Click here to switch to the map view.

The map label for this gene is chaA [H]

Identifier: 157371160

GI number: 157371160

Start: 3214446

End: 3215546

Strand: Direct

Name: chaA [H]

Synonym: Spro_2920

Alternate gene names: 157371160

Gene position: 3214446-3215546 (Clockwise)

Preceding gene: 157371159

Following gene: 157371162

Centisome position: 58.99

GC content: 57.95

Gene sequence:

>1101_bases
ATGAAGTCGCAAAACGATCCTGGCCGATCCAAATCCCGCCACACTGAGTATTCCCTGTTGCTCCCTATCGCCGCCCTGGT
GGTGCTGAATCTGTGGGGCACTACCAGCAACTTTCCGCTGATCGTCGGCATTAACATCGTTGCCTTGATCGGTATTCTCA
GCAGCGCGTTCAGCGTAGTGCGCCACGCCGACGTTCTGGCCCACCGCCTGGGTGAACCCTATGGCTCGCTGATCCTCAGC
CTGTCGGTGGTGATCCTGGAAGTCAGCCTGATTTCAGCGCTGATGGCCACCGGCGACGCCGCGCCGGCGCTGATGCGCGA
TACGCTTTACTCGATCATTATGATTGTCAGCGCGGGGCTGGTCGGCGTTGCGCTGCTGCTTGGCGGCCGTAAATTCGCTA
CCCAGTACGTAAACCTGGGCGGCATAAAACAGTATCTGATGGCGATTTTCCCACTGGCGGTGATTGTGCTGGTGTTCCCG
AGTGCGCTGCCCGGCGGTAACTTCACTACCGGTCAGGCACTGCTGGTTTCGGTGATATCTGCCGCCATGTACGGCGTGTT
CCTGGTGATCCAGACCAAAACTCACCAGAGCCTGTTCGTCTATGAGCACGAAGATGACGATGGCGATCCGCACCATGGCA
AGCCTTCCTCGCACAGCAGTGCCTGGCATGCTGTTTGGCTGATCGTGCATCTGGTTGCGGTGATCGCGGTAACCAAGTTT
AACGCCAATCCGCTGGAAGGTTTGTTGACCAAGGTAAATGCCCCGGCGCAATTTACCGGCTTCCTGGTCGCTCTGCTGAT
CCTGTCGCCTGAAGGTCTGGGGGCACTGCGTGCGGTATTGAATAATCAGGTACAGCGCGCCATGAACCTGTTCTTCGGCT
CGGTGCTGGCGACCATTTCCCTGACGGTACCGGCGGTCACCGTCATCGCCACGCTGACCGGTCAGACGTTGATCTTCGGT
TTGCAGACGCCACATATCGTGGTAATGCTGACGGTGTTGCTGCTGTGCCAGCTTTCGTTCTCCACCGGCAGAACCAACGT
GCTGAACGGGACTGCGCACCTGGCACTGTTCGCCGCCTATATGATGACTATCTTTGCCTGA

Upstream 100 bases:

>100_bases
TATTATTATTAAATATAATTACATTCTGGATGCGCAGAGTTGCCTTTAACGGCAGTCAGCGATGACGGGACCACGCTGTT
TGAGAAGGAGTACCATCAAA

Downstream 100 bases:

>100_bases
TTAACCGATGGGCGCCCTGTTGGCGCCCATCGCCTTTATAGCGCGAAAGTTTAAATCGCGAAGAAAATACCCGCCGCAAT
GCCACCAAACAAAATCCATT

Product: calcium/sodium:proton antiporter

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKSQNDPGRSKSRHTEYSLLLPIAALVVLNLWGTTSNFPLIVGINIVALIGILSSAFSVVRHADVLAHRLGEPYGSLILS
LSVVILEVSLISALMATGDAAPALMRDTLYSIIMIVSAGLVGVALLLGGRKFATQYVNLGGIKQYLMAIFPLAVIVLVFP
SALPGGNFTTGQALLVSVISAAMYGVFLVIQTKTHQSLFVYEHEDDDGDPHHGKPSSHSSAWHAVWLIVHLVAVIAVTKF
NANPLEGLLTKVNAPAQFTGFLVALLILSPEGLGALRAVLNNQVQRAMNLFFGSVLATISLTVPAVTVIATLTGQTLIFG
LQTPHIVVMLTVLLLCQLSFSTGRTNVLNGTAHLALFAAYMMTIFA

Sequences:

>Translated_366_residues
MKSQNDPGRSKSRHTEYSLLLPIAALVVLNLWGTTSNFPLIVGINIVALIGILSSAFSVVRHADVLAHRLGEPYGSLILS
LSVVILEVSLISALMATGDAAPALMRDTLYSIIMIVSAGLVGVALLLGGRKFATQYVNLGGIKQYLMAIFPLAVIVLVFP
SALPGGNFTTGQALLVSVISAAMYGVFLVIQTKTHQSLFVYEHEDDDGDPHHGKPSSHSSAWHAVWLIVHLVAVIAVTKF
NANPLEGLLTKVNAPAQFTGFLVALLILSPEGLGALRAVLNNQVQRAMNLFFGSVLATISLTVPAVTVIATLTGQTLIFG
LQTPHIVVMLTVLLLCQLSFSTGRTNVLNGTAHLALFAAYMMTIFA
>Mature_366_residues
MKSQNDPGRSKSRHTEYSLLLPIAALVVLNLWGTTSNFPLIVGINIVALIGILSSAFSVVRHADVLAHRLGEPYGSLILS
LSVVILEVSLISALMATGDAAPALMRDTLYSIIMIVSAGLVGVALLLGGRKFATQYVNLGGIKQYLMAIFPLAVIVLVFP
SALPGGNFTTGQALLVSVISAAMYGVFLVIQTKTHQSLFVYEHEDDDGDPHHGKPSSHSSAWHAVWLIVHLVAVIAVTKF
NANPLEGLLTKVNAPAQFTGFLVALLILSPEGLGALRAVLNNQVQRAMNLFFGSVLATISLTVPAVTVIATLTGQTLIFG
LQTPHIVVMLTVLLLCQLSFSTGRTNVLNGTAHLALFAAYMMTIFA

Specific function: According to PubMed:8496184 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=365, Percent_Identity=77.8082191780822, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 38887; Mature: 38887

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQNDPGRSKSRHTEYSLLLPIAALVVLNLWGTTSNFPLIVGINIVALIGILSSAFSVV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
RHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPALMRDTLYSIIMIVSAGL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VGVALLLGGRKFATQYVNLGGIKQYLMAIFPLAVIVLVFPSALPGGNFTTGQALLVSVIS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AAMYGVFLVIQTKTHQSLFVYEHEDDDGDPHHGKPSSHSSAWHAVWLIVHLVAVIAVTKF
HHHHHHEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NANPLEGLLTKVNAPAQFTGFLVALLILSPEGLGALRAVLNNQVQRAMNLFFGSVLATIS
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVPAVTVIATLTGQTLIFGLQTPHIVVMLTVLLLCQLSFSTGRTNVLNGTAHLALFAAY
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MMTIFA
HHHHCC
>Mature Secondary Structure
MKSQNDPGRSKSRHTEYSLLLPIAALVVLNLWGTTSNFPLIVGINIVALIGILSSAFSVV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
RHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPALMRDTLYSIIMIVSAGL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VGVALLLGGRKFATQYVNLGGIKQYLMAIFPLAVIVLVFPSALPGGNFTTGQALLVSVIS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AAMYGVFLVIQTKTHQSLFVYEHEDDDGDPHHGKPSSHSSAWHAVWLIVHLVAVIAVTKF
HHHHHHEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NANPLEGLLTKVNAPAQFTGFLVALLILSPEGLGALRAVLNNQVQRAMNLFFGSVLATIS
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVPAVTVIATLTGQTLIFGLQTPHIVVMLTVLLLCQLSFSTGRTNVLNGTAHLALFAAY
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MMTIFA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8496184; 8905232; 9278503 [H]