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 108-108 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSTIAEVKTFLLALQDRICTQLAQADGSAVFNEDRWTREEGGGGRSRVLTEGAVFEQA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHEEECCCHHHHC
GVNFSHVSGATLPASATAHRPELAGRSFQAMGVSLVIHPRSPYIPTSHANVRFFIAEKPG
CCCEEECCCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEECCCC
EEPVWWFGGGFDLTPFYGFEEDAVHWHRTAERLCAPFGEQVYPKYKKWCDDYFFIKHRNE
CCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHCCHHHHHHCCCEEEEEECCC
ARGIGGLFFDDLNTPDFATCFAFTQAVGEGFLEAYLPIVEKRKTLSWGDHERQFQLYRRG
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
RYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYNYQPAEDSPEAALYRDFLPVRD
CEEEEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
WLKEPE
HHCCCC
>Mature Secondary Structure 
SLSTIAEVKTFLLALQDRICTQLAQADGSAVFNEDRWTREEGGGGRSRVLTEGAVFEQA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHEEECCCHHHHC
GVNFSHVSGATLPASATAHRPELAGRSFQAMGVSLVIHPRSPYIPTSHANVRFFIAEKPG
CCCEEECCCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEECCCC
EEPVWWFGGGFDLTPFYGFEEDAVHWHRTAERLCAPFGEQVYPKYKKWCDDYFFIKHRNE
CCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHCCHHHHHHCCCEEEEEECCC
ARGIGGLFFDDLNTPDFATCFAFTQAVGEGFLEAYLPIVEKRKTLSWGDHERQFQLYRRG
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
RYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYNYQPAEDSPEAALYRDFLPVRD
CEEEEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
WLKEPE
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA