Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 157370994

GI number: 157370994

Start: 3034508

End: 3035548

Strand: Reverse

Name: mltB [H]

Synonym: Spro_2754

Alternate gene names: 157370994

Gene position: 3035548-3034508 (Counterclockwise)

Preceding gene: 157370995

Following gene: 157370993

Centisome position: 55.71

GC content: 56.48

Gene sequence:

>1041_bases
ATGAGAGGGTCAGCGAAGACAGCCATAGTGGCTCTGCTGGCGCTGGGAACTGGCGTCTGTCCGCAACTGATGGCGCAGGT
CGCTTCACCGGCCACAACTGCTGCGCCAGCACAAGGCACGTTGGCTGAAACCGGTCGCGATCCGGCACAGTTCCCAGCCT
ATGTAGAACAACTGAAACGTCAGGCGCTGGCGCAGGGCATTAGCCCGGCTATCGTCGCCAGCGCTTTTGCCAACGTGCAT
TTTGTCGACCGAGTGATTAAGGCAGACCGTAATCAGCCGGAAAAGAAAATCACGCTGGATGATTATCTAAAACGTGTATT
GCCGCCGGCCAAGATAGCGCAGGGCCGCGCTCTTTACCGGCAATATCAACCCCAGTTGTCTCGTGTTACCGCTCACTACG
GTGTTCCTGGGCGTTATGTGGTGGCGCTGTGGGGGATGGAAAGCGCCTTCGGCAAGATCCAGGGGCGTGAAGATGTGATA
TCTGCGCTGGCTACGCTGGCCTTTGAAGGTCGCCGTGAAGCTTTCTTCAGCCAACAGCTAATGGCAGCACTGCGGATCCT
CGAACAGGGGCATGTCAGCCGTGAACAACTGAAAGGCTCCTGGGCCGGTGCGATGGGGCAGTGCCAGTTTATGCCGACCT
CATTCCTGAATTATGCAGCGGATGGCGATGGCCGTATTGATATTTGGAACAACGTTGATGATGTGTTTGCCTCTACGGCC
CGTTATCTGTCGCGTGAAGGTTGGCAACCGGGCGTAGGCTGGGGCAGGGAAGTTAAACTGCCTGTCGGTTTCAGCAGTGA
CAGCCTGGGGCTGAAAGTGAGCCAGGCCCACAGCGTCAATGAATGGCAAAAACGTGGCGTGCGTCGTGCGGATGGTTCAG
CCTTGCCGCATTCTACCCTGCGCAGTTGGGTGATCACGCCGGATGACATGCAGGGCCGGGCGTTCATGGTTTATGACAAT
TTTCGCACGATCATGCACTGGAATCGCTCTTACTATTTTGCCATCGGTGTGGGTATGATGGCTGACGGTATCGGGCAATA
A

Upstream 100 bases:

>100_bases
TTTATTAAAACAGCACTTGTCTGACGACAAAAAATAACCGTCGTTGGCGTACAGGGGCTACGCTGGATCAGTAACCCCAA
ACCAACAAAGGAGCTGGGTG

Downstream 100 bases:

>100_bases
ATTAGGGCCAGAGTACCTCCACCCGCGCGTGGGATAACGGGCTTAGCAAGCTAAGCCCCTACATGATTGTAGGGAGATGC
GGTATGTATCAACATAGAGA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRGSAKTAIVALLALGTGVCPQLMAQVASPATTAAPAQGTLAETGRDPAQFPAYVEQLKRQALAQGISPAIVASAFANVH
FVDRVIKADRNQPEKKITLDDYLKRVLPPAKIAQGRALYRQYQPQLSRVTAHYGVPGRYVVALWGMESAFGKIQGREDVI
SALATLAFEGRREAFFSQQLMAALRILEQGHVSREQLKGSWAGAMGQCQFMPTSFLNYAADGDGRIDIWNNVDDVFASTA
RYLSREGWQPGVGWGREVKLPVGFSSDSLGLKVSQAHSVNEWQKRGVRRADGSALPHSTLRSWVITPDDMQGRAFMVYDN
FRTIMHWNRSYYFAIGVGMMADGIGQ

Sequences:

>Translated_346_residues
MRGSAKTAIVALLALGTGVCPQLMAQVASPATTAAPAQGTLAETGRDPAQFPAYVEQLKRQALAQGISPAIVASAFANVH
FVDRVIKADRNQPEKKITLDDYLKRVLPPAKIAQGRALYRQYQPQLSRVTAHYGVPGRYVVALWGMESAFGKIQGREDVI
SALATLAFEGRREAFFSQQLMAALRILEQGHVSREQLKGSWAGAMGQCQFMPTSFLNYAADGDGRIDIWNNVDDVFASTA
RYLSREGWQPGVGWGREVKLPVGFSSDSLGLKVSQAHSVNEWQKRGVRRADGSALPHSTLRSWVITPDDMQGRAFMVYDN
FRTIMHWNRSYYFAIGVGMMADGIGQ
>Mature_346_residues
MRGSAKTAIVALLALGTGVCPQLMAQVASPATTAAPAQGTLAETGRDPAQFPAYVEQLKRQALAQGISPAIVASAFANVH
FVDRVIKADRNQPEKKITLDDYLKRVLPPAKIAQGRALYRQYQPQLSRVTAHYGVPGRYVVALWGMESAFGKIQGREDVI
SALATLAFEGRREAFFSQQLMAALRILEQGHVSREQLKGSWAGAMGQCQFMPTSFLNYAADGDGRIDIWNNVDDVFASTA
RYLSREGWQPGVGWGREVKLPVGFSSDSLGLKVSQAHSVNEWQKRGVRRADGSALPHSTLRSWVITPDDMQGRAFMVYDN
FRTIMHWNRSYYFAIGVGMMADGIGQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=258, Percent_Identity=31.0077519379845, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 37984; Mature: 37984

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGSAKTAIVALLALGTGVCPQLMAQVASPATTAAPAQGTLAETGRDPAQFPAYVEQLKR
CCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
QALAQGISPAIVASAFANVHFVDRVIKADRNQPEKKITLDDYLKRVLPPAKIAQGRALYR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCHHHHHHHHHHHH
QYQPQLSRVTAHYGVPGRYVVALWGMESAFGKIQGREDVISALATLAFEGRREAFFSQQL
HHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHH
MAALRILEQGHVSREQLKGSWAGAMGQCQFMPTSFLNYAADGDGRIDIWNNVDDVFASTA
HHHHHHHHHCCCCHHHHCCHHCCCCCCEEECCHHHHHHCCCCCCCEEECCCHHHHHHHHH
RYLSREGWQPGVGWGREVKLPVGFSSDSLGLKVSQAHSVNEWQKRGVRRADGSALPHSTL
HHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEHHHCCHHHHHHCCCCCCCCCCCCHHHH
RSWVITPDDMQGRAFMVYDNFRTIMHWNRSYYFAIGVGMMADGIGQ
HHCCCCCCCCCCCEEEEECCCHHHHHCCCCEEEEEEHHHHHCCCCC
>Mature Secondary Structure
MRGSAKTAIVALLALGTGVCPQLMAQVASPATTAAPAQGTLAETGRDPAQFPAYVEQLKR
CCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
QALAQGISPAIVASAFANVHFVDRVIKADRNQPEKKITLDDYLKRVLPPAKIAQGRALYR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCHHHHHHHHHHHH
QYQPQLSRVTAHYGVPGRYVVALWGMESAFGKIQGREDVISALATLAFEGRREAFFSQQL
HHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHH
MAALRILEQGHVSREQLKGSWAGAMGQCQFMPTSFLNYAADGDGRIDIWNNVDDVFASTA
HHHHHHHHHCCCCHHHHCCHHCCCCCCEEECCHHHHHHCCCCCCCEEECCCHHHHHHHHH
RYLSREGWQPGVGWGREVKLPVGFSSDSLGLKVSQAHSVNEWQKRGVRRADGSALPHSTL
HHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEHHHCCHHHHHHCCCCCCCCCCCCHHHH
RSWVITPDDMQGRAFMVYDNFRTIMHWNRSYYFAIGVGMMADGIGQ
HHCCCCCCCCCCCEEEEECCCHHHHHCCCCEEEEEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]