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The map label for this gene is hipO [H]

Identifier: 157371443

GI number: 157371443

Start: 3531823

End: 3532992

Strand: Direct

Name: hipO [H]

Synonym: Spro_3206

Alternate gene names: 157371443

Gene position: 3531823-3532992 (Clockwise)

Preceding gene: 157371441

Following gene: 157371444

Centisome position: 64.82

GC content: 58.55

Gene sequence:

>1170_bases
ATGAGCAATCCGCTTGTTTTACCTGAAATAGCTGCCGTTCAACACGAAATGGTCGCCATTCGTCACCATATTCATGCGCA
TCCTGAACTGGGGTTCAATGAGTTTGCCACCAGCGATTTGGTGGCAAAACTGCTGTCCGAATGGGGATATCAGGTCACGC
GCCATATAGGACAAACCGGGGTTGTCGCCACGCTGCAAAGCGGCGTGGGGAAAACGCTTGGCCTGCGAGCCGATATGGAT
GCATTGCCGATTGAAGAAACCAGCGGACTGCCCTATGCCAGTACCCACAGCGGCGTGATGCACGCCTGCGGTCATGATGG
CCACACTACCATGCTGCTGGCGGCGGCGCGTTATCTGGCGCACCACTCTGACTTTACCGGTACATTGCGGTTAATATTTC
AGCCGGCGGAAGAAGGCGGCGGCGGCGCCCGGGTAATGATGGAAGACGGGCTGTTCGAGCGTTTTCCCTGTGACGCGGTG
TTCGCCATGCACAACGTGCCCGGCCTCCCGGTCGGCCAACTGGGCTTTGCCAGCGGCCCTTTCATGTGTTCGGCCGATAC
CGTCAACATCACCCTGCATGGGCACGGCGGGCACGGCGCGGTGCCACAGCACACCGTCGATCCGGTGGTGGTCTGTGCCG
CCATCGTGATGAGCCTGCAGACCATCGTCTCCAGAAATATCGATCCACAGGAAACGGCGATTGTGACCGTGGGCGCGATC
CAGGCCGGTCATGCCAGCAATGTCATTCCCTCGACCGCCACCATGACGCTGAGCGTGCGTGCGCTGAATGCCGATGTCCG
TCAACGGTTGGAAGACCGCATTACGGCACTGGTGGAAGCGCAGGCCGTCAGTTTTGGCGCCCGGGCAGAGATTGATTATC
AACAGGGTTATCCGGTGCTGGTGAACCACGCGGCGGAAACCGAACTGGCGCGTTCGGTCGCGTTGGAGTGGGCCGGTGAA
AGCCGGGTTATCGCCAATCTGCGCCCGATTACCGCCAGTGAGGACTTCGCTTTTATGCTGGAAAAATGCCCCGGCAGTTA
TATCTCAATTGGCAATGGTGAAAGCACGCCCGGGAATTCGCTGCATAACGCCGGTTACGATTTTAACGACGAGTGTTTGC
CGATTGGCGCCACTTACTGGGTCAAGCTGGCGGAACGCTTCCTGACTTAA

Upstream 100 bases:

>100_bases
CGGCGGCTCCCCGGGCAAAAGGTTGTTTGTTAGTCTCCGAAGTACCTTGTTCCCGGTTATCGATCGCGTCGGGAACCGAA
AATTCACTTTGGATATCGCG

Downstream 100 bases:

>100_bases
TCCCTTTTTGAACACCACTACGCTGAGGAAAACCAAATGAAAACAAAAAGTAGCCTGGTTTTATTGCTGCCACTGGCATT
CAGTTTCGCCGCCCTTGGCG

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSNPLVLPEIAAVQHEMVAIRHHIHAHPELGFNEFATSDLVAKLLSEWGYQVTRHIGQTGVVATLQSGVGKTLGLRADMD
ALPIEETSGLPYASTHSGVMHACGHDGHTTMLLAAARYLAHHSDFTGTLRLIFQPAEEGGGGARVMMEDGLFERFPCDAV
FAMHNVPGLPVGQLGFASGPFMCSADTVNITLHGHGGHGAVPQHTVDPVVVCAAIVMSLQTIVSRNIDPQETAIVTVGAI
QAGHASNVIPSTATMTLSVRALNADVRQRLEDRITALVEAQAVSFGARAEIDYQQGYPVLVNHAAETELARSVALEWAGE
SRVIANLRPITASEDFAFMLEKCPGSYISIGNGESTPGNSLHNAGYDFNDECLPIGATYWVKLAERFLT

Sequences:

>Translated_389_residues
MSNPLVLPEIAAVQHEMVAIRHHIHAHPELGFNEFATSDLVAKLLSEWGYQVTRHIGQTGVVATLQSGVGKTLGLRADMD
ALPIEETSGLPYASTHSGVMHACGHDGHTTMLLAAARYLAHHSDFTGTLRLIFQPAEEGGGGARVMMEDGLFERFPCDAV
FAMHNVPGLPVGQLGFASGPFMCSADTVNITLHGHGGHGAVPQHTVDPVVVCAAIVMSLQTIVSRNIDPQETAIVTVGAI
QAGHASNVIPSTATMTLSVRALNADVRQRLEDRITALVEAQAVSFGARAEIDYQQGYPVLVNHAAETELARSVALEWAGE
SRVIANLRPITASEDFAFMLEKCPGSYISIGNGESTPGNSLHNAGYDFNDECLPIGATYWVKLAERFLT
>Mature_388_residues
SNPLVLPEIAAVQHEMVAIRHHIHAHPELGFNEFATSDLVAKLLSEWGYQVTRHIGQTGVVATLQSGVGKTLGLRADMDA
LPIEETSGLPYASTHSGVMHACGHDGHTTMLLAAARYLAHHSDFTGTLRLIFQPAEEGGGGARVMMEDGLFERFPCDAVF
AMHNVPGLPVGQLGFASGPFMCSADTVNITLHGHGGHGAVPQHTVDPVVVCAAIVMSLQTIVSRNIDPQETAIVTVGAIQ
AGHASNVIPSTATMTLSVRALNADVRQRLEDRITALVEAQAVSFGARAEIDYQQGYPVLVNHAAETELARSVALEWAGES
RVIANLRPITASEDFAFMLEKCPGSYISIGNGESTPGNSLHNAGYDFNDECLPIGATYWVKLAERFLT

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=421, Percent_Identity=28.2660332541568, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41518; Mature: 41386

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPLVLPEIAAVQHEMVAIRHHIHAHPELGFNEFATSDLVAKLLSEWGYQVTRHIGQTG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVATLQSGVGKTLGLRADMDALPIEETSGLPYASTHSGVMHACGHDGHTTMLLAAARYLA
HHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
HHSDFTGTLRLIFQPAEEGGGGARVMMEDGLFERFPCDAVFAMHNVPGLPVGQLGFASGP
HCCCCCEEEEEEEEECCCCCCCEEEEEECCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCC
FMCSADTVNITLHGHGGHGAVPQHTVDPVVVCAAIVMSLQTIVSRNIDPQETAIVTVGAI
EEEECCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
QAGHASNVIPSTATMTLSVRALNADVRQRLEDRITALVEAQAVSFGARAEIDYQQGYPVL
ECCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEE
VNHAAETELARSVALEWAGESRVIANLRPITASEDFAFMLEKCPGSYISIGNGESTPGNS
EECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCC
LHNAGYDFNDECLPIGATYWVKLAERFLT
CCCCCCCCCCCEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
SNPLVLPEIAAVQHEMVAIRHHIHAHPELGFNEFATSDLVAKLLSEWGYQVTRHIGQTG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVATLQSGVGKTLGLRADMDALPIEETSGLPYASTHSGVMHACGHDGHTTMLLAAARYLA
HHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
HHSDFTGTLRLIFQPAEEGGGGARVMMEDGLFERFPCDAVFAMHNVPGLPVGQLGFASGP
HCCCCCEEEEEEEEECCCCCCCEEEEEECCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCC
FMCSADTVNITLHGHGGHGAVPQHTVDPVVVCAAIVMSLQTIVSRNIDPQETAIVTVGAI
EEEECCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
QAGHASNVIPSTATMTLSVRALNADVRQRLEDRITALVEAQAVSFGARAEIDYQQGYPVL
ECCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEE
VNHAAETELARSVALEWAGESRVIANLRPITASEDFAFMLEKCPGSYISIGNGESTPGNS
EECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCC
LHNAGYDFNDECLPIGATYWVKLAERFLT
CCCCCCCCCCCEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]