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The map label for this gene is purR [H]

Identifier: 157371434

GI number: 157371434

Start: 3521303

End: 3522334

Strand: Direct

Name: purR [H]

Synonym: Spro_3197

Alternate gene names: 157371434

Gene position: 3521303-3522334 (Clockwise)

Preceding gene: 157371429

Following gene: 157371435

Centisome position: 64.62

GC content: 58.14

Gene sequence:

>1032_bases
ATGATGGCGAAGAAACCCGGCGCGATCCCCGCGCCCACTGCACGCAAAGCGACGGCCAGTGATGTGGCGGCCCGCGCCGG
CGTGTCCAAATGGACGGTATCGCGTGCCTTTACCGACGGTGCGTCGATCTCACCCCATGCGAGGTTGCGCGTTCAAACCG
CGGCCAGAGAATTGGGTTATCGCCCTAATCTGCTGGCGCGCAGCCTGTCGAAAAAAAGCACCCGCATCATCGGGCTGGTG
GCGGACGAGCTAAAAAACCCGCATATTTTCACGCTGCTGGACGAAGTCACCCGGCAGTTGCAAAGCCGCGGCTATATGGC
CTTGCTGCTGAATATCACCTCGGAACACGACTATGAATCGGTGCTGACGCTGGCCGATCAAATGCAGGTGGATGGCCTGC
TGTTTCTCGGCACCCTGCTTAACGATCGGCTGATCACGCTGGCACAGGATATTCACCATATTCCACTGGTGGTGCTGTAT
CGCTATAGCGAAAGCCCCTATATCCAGGTGTTGGCCACCAACGGCTATCAGGCCGGGCGTGAAATTGGCGAACTGTTGCT
GACGCAAAATTATCAGCGCATGGGCTATCTGGCTGGGCCAGTGAGTGAATCCACCCAATTACGCCGCCTTGACGGTTTCC
GCGCCGCGCTGGCGGAAAAGCAACGCGACGTCAGCGTGGTGCTGCAGGCCCCGCATTATCAACGTCAGTGCGGCATGGAA
GCCTTCGCGGCCTACCTGGCAACCACCCCGGCGGAACAACGGGTTGAGGCGATTTTTTGTGAGAACGATATTCTGGCAGT
GGGGGTTATCGACGCCATCCGCGCCACACCGGGCTGTGCGCCGATTGCCGTGGTGGGGTTTGATGACATTGAACTGGCCG
CCTCCCCCAGTTACCAGTTGACCACTTATCGCCAGCCGATGCAGTCGCTGATTGCCGATGGCATTCATTGCCTGACGCAG
GCTTTCGAACCGGGCGGCCAACGTTTATATAACGGTGAATTAATCGTCCGGCGGTCGCATATTAAACAATAA

Upstream 100 bases:

>100_bases
TCCTGCACCAGGGATCCCACCGCTGACGGCAGCAACTCCCGCATCCGGCAAGGCTGTGCTAATCTGTTGAACCCATACCG
TTTTTTCATCACGAGCAAAG

Downstream 100 bases:

>100_bases
TGATATCAACAGACGGCGAGAACTCGGGTCAGGCTTATTGAGGTCATATGTCAGCTATCTATGATTGGTCGGTGTTGGCA
TTACGCCGTATTTACGATCG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MMAKKPGAIPAPTARKATASDVAARAGVSKWTVSRAFTDGASISPHARLRVQTAARELGYRPNLLARSLSKKSTRIIGLV
ADELKNPHIFTLLDEVTRQLQSRGYMALLLNITSEHDYESVLTLADQMQVDGLLFLGTLLNDRLITLAQDIHHIPLVVLY
RYSESPYIQVLATNGYQAGREIGELLLTQNYQRMGYLAGPVSESTQLRRLDGFRAALAEKQRDVSVVLQAPHYQRQCGME
AFAAYLATTPAEQRVEAIFCENDILAVGVIDAIRATPGCAPIAVVGFDDIELAASPSYQLTTYRQPMQSLIADGIHCLTQ
AFEPGGQRLYNGELIVRRSHIKQ

Sequences:

>Translated_343_residues
MMAKKPGAIPAPTARKATASDVAARAGVSKWTVSRAFTDGASISPHARLRVQTAARELGYRPNLLARSLSKKSTRIIGLV
ADELKNPHIFTLLDEVTRQLQSRGYMALLLNITSEHDYESVLTLADQMQVDGLLFLGTLLNDRLITLAQDIHHIPLVVLY
RYSESPYIQVLATNGYQAGREIGELLLTQNYQRMGYLAGPVSESTQLRRLDGFRAALAEKQRDVSVVLQAPHYQRQCGME
AFAAYLATTPAEQRVEAIFCENDILAVGVIDAIRATPGCAPIAVVGFDDIELAASPSYQLTTYRQPMQSLIADGIHCLTQ
AFEPGGQRLYNGELIVRRSHIKQ
>Mature_343_residues
MMAKKPGAIPAPTARKATASDVAARAGVSKWTVSRAFTDGASISPHARLRVQTAARELGYRPNLLARSLSKKSTRIIGLV
ADELKNPHIFTLLDEVTRQLQSRGYMALLLNITSEHDYESVLTLADQMQVDGLLFLGTLLNDRLITLAQDIHHIPLVVLY
RYSESPYIQVLATNGYQAGREIGELLLTQNYQRMGYLAGPVSESTQLRRLDGFRAALAEKQRDVSVVLQAPHYQRQCGME
AFAAYLATTPAEQRVEAIFCENDILAVGVIDAIRATPGCAPIAVVGFDDIELAASPSYQLTTYRQPMQSLIADGIHCLTQ
AFEPGGQRLYNGELIVRRSHIKQ

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=300, Percent_Identity=28.6666666666667, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790194, Length=312, Percent_Identity=31.0897435897436, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=29.0419161676647, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790369, Length=336, Percent_Identity=26.4880952380952, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=27.5747508305648, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787906, Length=262, Percent_Identity=30.1526717557252, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789202, Length=303, Percent_Identity=27.0627062706271, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787580, Length=290, Percent_Identity=26.2068965517241, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=26.1127596439169, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994940, Length=298, Percent_Identity=23.8255033557047, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786268, Length=279, Percent_Identity=27.2401433691756, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37763; Mature: 37763

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKKPGAIPAPTARKATASDVAARAGVSKWTVSRAFTDGASISPHARLRVQTAARELGY
CCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHEEHHHHHHHCCC
RPNLLARSLSKKSTRIIGLVADELKNPHIFTLLDEVTRQLQSRGYMALLLNITSEHDYES
CCCHHHHHHHHCCCEEEEEEHHHHCCCEEEHHHHHHHHHHHHCCEEEEEEEECCCCCHHH
VLTLADQMQVDGLLFLGTLLNDRLITLAQDIHHIPLVVLYRYSESPYIQVLATNGYQAGR
HHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCHHHH
EIGELLLTQNYQRMGYLAGPVSESTQLRRLDGFRAALAEKQRDVSVVLQAPHYQRQCGME
HHHHHHHHCCHHHCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCHH
AFAAYLATTPAEQRVEAIFCENDILAVGVIDAIRATPGCAPIAVVGFDDIELAASPSYQL
HHHHHHHCCCHHHHHHEEEECCCEEEEEHHHHHHCCCCCCCEEEEECCCEEECCCCCCEE
TTYRQPMQSLIADGIHCLTQAFEPGGQRLYNGELIVRRSHIKQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCC
>Mature Secondary Structure
MMAKKPGAIPAPTARKATASDVAARAGVSKWTVSRAFTDGASISPHARLRVQTAARELGY
CCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHEEHHHHHHHCCC
RPNLLARSLSKKSTRIIGLVADELKNPHIFTLLDEVTRQLQSRGYMALLLNITSEHDYES
CCCHHHHHHHHCCCEEEEEEHHHHCCCEEEHHHHHHHHHHHHCCEEEEEEEECCCCCHHH
VLTLADQMQVDGLLFLGTLLNDRLITLAQDIHHIPLVVLYRYSESPYIQVLATNGYQAGR
HHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCHHHH
EIGELLLTQNYQRMGYLAGPVSESTQLRRLDGFRAALAEKQRDVSVVLQAPHYQRQCGME
HHHHHHHHCCHHHCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCHH
AFAAYLATTPAEQRVEAIFCENDILAVGVIDAIRATPGCAPIAVVGFDDIELAASPSYQL
HHHHHHHCCCHHHHHHEEEECCCEEEEEHHHHHHCCCCCCCEEEEECCCEEECCCCCCEE
TTYRQPMQSLIADGIHCLTQAFEPGGQRLYNGELIVRRSHIKQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA