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The map label for this gene is amsH [H]

Identifier: 157371134

GI number: 157371134

Start: 3178710

End: 3179843

Strand: Reverse

Name: amsH [H]

Synonym: Spro_2894

Alternate gene names: 157371134

Gene position: 3179843-3178710 (Counterclockwise)

Preceding gene: 157371135

Following gene: 157371133

Centisome position: 58.36

GC content: 59.88

Gene sequence:

>1134_bases
ATGAACCTTCATTTGCCTCATTGGATGCCACTGGTGCTGTCCAGCGTTTTGCTCAGCGGTTGCACTGCCTTTCCGGGGGC
GCACCTGCCGACCCATGGCAAAGACGTCGTGACTCAGCAGGACGAAGACTTCGATTTGAACAAGCAGGTCCAGGTGTATC
GCGTTACGCCGCTGCTACTGCAGAAAATGCGCCGGGTAGAGCCGGTGGCGCGGCCAAATCTGGCGCTGGACCAGCGTCTG
CAAGGTTATCAGTACCGTATTGGTATCGGCGATGTGCTTAACGTTACCGTCTGGGATCACCCCGAATTGACCACCCCGGC
GGGGCAGTACCGCAGCGCCAGCGACACCGGCAACTGGGTGCATGCCGACGGTTCAATCTTCTACCCCTACATCGGCCGGG
TGAAGGTGGTGGGTAAAACCGTCAGCGAAGTGCGCAACCAGATCGCCGGCCGGTTGGCGACCTATGTGGAAAGTCCACAG
GTCGACGTCAGCATTGCCGCCTTCCGCTCGCAAAAAGTCTATGTGACCGGCGAAGTGAAAACCTCCGGGCAGCAGGCGGT
GACCAACGTGCCGTTGACCATTCTTGATGCGATCAATGCCGCCGGTGGTCTGACAGATAACGCCGACTGGCGCAACGTGG
TGCTGACGCATCAGGGCAAAGAGCAGCCGATTTCCCTGCAGGCGCTGATGCAGCGCGGCGATATCAGCCAGAACCACCTG
CTGGAGCCGGGAGACATCCTGTATGTGCCGCGCAACGACGAACTGAAAGTCTTTGTGATGGGTGAGGTCGGCAAGCAGTC
GACGCTGAAAATGGACCGCAGCGGTATGACGCTGACCGAGGCACTGGGCAGCTCCGAAGGGATCAGCCAAACGGTGGCCG
ACGCCACCGGCGTATTCGTGATCCGCCCGAATGGCGGCAAACAGCCACAGCGGCTGGCGTCGCTGTACCAGTTTGACCTG
TCTGATGCCTCCGCGCTGGCGATGGGCACCGAATTCCAGTTGGAACCTTACGACGTGGTGTACGTCACTGCGGCGCCGAT
CGCGCGCTGGAACCGTTTGATCAGCCAGTTGGCACCGACCATTTCCAGCTTTAACGACCTTAGCGAGGCCACGTTGCGGG
TCCGTAATTGGTAA

Upstream 100 bases:

>100_bases
GCGCGAACCGCGCCAGGCAAGCATTTTTACTGATAATCCTACCGCCGATCGTTAATCCCCAACGGGCCGCAATGGTGGCC
CGTTTTCTCACGGTACCGTT

Downstream 100 bases:

>100_bases
ACCCGTAACGGTGACTTAAGGAATACTGATGATGAAGAACAACGCATTGCCGATTGTGATGGCTGAACAGGACGACAAAC
TGGAGTGGGAGCGCCTGATT

Product: polysaccharide export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNLHLPHWMPLVLSSVLLSGCTAFPGAHLPTHGKDVVTQQDEDFDLNKQVQVYRVTPLLLQKMRRVEPVARPNLALDQRL
QGYQYRIGIGDVLNVTVWDHPELTTPAGQYRSASDTGNWVHADGSIFYPYIGRVKVVGKTVSEVRNQIAGRLATYVESPQ
VDVSIAAFRSQKVYVTGEVKTSGQQAVTNVPLTILDAINAAGGLTDNADWRNVVLTHQGKEQPISLQALMQRGDISQNHL
LEPGDILYVPRNDELKVFVMGEVGKQSTLKMDRSGMTLTEALGSSEGISQTVADATGVFVIRPNGGKQPQRLASLYQFDL
SDASALAMGTEFQLEPYDVVYVTAAPIARWNRLISQLAPTISSFNDLSEATLRVRNW

Sequences:

>Translated_377_residues
MNLHLPHWMPLVLSSVLLSGCTAFPGAHLPTHGKDVVTQQDEDFDLNKQVQVYRVTPLLLQKMRRVEPVARPNLALDQRL
QGYQYRIGIGDVLNVTVWDHPELTTPAGQYRSASDTGNWVHADGSIFYPYIGRVKVVGKTVSEVRNQIAGRLATYVESPQ
VDVSIAAFRSQKVYVTGEVKTSGQQAVTNVPLTILDAINAAGGLTDNADWRNVVLTHQGKEQPISLQALMQRGDISQNHL
LEPGDILYVPRNDELKVFVMGEVGKQSTLKMDRSGMTLTEALGSSEGISQTVADATGVFVIRPNGGKQPQRLASLYQFDL
SDASALAMGTEFQLEPYDVVYVTAAPIARWNRLISQLAPTISSFNDLSEATLRVRNW
>Mature_377_residues
MNLHLPHWMPLVLSSVLLSGCTAFPGAHLPTHGKDVVTQQDEDFDLNKQVQVYRVTPLLLQKMRRVEPVARPNLALDQRL
QGYQYRIGIGDVLNVTVWDHPELTTPAGQYRSASDTGNWVHADGSIFYPYIGRVKVVGKTVSEVRNQIAGRLATYVESPQ
VDVSIAAFRSQKVYVTGEVKTSGQQAVTNVPLTILDAINAAGGLTDNADWRNVVLTHQGKEQPISLQALMQRGDISQNHL
LEPGDILYVPRNDELKVFVMGEVGKQSTLKMDRSGMTLTEALGSSEGISQTVADATGVFVIRPNGGKQPQRLASLYQFDL
SDASALAMGTEFQLEPYDVVYVTAAPIARWNRLISQLAPTISSFNDLSEATLRVRNW

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. Might be involved in the translocation of polysaccharides across the outer membrane [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1788376, Length=372, Percent_Identity=66.9354838709677, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1787218, Length=367, Percent_Identity=64.850136239782, Blast_Score=506, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 41508; Mature: 41508

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHLPHWMPLVLSSVLLSGCTAFPGAHLPTHGKDVVTQQDEDFDLNKQVQVYRVTPLLL
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHH
QKMRRVEPVARPNLALDQRLQGYQYRIGIGDVLNVTVWDHPELTTPAGQYRSASDTGNWV
HHHHHCCCCCCCCCHHHHHCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCEE
HADGSIFYPYIGRVKVVGKTVSEVRNQIAGRLATYVESPQVDVSIAAFRSQKVYVTGEVK
EECCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEC
TSGQQAVTNVPLTILDAINAAGGLTDNADWRNVVLTHQGKEQPISLQALMQRGDISQNHL
CCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCC
LEPGDILYVPRNDELKVFVMGEVGKQSTLKMDRSGMTLTEALGSSEGISQTVADATGVFV
CCCCCEEEECCCCCEEEEEEECCCCCCCEEECCCCCCHHHHHCCCCCCHHHHHCCCCEEE
IRPNGGKQPQRLASLYQFDLSDASALAMGTEFQLEPYDVVYVTAAPIARWNRLISQLAPT
ECCCCCCCHHHHHHHHHHCCCCCCEEEECCEEECCCCEEEEEECCCHHHHHHHHHHHHHH
ISSFNDLSEATLRVRNW
HHHHHHHHHHHEEECCC
>Mature Secondary Structure
MNLHLPHWMPLVLSSVLLSGCTAFPGAHLPTHGKDVVTQQDEDFDLNKQVQVYRVTPLLL
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHH
QKMRRVEPVARPNLALDQRLQGYQYRIGIGDVLNVTVWDHPELTTPAGQYRSASDTGNWV
HHHHHCCCCCCCCCHHHHHCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCEE
HADGSIFYPYIGRVKVVGKTVSEVRNQIAGRLATYVESPQVDVSIAAFRSQKVYVTGEVK
EECCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEC
TSGQQAVTNVPLTILDAINAAGGLTDNADWRNVVLTHQGKEQPISLQALMQRGDISQNHL
CCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCC
LEPGDILYVPRNDELKVFVMGEVGKQSTLKMDRSGMTLTEALGSSEGISQTVADATGVFV
CCCCCEEEECCCCCEEEEEEECCCCCCCEEECCCCCCHHHHHCCCCCCHHHHHCCCCEEE
IRPNGGKQPQRLASLYQFDLSDASALAMGTEFQLEPYDVVYVTAAPIARWNRLISQLAPT
ECCCCCCCHHHHHHHHHHCCCCCCEEEECCEEECCCCEEEEEECCCHHHHHHHHHHHHHH
ISSFNDLSEATLRVRNW
HHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]