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The map label for this gene is lacF [H]

Identifier: 157371086

GI number: 157371086

Start: 3124343

End: 3125284

Strand: Direct

Name: lacF [H]

Synonym: Spro_2846

Alternate gene names: 157371086

Gene position: 3124343-3125284 (Clockwise)

Preceding gene: 157371085

Following gene: 157371087

Centisome position: 57.34

GC content: 55.31

Gene sequence:

>942_bases
ATGCGTAAGCGATTTTTCAAAGGGCTGCTGCACGATCACCGCACCGCCTACCTGCTGCTGCTGCCGTTTATGCTGCTGTT
CGCGGTGTTCAACGCCTTCCCGATCTTCTTCTCGGCGTTTTTGTCATTTCAGTCCTGGAACCCGGTAAAGGGCCTGAGTT
CGATGAAGTTTGTCGGGCTGGATAACTTTTACTATGCCTTCACCGACGATGCCTTCTGGGTGTCGCTGGTCAACACCATC
AAAATTACGCTGGTGTCCGGCCTGTGCCAGCACCTGTTCGCCATCACCCTGGCCTACTCATTGCTGCACATCATCGTGCG
CGGCAGACGCTTTTTGAAGGCGGCGATCTTCCTGCCCTACATCACCTCGTCCGTCGCCGTCAGCCTGATTGTGTTTAACC
TGTTCTCCCCGGTCGGCATGGTTAACGAACTGCTCAGCGGGCTAGCCACCAAACTGCAACTCGCAACCTTGGCAGCGGCG
TTGCCCATCAACTTCTTTGACAGTGACTGGATCCGCTTCACCATCGCCAATCAGGTGACCTGGAAGTTTACCGGCATCAA
TACGGTGATCTACATGACCGGTCTGGCGTCGATCTCACCGGACCTGTACGAAGCGATCGAACTGGACGGTGCCGGCCGCT
GGCAAAAATTTCGTTATATCGCCCTGCCGCTGTTGGCGCCCTTTATCTTCTTCGCCACCTTGATGACGCTGATCGGCAAT
ATGCAATTGTTCAATGAACCGATGATCCTCACGCAAGGCACCGGCGGCGTCGACAACAGCGGCCTGACGGTATCGATGTA
CGTGTACAAAACCGGTTGGTCCTATCTGGACATGGGTACCGCTTCGGCGATCTCCTGGATCCTGTTCCTGTTGATTGCCG
TGGTCAGCGCCCTGGTGTTCTGGGGCTTTGGCAAGCGTGGAGGATTGAACAATGAACGCTAA

Upstream 100 bases:

>100_bases
GGAAACACGAATAACCTTCCCCCCTCTCCTCCAGAAAGAGGCGATATTCAGTCAGGCCTTTCGGCCACCCATGTTCGTTT
TCCGTCGGTTCGGGGAGTCT

Downstream 100 bases:

>100_bases
CGGCCGCCGTTTTGACACCGGCAAACTGGTGATTTACCTGCTGGTCTCACTGTTTGCTTCGCTGTCATTATTCCCGTTCT
ATTGGTCGGCCTTGCTGGCG

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRKRFFKGLLHDHRTAYLLLLPFMLLFAVFNAFPIFFSAFLSFQSWNPVKGLSSMKFVGLDNFYYAFTDDAFWVSLVNTI
KITLVSGLCQHLFAITLAYSLLHIIVRGRRFLKAAIFLPYITSSVAVSLIVFNLFSPVGMVNELLSGLATKLQLATLAAA
LPINFFDSDWIRFTIANQVTWKFTGINTVIYMTGLASISPDLYEAIELDGAGRWQKFRYIALPLLAPFIFFATLMTLIGN
MQLFNEPMILTQGTGGVDNSGLTVSMYVYKTGWSYLDMGTASAISWILFLLIAVVSALVFWGFGKRGGLNNER

Sequences:

>Translated_313_residues
MRKRFFKGLLHDHRTAYLLLLPFMLLFAVFNAFPIFFSAFLSFQSWNPVKGLSSMKFVGLDNFYYAFTDDAFWVSLVNTI
KITLVSGLCQHLFAITLAYSLLHIIVRGRRFLKAAIFLPYITSSVAVSLIVFNLFSPVGMVNELLSGLATKLQLATLAAA
LPINFFDSDWIRFTIANQVTWKFTGINTVIYMTGLASISPDLYEAIELDGAGRWQKFRYIALPLLAPFIFFATLMTLIGN
MQLFNEPMILTQGTGGVDNSGLTVSMYVYKTGWSYLDMGTASAISWILFLLIAVVSALVFWGFGKRGGLNNER
>Mature_313_residues
MRKRFFKGLLHDHRTAYLLLLPFMLLFAVFNAFPIFFSAFLSFQSWNPVKGLSSMKFVGLDNFYYAFTDDAFWVSLVNTI
KITLVSGLCQHLFAITLAYSLLHIIVRGRRFLKAAIFLPYITSSVAVSLIVFNLFSPVGMVNELLSGLATKLQLATLAAA
LPINFFDSDWIRFTIANQVTWKFTGINTVIYMTGLASISPDLYEAIELDGAGRWQKFRYIALPLLAPFIFFATLMTLIGN
MQLFNEPMILTQGTGGVDNSGLTVSMYVYKTGWSYLDMGTASAISWILFLLIAVVSALVFWGFGKRGGLNNER

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=290, Percent_Identity=31.0344827586207, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787570, Length=263, Percent_Identity=30.0380228136882, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35122; Mature: 35122

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRFFKGLLHDHRTAYLLLLPFMLLFAVFNAFPIFFSAFLSFQSWNPVKGLSSMKFVGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEC
DNFYYAFTDDAFWVSLVNTIKITLVSGLCQHLFAITLAYSLLHIIVRGRRFLKAAIFLPY
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITSSVAVSLIVFNLFSPVGMVNELLSGLATKLQLATLAAALPINFFDSDWIRFTIANQVT
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEE
WKFTGINTVIYMTGLASISPDLYEAIELDGAGRWQKFRYIALPLLAPFIFFATLMTLIGN
EEEECCCEEEEEHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
MQLFNEPMILTQGTGGVDNSGLTVSMYVYKTGWSYLDMGTASAISWILFLLIAVVSALVF
HHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHH
WGFGKRGGLNNER
HCCCCCCCCCCCC
>Mature Secondary Structure
MRKRFFKGLLHDHRTAYLLLLPFMLLFAVFNAFPIFFSAFLSFQSWNPVKGLSSMKFVGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEC
DNFYYAFTDDAFWVSLVNTIKITLVSGLCQHLFAITLAYSLLHIIVRGRRFLKAAIFLPY
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITSSVAVSLIVFNLFSPVGMVNELLSGLATKLQLATLAAALPINFFDSDWIRFTIANQVT
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEE
WKFTGINTVIYMTGLASISPDLYEAIELDGAGRWQKFRYIALPLLAPFIFFATLMTLIGN
EEEECCCEEEEEHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
MQLFNEPMILTQGTGGVDNSGLTVSMYVYKTGWSYLDMGTASAISWILFLLIAVVSALVF
HHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHH
WGFGKRGGLNNER
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]