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The map label for this gene is amiA [H]

Identifier: 157371700

GI number: 157371700

Start: 3832935

End: 3833834

Strand: Direct

Name: amiA [H]

Synonym: Spro_3463

Alternate gene names: 157371700

Gene position: 3832935-3833834 (Clockwise)

Preceding gene: 157371698

Following gene: 157371701

Centisome position: 70.34

GC content: 57.78

Gene sequence:

>900_bases
ATGAAAAAGCAGGTTAAACAATTCCTCAATTTTAAGCCGCTCTCCGTTTCCCCCACGCGACGTCAATTACTGCTCTCCGG
CCTGGCGTTGGTGCTGCTCGGCAGCAAAAGCCAAAGCGCCGAAGCCGAGTCCGGTGGCATGCTGCGCAACCAACATCCCA
AACCGACCGCTAAACCAGCGCCTAAACCCTCCGGCAGCAAAAGGCTGGTGATGATCGACCCTGGCCATGGCGGTATCGAC
TCCGGCGCGGTTGGCAGTGAAGGCTCACAGGAAAAGCACGTCGTGTTGGAGATTGCCAACCACGTCCGCCGCATCCTGCA
CGAACAGGAACACGTCGAAGTCCGTCTGACACGGGAAGAAGACGAATTTATCCCGCTGTTCCAACGGGTGGAGATTGCCC
ACCAGCATCAGGCCGATCTGTTTATCTCGATTCACGCCGACGGTTTTACCAGCCCGGAAGCCTCAGGCGCTTCGGTGTTT
GCCCTGTCCAATCGCGGGGCCAGCAGCGCCATGGCGCGTTACCTGTCCAACCGTGAAAACGCCGCCGATGATGTGGCCGG
TGGCAAGTACAAAAACCAGGACAACTATCTGCAACAGGTGCTGTTTGATCTGGTGCAGACCGATACCATCAACAACAGCC
TGACGCTCGGCCGCCACGTGCTCGGCCAAATCCGGCCGGTACACCACCTACACAGCAACAGTACGGAACAGGCGGCATTT
GCGGTGCTGAAATCCCCGTCTATCCCGTCGGTGCTGGTCGAAACCTCGTTCATTACCAACCCGGCAGAAGAGCGTCTGCT
GGGCACCACCGCATTCCGCGAGAAAATCGCCCGCGCCATTGCCGACGGGGTGATCAGTTTCTTCAGCTATTACGATTCAC
ACCAACGAAAACCCAGCTGA

Upstream 100 bases:

>100_bases
GGTTACCCGCGTAATAAATGGACATAGTTATCAGGTCGATTAATGATTATCAATACGATATAACGTCGCAAAATAGAATT
TGTCGACGGAGCGTAGCGTT

Downstream 100 bases:

>100_bases
TACTGCCAGCCGCGGTGAATAAAGGTATACTCTGCGCCTTGTTCAGTCAGACGCTGTGAATTATCCCGTATGAGTTTATC
GACCATCGCAGAAGTAAAAA

Product: N-acetylmuramoyl-l-alanine amidase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKKQVKQFLNFKPLSVSPTRRQLLLSGLALVLLGSKSQSAEAESGGMLRNQHPKPTAKPAPKPSGSKRLVMIDPGHGGID
SGAVGSEGSQEKHVVLEIANHVRRILHEQEHVEVRLTREEDEFIPLFQRVEIAHQHQADLFISIHADGFTSPEASGASVF
ALSNRGASSAMARYLSNRENAADDVAGGKYKNQDNYLQQVLFDLVQTDTINNSLTLGRHVLGQIRPVHHLHSNSTEQAAF
AVLKSPSIPSVLVETSFITNPAEERLLGTTAFREKIARAIADGVISFFSYYDSHQRKPS

Sequences:

>Translated_299_residues
MKKQVKQFLNFKPLSVSPTRRQLLLSGLALVLLGSKSQSAEAESGGMLRNQHPKPTAKPAPKPSGSKRLVMIDPGHGGID
SGAVGSEGSQEKHVVLEIANHVRRILHEQEHVEVRLTREEDEFIPLFQRVEIAHQHQADLFISIHADGFTSPEASGASVF
ALSNRGASSAMARYLSNRENAADDVAGGKYKNQDNYLQQVLFDLVQTDTINNSLTLGRHVLGQIRPVHHLHSNSTEQAAF
AVLKSPSIPSVLVETSFITNPAEERLLGTTAFREKIARAIADGVISFFSYYDSHQRKPS
>Mature_299_residues
MKKQVKQFLNFKPLSVSPTRRQLLLSGLALVLLGSKSQSAEAESGGMLRNQHPKPTAKPAPKPSGSKRLVMIDPGHGGID
SGAVGSEGSQEKHVVLEIANHVRRILHEQEHVEVRLTREEDEFIPLFQRVEIAHQHQADLFISIHADGFTSPEASGASVF
ALSNRGASSAMARYLSNRENAADDVAGGKYKNQDNYLQQVLFDLVQTDTINNSLTLGRHVLGQIRPVHHLHSNSTEQAAF
AVLKSPSIPSVLVETSFITNPAEERLLGTTAFREKIARAIADGVISFFSYYDSHQRKPS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=286, Percent_Identity=65.034965034965, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI87082163, Length=246, Percent_Identity=47.9674796747967, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1790611, Length=249, Percent_Identity=36.144578313253, Blast_Score=161, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 32798; Mature: 32798

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQVKQFLNFKPLSVSPTRRQLLLSGLALVLLGSKSQSAEAESGGMLRNQHPKPTAKPA
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PKPSGSKRLVMIDPGHGGIDSGAVGSEGSQEKHVVLEIANHVRRILHEQEHVEVRLTREE
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
DEFIPLFQRVEIAHQHQADLFISIHADGFTSPEASGASVFALSNRGASSAMARYLSNREN
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
AADDVAGGKYKNQDNYLQQVLFDLVQTDTINNSLTLGRHVLGQIRPVHHLHSNSTEQAAF
CHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
AVLKSPSIPSVLVETSFITNPAEERLLGTTAFREKIARAIADGVISFFSYYDSHQRKPS
HHHCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKQVKQFLNFKPLSVSPTRRQLLLSGLALVLLGSKSQSAEAESGGMLRNQHPKPTAKPA
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PKPSGSKRLVMIDPGHGGIDSGAVGSEGSQEKHVVLEIANHVRRILHEQEHVEVRLTREE
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
DEFIPLFQRVEIAHQHQADLFISIHADGFTSPEASGASVFALSNRGASSAMARYLSNREN
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
AADDVAGGKYKNQDNYLQQVLFDLVQTDTINNSLTLGRHVLGQIRPVHHLHSNSTEQAAF
CHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
AVLKSPSIPSVLVETSFITNPAEERLLGTTAFREKIARAIADGVISFFSYYDSHQRKPS
HHHCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]