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The map label for this gene is argT [H]

Identifier: 157371444

GI number: 157371444

Start: 3533029

End: 3533820

Strand: Direct

Name: argT [H]

Synonym: Spro_3207

Alternate gene names: 157371444

Gene position: 3533029-3533820 (Clockwise)

Preceding gene: 157371443

Following gene: 157371448

Centisome position: 64.84

GC content: 55.18

Gene sequence:

>792_bases
ATGAAAACAAAAAGTAGCCTGGTTTTATTGCTGCCACTGGCATTCAGTTTCGCCGCCCTTGGCGGGGAGTTCAGCGGCAA
GGTGATTAAGTTGGGCATAGACCCCACCTTCCCGCCGCTGGAATACAAAACTGCGCAAGGAACCCTGACCGGGTTCGGTG
TGGATATCGCTCAGGCGCTGTGCAATGAGATGCAGGCCAGGTGCGTGTGGGTCGAAAGCAGTTGGGACGGGATGATCCCG
GGGTTGCAGGCAAAAAAGTTTGACGCCATCGCCTCGTCAATGACCATTACGCCGCAGCGCGAAAAGCAGATCTCCTTCTC
GAACAAGGTGTCTAACGCCCCGGCACGCCTGGTGGCCCGCAAGGGTAGCGACCTGCAGCCCACGGCGGCTTCACTGAAAG
GCAAATCAATTGGCGTGCAACAAGGCTCCAGCCAGGAAGCTTACGCAAACGCAATGTGGCGCCAGGCCGGCGTCAACGTG
ATTGCCTATCAGAGCCAACAGGAAGTGAATGAGGATCTGGCCAACGGCCGGTTGGACGCTTCGCTGCTGGCCAGCGTCAG
CGCCAGCGAATTTTTCAAAACGCCCGGCGGCAAGGATTTTGCCTTTACCGGCGCGGAACTGAGCGACAGCAAATATTTTG
GTATTGGCGATGGCATTGGCGTGCGTAAAGAGGACCGCGCATTACTTGATGCCTTCAACGCGGCGTTGAAAGCCATTATC
GCCAACGGCACCTATAAGAAGGTGAACGATAAATACTTTGATTTTGATATGTACGGTAAGGGTAGTAACTGA

Upstream 100 bases:

>100_bases
CGACGAGTGTTTGCCGATTGGCGCCACTTACTGGGTCAAGCTGGCGGAACGCTTCCTGACTTAATCCCTTTTTGAACACC
ACTACGCTGAGGAAAACCAA

Downstream 100 bases:

>100_bases
GGGGCAGATTAAAAGGGCCAGGCGAGCGCCTGGCCCGATAAAAACGGTTACTTCACTTCGCCCATAGCCTTCAGCTTTTT
GGACAGTTCGCGACGTTCTT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKTKSSLVLLLPLAFSFAALGGEFSGKVIKLGIDPTFPPLEYKTAQGTLTGFGVDIAQALCNEMQARCVWVESSWDGMIP
GLQAKKFDAIASSMTITPQREKQISFSNKVSNAPARLVARKGSDLQPTAASLKGKSIGVQQGSSQEAYANAMWRQAGVNV
IAYQSQQEVNEDLANGRLDASLLASVSASEFFKTPGGKDFAFTGAELSDSKYFGIGDGIGVRKEDRALLDAFNAALKAII
ANGTYKKVNDKYFDFDMYGKGSN

Sequences:

>Translated_263_residues
MKTKSSLVLLLPLAFSFAALGGEFSGKVIKLGIDPTFPPLEYKTAQGTLTGFGVDIAQALCNEMQARCVWVESSWDGMIP
GLQAKKFDAIASSMTITPQREKQISFSNKVSNAPARLVARKGSDLQPTAASLKGKSIGVQQGSSQEAYANAMWRQAGVNV
IAYQSQQEVNEDLANGRLDASLLASVSASEFFKTPGGKDFAFTGAELSDSKYFGIGDGIGVRKEDRALLDAFNAALKAII
ANGTYKKVNDKYFDFDMYGKGSN
>Mature_263_residues
MKTKSSLVLLLPLAFSFAALGGEFSGKVIKLGIDPTFPPLEYKTAQGTLTGFGVDIAQALCNEMQARCVWVESSWDGMIP
GLQAKKFDAIASSMTITPQREKQISFSNKVSNAPARLVARKGSDLQPTAASLKGKSIGVQQGSSQEAYANAMWRQAGVNV
IAYQSQQEVNEDLANGRLDASLLASVSASEFFKTPGGKDFAFTGAELSDSKYFGIGDGIGVRKEDRALLDAFNAALKAII
ANGTYKKVNDKYFDFDMYGKGSN

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=50.5836575875486, Blast_Score=260, Evalue=5e-71,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=47.8448275862069, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1787085, Length=248, Percent_Identity=38.3064516129032, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=32.4, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=30.4347826086957, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=28.6885245901639, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28182; Mature: 28182

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKSSLVLLLPLAFSFAALGGEFSGKVIKLGIDPTFPPLEYKTAQGTLTGFGVDIAQAL
CCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEECCCCCEEECCHHHHHHH
CNEMQARCVWVESSWDGMIPGLQAKKFDAIASSMTITPQREKQISFSNKVSNAPARLVAR
HHHHCEEEEEEECCCCCCCCCCCHHHHHHHHHCEECCCCHHHHCCHHHHCCCCCHHHHCC
KGSDLQPTAASLKGKSIGVQQGSSQEAYANAMWRQAGVNVIAYQSQQEVNEDLANGRLDA
CCCCCCCHHHHCCCCEECCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCH
SLLASVSASEFFKTPGGKDFAFTGAELSDSKYFGIGDGIGVRKEDRALLDAFNAALKAII
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
ANGTYKKVNDKYFDFDMYGKGSN
CCCCCEECCCEEEEEEECCCCCC
>Mature Secondary Structure
MKTKSSLVLLLPLAFSFAALGGEFSGKVIKLGIDPTFPPLEYKTAQGTLTGFGVDIAQAL
CCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEECCCCCEEECCHHHHHHH
CNEMQARCVWVESSWDGMIPGLQAKKFDAIASSMTITPQREKQISFSNKVSNAPARLVAR
HHHHCEEEEEEECCCCCCCCCCCHHHHHHHHHCEECCCCHHHHCCHHHHCCCCCHHHHCC
KGSDLQPTAASLKGKSIGVQQGSSQEAYANAMWRQAGVNVIAYQSQQEVNEDLANGRLDA
CCCCCCCHHHHCCCCEECCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCH
SLLASVSASEFFKTPGGKDFAFTGAELSDSKYFGIGDGIGVRKEDRALLDAFNAALKAII
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
ANGTYKKVNDKYFDFDMYGKGSN
CCCCCEECCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]