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The map label for this gene is ruvC

Identifier: 157371018

GI number: 157371018

Start: 3058643

End: 3059164

Strand: Reverse

Name: ruvC

Synonym: Spro_2778

Alternate gene names: 157371018

Gene position: 3059164-3058643 (Counterclockwise)

Preceding gene: 157371019

Following gene: 157371017

Centisome position: 56.14

GC content: 58.05

Gene sequence:

>522_bases
ATGACGATCATTCTCGGTATCGATCCCGGATCGCGCGTCACCGGTTACGGCCTTATTCGCCAGCAGGGACGCCAGTTGAC
CTATGTGGCCAGCGGCTGTATCCGCACCGTGGTGGATGATATGCCGACGCGTTTGAAGCTGATTTATGCGGGCGTCAGCG
AAATCATCACCCAGTTTAAACCGGATTTTTTTGCCATCGAACAGGTGTTTATGGCGAAAAACCCGGATTCGGCGCTCAAA
CTCGGGCAGGCGCGCGGTGTGGCCATTGTCGCCGCCGTGAACCAGGATTTGGAAGTGTTTGAGTATGCGGCACGCCAGGT
GAAACAAACGGTGGTCGGTACCGGTGCGGCAGAAAAAGCGCAGGTGCAGCATATGGTGCGCTCCTTGCTGAAACTGTCGG
CCAACCCTCAGGCTGATGCCGCCGATGCGCTGGCCATCGCCATCACCCACTGCCACCTCAGCCAGAACGTGCTGCGGATG
AGTGAAGGGCGCTTGAACCTGGCGCGTGGGCGTTTAAAGTAG

Upstream 100 bases:

>100_bases
TCCGACGAAGTCGCGGCAACGCTGTAATCGGCTTGAAGTACGGCGCTGCCTGGTGGCAGCGCCTGGCGAAGTTAATGCTT
AACGCAAGGTGGGTGTAGGG

Downstream 100 bases:

>100_bases
CGCTTGATCGGTGATATAACAGGCTCCAGATAAAGTTTGTTATTAGGGAGAGCGTGCCGAAGGGGTCGTAGCGGCTTGTC
CGCCGGAGCGCCCCTCGGTG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MTIILGIDPGSRVTGYGLIRQQGRQLTYVASGCIRTVVDDMPTRLKLIYAGVSEIITQFKPDFFAIEQVFMAKNPDSALK
LGQARGVAIVAAVNQDLEVFEYAARQVKQTVVGTGAAEKAQVQHMVRSLLKLSANPQADAADALAIAITHCHLSQNVLRM
SEGRLNLARGRLK

Sequences:

>Translated_173_residues
MTIILGIDPGSRVTGYGLIRQQGRQLTYVASGCIRTVVDDMPTRLKLIYAGVSEIITQFKPDFFAIEQVFMAKNPDSALK
LGQARGVAIVAAVNQDLEVFEYAARQVKQTVVGTGAAEKAQVQHMVRSLLKLSANPQADAADALAIAITHCHLSQNVLRM
SEGRLNLARGRLK
>Mature_172_residues
TIILGIDPGSRVTGYGLIRQQGRQLTYVASGCIRTVVDDMPTRLKLIYAGVSEIITQFKPDFFAIEQVFMAKNPDSALKL
GQARGVAIVAAVNQDLEVFEYAARQVKQTVVGTGAAEKAQVQHMVRSLLKLSANPQADAADALAIAITHCHLSQNVLRMS
EGRLNLARGRLK

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=173, Percent_Identity=84.9710982658959, Blast_Score=285, Evalue=8e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_SERP5 (A8GFI9)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479007.1
- ProteinModelPortal:   A8GFI9
- SMR:   A8GFI9
- STRING:   A8GFI9
- GeneID:   5603993
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2778
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- BioCyc:   SPRO399741:SPRO_2778-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18777; Mature: 18646

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIILGIDPGSRVTGYGLIRQQGRQLTYVASGCIRTVVDDMPTRLKLIYAGVSEIITQFK
CEEEEECCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
PDFFAIEQVFMAKNPDSALKLGQARGVAIVAAVNQDLEVFEYAARQVKQTVVGTGAAEKA
CCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHH
QVQHMVRSLLKLSANPQADAADALAIAITHCHLSQNVLRMSEGRLNLARGRLK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC
>Mature Secondary Structure 
TIILGIDPGSRVTGYGLIRQQGRQLTYVASGCIRTVVDDMPTRLKLIYAGVSEIITQFK
EEEEECCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
PDFFAIEQVFMAKNPDSALKLGQARGVAIVAAVNQDLEVFEYAARQVKQTVVGTGAAEKA
CCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHH
QVQHMVRSLLKLSANPQADAADALAIAITHCHLSQNVLRMSEGRLNLARGRLK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA