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The map label for this gene is appA [H]

Identifier: 157371097

GI number: 157371097

Start: 3135761

End: 3137542

Strand: Reverse

Name: appA [H]

Synonym: Spro_2857

Alternate gene names: 157371097

Gene position: 3137542-3135761 (Counterclockwise)

Preceding gene: 157371098

Following gene: 157371096

Centisome position: 57.58

GC content: 56.45

Gene sequence:

>1782_bases
ATGCCGGTTTCGTTGTTAGACAGCCCTCCGGCCGCCCATTACTCTGCGATAAATTTTGTCACATTTTATCAACGGGTAGA
CAAGGGGCAGTGGGTGAGAAAAATAAAAAATAATATAAACAGACTGTTGCAAAAACGACTGGTGCCCCTGGCGCTGGCTG
GTGCGATAGGCATTTCATTGGGTGCGCAGGCAGAAACGCTGACGGAAGGGATCACTCCGGCCACCGACGCCAGCCTGGTA
CCGAATGCTGCCAAACTGCGTAAAGACACCGTGGTGGCCGGTATTCTCGAGCCACAGGGCAACTTTAACCCTTATCTGTT
CACCAACGGTTGGGATGAGAACGTCACCGACGTGATCTTCACCCGGCTGATTGGCCTGGACAGCCAGGGTAAACCGGTCG
CACGGCTGGCGGAGTCCTGGCAGGTCAGCCCGGATAACCGGGTGTATACCATCAAGCTGCGCCCGAACCTGCTCTACAGC
GACGGCTCGCCGATCAAGGCCGAAGACATCGCCTTTACCCTGACGCTGCTGCATGACCCGGCCTATGACGGTGAAACCGA
TATTACCCCGGCGCATATTCAGGGCGGTGCGGAATACAAAAACGGCACCGCCCCCAGCATCAGCGGGTTGAAGGTGATTG
ACGATCGCACTCTGCAGATCACCACCACCCAGCCGGGTGCGACCACGCTGCAACTGATCGGCGGGCCGGTGTTGTCGAAA
GCCTACTATGGCAAGGATTATCAACGCGGCAAGCTGGAAAGCGTGCGCGCGTTGAATGGTAAGCCGTTGGGCAACGGCCC
CTATATTTACGACAAATACATTCCGGGGCAGGAAATCCGCTTCCACGCCAACCCGAATTTCTACCTCGGCCAGCCGGCGG
TGGCGCGCTTTATTTACCGCGTGACCAACCCGGCCACCAACTTCCAGCTGTTCCAGACCGGTGAAACCGACTATGACGCC
TTCACCTCCAAGCCCGATGACATTGAACAGTTGAAACTGTTGGGGTTCGCCAACATCAATTTGTACAGCTCAAGCGACTA
CAGCCGGGTGGATTTCAACTTTAAGCGCCCGGCGTTGCAGGACGTGCGTGTCCGTCAGGCATTGATCTACGGTTTGGATC
GCCAAAAACTGATTACCGTGGTGTATCAGGGATATGGCTCAGTGGCTAACCAGCCGATCTCACCCGTTTCCTGGGCCTAT
GATCCTAAGGGGATCAATCCATACGTCTATGACGTCAACAAAGCGAAACAATTGCTCGATCAGGCCGGGTGGAAACCGGG
TGCAGACGGCATTCGCCAGAAAGACGGCAAGAAACTGGAACTGACGCTGCTGGTGACCAAAAAGCTGCTTAACGATGCGC
TGGTGCCGATCGCCAAAGACAACTATCGGCAGATAGGCGTGCTGCTCAAACCGCAGGTGTTGGATTTCAACGCATTGCTG
GCACAGCGCAAGGCCGGGAATTATGACCTGGCATCGCTCAGTACCAGCGGGTTGAATGACCCGCATGACGGGGTGCGCGA
TTTCATCAGCCAGGTGAGTGAAACCGGCTATCACAACCCGCAGGCAGACCAACTGATCGGTCAGGCTAACGCCACGCTGG
ATATCGGGCAGCGCAAACCGCTGTATCACCAACTGTATCAGGTGCTGGCGGAAGACCCACCGGTGATCCTGCTGGGTAAC
CGCAAAGTGCTCAGCGCCAGCAGCGCCCGCGTCACCGGCTTTAAACCGGATGTCTACAATGGCCTGACCGGCAGCCTGCC
GGACGTCAAACTGGCTCAGTAA

Upstream 100 bases:

>100_bases
CTTAGCCAAACTTATCCGGCAGCTCGAACCGGCAGGCTGAACAGCGCCAACGGCGGGGCATCGCAATAGAAAAATTCATA
ATGCTTTTTTTGGCAATGGA

Downstream 100 bases:

>100_bases
CGGCAAGGTACCGCCGCGCTCCTCCGTGCGGCGGTAATGGAAAACACCATGAGAAATTTTATCCTGCGCCGCCTGCTGCA
AACCCTGCCGATGCTGCTGC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MPVSLLDSPPAAHYSAINFVTFYQRVDKGQWVRKIKNNINRLLQKRLVPLALAGAIGISLGAQAETLTEGITPATDASLV
PNAAKLRKDTVVAGILEPQGNFNPYLFTNGWDENVTDVIFTRLIGLDSQGKPVARLAESWQVSPDNRVYTIKLRPNLLYS
DGSPIKAEDIAFTLTLLHDPAYDGETDITPAHIQGGAEYKNGTAPSISGLKVIDDRTLQITTTQPGATTLQLIGGPVLSK
AYYGKDYQRGKLESVRALNGKPLGNGPYIYDKYIPGQEIRFHANPNFYLGQPAVARFIYRVTNPATNFQLFQTGETDYDA
FTSKPDDIEQLKLLGFANINLYSSSDYSRVDFNFKRPALQDVRVRQALIYGLDRQKLITVVYQGYGSVANQPISPVSWAY
DPKGINPYVYDVNKAKQLLDQAGWKPGADGIRQKDGKKLELTLLVTKKLLNDALVPIAKDNYRQIGVLLKPQVLDFNALL
AQRKAGNYDLASLSTSGLNDPHDGVRDFISQVSETGYHNPQADQLIGQANATLDIGQRKPLYHQLYQVLAEDPPVILLGN
RKVLSASSARVTGFKPDVYNGLTGSLPDVKLAQ

Sequences:

>Translated_593_residues
MPVSLLDSPPAAHYSAINFVTFYQRVDKGQWVRKIKNNINRLLQKRLVPLALAGAIGISLGAQAETLTEGITPATDASLV
PNAAKLRKDTVVAGILEPQGNFNPYLFTNGWDENVTDVIFTRLIGLDSQGKPVARLAESWQVSPDNRVYTIKLRPNLLYS
DGSPIKAEDIAFTLTLLHDPAYDGETDITPAHIQGGAEYKNGTAPSISGLKVIDDRTLQITTTQPGATTLQLIGGPVLSK
AYYGKDYQRGKLESVRALNGKPLGNGPYIYDKYIPGQEIRFHANPNFYLGQPAVARFIYRVTNPATNFQLFQTGETDYDA
FTSKPDDIEQLKLLGFANINLYSSSDYSRVDFNFKRPALQDVRVRQALIYGLDRQKLITVVYQGYGSVANQPISPVSWAY
DPKGINPYVYDVNKAKQLLDQAGWKPGADGIRQKDGKKLELTLLVTKKLLNDALVPIAKDNYRQIGVLLKPQVLDFNALL
AQRKAGNYDLASLSTSGLNDPHDGVRDFISQVSETGYHNPQADQLIGQANATLDIGQRKPLYHQLYQVLAEDPPVILLGN
RKVLSASSARVTGFKPDVYNGLTGSLPDVKLAQ
>Mature_592_residues
PVSLLDSPPAAHYSAINFVTFYQRVDKGQWVRKIKNNINRLLQKRLVPLALAGAIGISLGAQAETLTEGITPATDASLVP
NAAKLRKDTVVAGILEPQGNFNPYLFTNGWDENVTDVIFTRLIGLDSQGKPVARLAESWQVSPDNRVYTIKLRPNLLYSD
GSPIKAEDIAFTLTLLHDPAYDGETDITPAHIQGGAEYKNGTAPSISGLKVIDDRTLQITTTQPGATTLQLIGGPVLSKA
YYGKDYQRGKLESVRALNGKPLGNGPYIYDKYIPGQEIRFHANPNFYLGQPAVARFIYRVTNPATNFQLFQTGETDYDAF
TSKPDDIEQLKLLGFANINLYSSSDYSRVDFNFKRPALQDVRVRQALIYGLDRQKLITVVYQGYGSVANQPISPVSWAYD
PKGINPYVYDVNKAKQLLDQAGWKPGADGIRQKDGKKLELTLLVTKKLLNDALVPIAKDNYRQIGVLLKPQVLDFNALLA
QRKAGNYDLASLSTSGLNDPHDGVRDFISQVSETGYHNPQADQLIGQANATLDIGQRKPLYHQLYQVLAEDPPVILLGNR
KVLSASSARVTGFKPDVYNGLTGSLPDVKLAQ

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=524, Percent_Identity=26.5267175572519, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1789397, Length=477, Percent_Identity=25.7861635220126, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787052, Length=528, Percent_Identity=26.5151515151515, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789887, Length=472, Percent_Identity=25.4237288135593, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787495, Length=542, Percent_Identity=23.9852398523985, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787762, Length=512, Percent_Identity=24.0234375, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787551, Length=533, Percent_Identity=21.7636022514071, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081878, Length=462, Percent_Identity=25.3246753246753, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 65247; Mature: 65116

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSLLDSPPAAHYSAINFVTFYQRVDKGQWVRKIKNNINRLLQKRLVPLALAGAIGISL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAQAETLTEGITPATDASLVPNAAKLRKDTVVAGILEPQGNFNPYLFTNGWDENVTDVIF
CCCHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCHHHHHH
TRLIGLDSQGKPVARLAESWQVSPDNRVYTIKLRPNLLYSDGSPIKAEDIAFTLTLLHDP
HHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCCCEECCEEEEEEEEECC
AYDGETDITPAHIQGGAEYKNGTAPSISGLKVIDDRTLQITTTQPGATTLQLIGGPVLSK
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEECCCHHHHH
AYYGKDYQRGKLESVRALNGKPLGNGPYIYDKYIPGQEIRFHANPNFYLGQPAVARFIYR
HHCCCCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCEEECCHHHHHHHHH
VTNPATNFQLFQTGETDYDAFTSKPDDIEQLKLLGFANINLYSSSDYSRVDFNFKRPALQ
HCCCCCCEEEEECCCCCCHHHCCCCCCHHHHHHEEEEEEEEECCCCCCEEECCCCCCCHH
DVRVRQALIYGLDRQKLITVVYQGYGSVANQPISPVSWAYDPKGINPYVYDVNKAKQLLD
HHHHHHHHHHCCCHHHEEEHHHHCCCHHCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHH
QAGWKPGADGIRQKDGKKLELTLLVTKKLLNDALVPIAKDNYRQIGVLLKPQVLDFNALL
HCCCCCCCCCHHHCCCCEEEEEEEEHHHHHHHCCCCCCCCCCEEEEEEECCCEECHHHHH
AQRKAGNYDLASLSTSGLNDPHDGVRDFISQVSETGYHNPQADQLIGQANATLDIGQRKP
HHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEECCCCCC
LYHQLYQVLAEDPPVILLGNRKVLSASSARVTGFKPDVYNGLTGSLPDVKLAQ
HHHHHHHHHHCCCCEEEECCCEEEECCCCEEECCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVSLLDSPPAAHYSAINFVTFYQRVDKGQWVRKIKNNINRLLQKRLVPLALAGAIGISL
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAQAETLTEGITPATDASLVPNAAKLRKDTVVAGILEPQGNFNPYLFTNGWDENVTDVIF
CCCHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCHHHHHH
TRLIGLDSQGKPVARLAESWQVSPDNRVYTIKLRPNLLYSDGSPIKAEDIAFTLTLLHDP
HHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCCCEECCEEEEEEEEECC
AYDGETDITPAHIQGGAEYKNGTAPSISGLKVIDDRTLQITTTQPGATTLQLIGGPVLSK
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEECCCHHHHH
AYYGKDYQRGKLESVRALNGKPLGNGPYIYDKYIPGQEIRFHANPNFYLGQPAVARFIYR
HHCCCCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCEEECCHHHHHHHHH
VTNPATNFQLFQTGETDYDAFTSKPDDIEQLKLLGFANINLYSSSDYSRVDFNFKRPALQ
HCCCCCCEEEEECCCCCCHHHCCCCCCHHHHHHEEEEEEEEECCCCCCEEECCCCCCCHH
DVRVRQALIYGLDRQKLITVVYQGYGSVANQPISPVSWAYDPKGINPYVYDVNKAKQLLD
HHHHHHHHHHCCCHHHEEEHHHHCCCHHCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHH
QAGWKPGADGIRQKDGKKLELTLLVTKKLLNDALVPIAKDNYRQIGVLLKPQVLDFNALL
HCCCCCCCCCHHHCCCCEEEEEEEEHHHHHHHCCCCCCCCCCEEEEEEECCCEECHHHHH
AQRKAGNYDLASLSTSGLNDPHDGVRDFISQVSETGYHNPQADQLIGQANATLDIGQRKP
HHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEECCCCCC
LYHQLYQVLAEDPPVILLGNRKVLSASSARVTGFKPDVYNGLTGSLPDVKLAQ
HHHHHHHHHHCCCCEEEECCCEEEECCCCEEECCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]