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The map label for this gene is holE [C]

Identifier: 157371450

GI number: 157371450

Start: 3538926

End: 3539117

Strand: Direct

Name: holE [C]

Synonym: Spro_3213

Alternate gene names: 157371450

Gene position: 3538926-3539117 (Clockwise)

Preceding gene: 157371448

Following gene: 157371451

Centisome position: 64.95

GC content: 47.92

Gene sequence:

>192_bases
ATGGGTCGAAAGCTGGATAGCTTACCGCAAGCAGAACGTGAAAAAATTGAAGTCGATTTACTGGCCCTCAGCGTCATTTA
CAACGAACGCTATGGCATTACCGTCAATGACGCCAGCAATGCCGAACAACAGGTGCCCGATTATTTACGCCCCTACTTTC
ATTTACGCCTGAACTACTATCGCGGTGCGTAA

Upstream 100 bases:

>100_bases
CAGGCACAGTCGCCCACATCTTGAGGCTCCCCCCAGAATGGGGTAGGGACAAGCGCGGCTAACGATCCTATGATGCCCCA
GGAATCACAGGGAGAATAAT

Downstream 100 bases:

>100_bases
TACAGCGCATTTCGGTGATTAACACTAACGACCGCTTGGCATCGTTCATCTCGCCGTTACAATAGGGGCATATTCCCTAC
GCACGCGAGAGATGCGATTT

Product: hypothetical protein

Products: Pyrophosphate; DNA [C]

Alternate protein names: NA

Number of amino acids: Translated: 63; Mature: 62

Protein sequence:

>63_residues
MGRKLDSLPQAEREKIEVDLLALSVIYNERYGITVNDASNAEQQVPDYLRPYFHLRLNYYRGA

Sequences:

>Translated_63_residues
MGRKLDSLPQAEREKIEVDLLALSVIYNERYGITVNDASNAEQQVPDYLRPYFHLRLNYYRGA
>Mature_62_residues
GRKLDSLPQAEREKIEVDLLALSVIYNERYGITVNDASNAEQQVPDYLRPYFHLRLNYYRGA

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Exact Function Of The Theta Subunit Is Unknown. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 7374; Mature: 7243

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKLDSLPQAEREKIEVDLLALSVIYNERYGITVNDASNAEQQVPDYLRPYFHLRLNYY
CCCCCCCCCCCCHHHHHHHHHEEEEEECCCCCEEECCCCCCHHCCHHHCCCEEEEEEEEE
RGA
CCC
>Mature Secondary Structure 
GRKLDSLPQAEREKIEVDLLALSVIYNERYGITVNDASNAEQQVPDYLRPYFHLRLNYY
CCCCCCCCCCCHHHHHHHHHEEEEEECCCCCEEECCCCCCHHCCHHHCCCEEEEEEEEE
RGA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: Deoxynucleoside Triphosphate [C]

Specific reaction: (n)Deoxynucleoside Triphosphate = (n)Pyrophosphate + DNA(n) [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10 -Phenanthroline; 2', 3'-Dideoxythymidine 5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA