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The map label for this gene is mipA [H]

Identifier: 157370970

GI number: 157370970

Start: 3006717

End: 3007481

Strand: Reverse

Name: mipA [H]

Synonym: Spro_2730

Alternate gene names: 157370970

Gene position: 3007481-3006717 (Counterclockwise)

Preceding gene: 157370973

Following gene: 157370967

Centisome position: 55.19

GC content: 50.33

Gene sequence:

>765_bases
ATGGAAAAAGAACCAGTGAAGATTTTTAAACTCAAAACGCTAGCAATTATCGCTTCCGCAATGGTTTGTGCGCAAAGTGC
TCAGGCAGGAACTTGGTCACTCGGCGCTTCCGCGCTGGTTAGCCCGGATCCGTATCGTGGCTATCAGGACCGCGTGTATC
CGGTACCCGTCATTAACTATGAAGGTGACGATTTCTACTTCCGTACGCTGACCGCCGGTTATTACCTGTGGAAAGATCAG
CAAAACCAACTGAGTCTGATGGGCTACTTTTCACCGCTGGGCTATCGCCCGGGTGACAGCGACGATGACCGCATGAAGCA
GTTGAACAAGCGTCGCGGTACGCTGATGGCCGGTCTGGCCTATTCGCATTTTGCTGAGTGGGGGATTCTGCGCACCACCT
TTACCGGTGATACGTTAGACTACAGCAACGGTATGGTGGGTGATTTAGCCTATCTGTACAAATTTGATCTGGGCGATTTC
ACCCTGGTACCTGGTGTGGGTGTGTCGTGGAGCAGCAAAAACCAGAACAAATACTACTACGGTATCAGCGAAAATGAATC
TCGCCGCAGCGGTCTGGACAGTTACACGCCAAGTGATAGCTGGGCGCCGTACGTTGAGCTGAGTGCCAACTATAAGATCA
ACAAAGATTGGGATGCCTTCTTTGTTGGGCGTTATGTCCAACTGTCCGATGAAGTTAAAGACAGCCCAATGGTGGATAAA
TCTTACACCGGACTGTTGATGACGGGTGTGAAATACAGCTTCTAA

Upstream 100 bases:

>100_bases
TAGGTTCTAAGTTATTCGATCTCGGTCGTTTTCTATTGTAAAGATTCAGCGCCTAACTAAATTCTCACATAAACTAAGCA
CGCTATTAACTCTGTTATTT

Downstream 100 bases:

>100_bases
TCGAAGCCTGCACCAATGTTGTGCAATGTAGGCATCATAAAGGGGCGCTGGCGCCCCTTTTGCACATCTGTAGCGCAGCA
AGTCTTTACTGCACTTAAAA

Product: MltA-interacting MipA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MEKEPVKIFKLKTLAIIASAMVCAQSAQAGTWSLGASALVSPDPYRGYQDRVYPVPVINYEGDDFYFRTLTAGYYLWKDQ
QNQLSLMGYFSPLGYRPGDSDDDRMKQLNKRRGTLMAGLAYSHFAEWGILRTTFTGDTLDYSNGMVGDLAYLYKFDLGDF
TLVPGVGVSWSSKNQNKYYYGISENESRRSGLDSYTPSDSWAPYVELSANYKINKDWDAFFVGRYVQLSDEVKDSPMVDK
SYTGLLMTGVKYSF

Sequences:

>Translated_254_residues
MEKEPVKIFKLKTLAIIASAMVCAQSAQAGTWSLGASALVSPDPYRGYQDRVYPVPVINYEGDDFYFRTLTAGYYLWKDQ
QNQLSLMGYFSPLGYRPGDSDDDRMKQLNKRRGTLMAGLAYSHFAEWGILRTTFTGDTLDYSNGMVGDLAYLYKFDLGDF
TLVPGVGVSWSSKNQNKYYYGISENESRRSGLDSYTPSDSWAPYVELSANYKINKDWDAFFVGRYVQLSDEVKDSPMVDK
SYTGLLMTGVKYSF
>Mature_254_residues
MEKEPVKIFKLKTLAIIASAMVCAQSAQAGTWSLGASALVSPDPYRGYQDRVYPVPVINYEGDDFYFRTLTAGYYLWKDQ
QNQLSLMGYFSPLGYRPGDSDDDRMKQLNKRRGTLMAGLAYSHFAEWGILRTTFTGDTLDYSNGMVGDLAYLYKFDLGDF
TLVPGVGVSWSSKNQNKYYYGISENESRRSGLDSYTPSDSWAPYVELSANYKINKDWDAFFVGRYVQLSDEVKDSPMVDK
SYTGLLMTGVKYSF

Specific function: May serve as a scaffold protein required for the formation of a complex with mrcB/ponB and mltA, this complex could play a role in enlargement and septation of the murein sacculus [H]

COG id: COG3713

COG function: function code M; Outer membrane protein V

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mipA/ompV family [H]

Homologues:

Organism=Escherichia coli, GI1788082, Length=246, Percent_Identity=54.8780487804878, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1790010, Length=235, Percent_Identity=42.9787234042553, Blast_Score=204, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010583 [H]

Pfam domain/function: PF06629 MipA [H]

EC number: NA

Molecular weight: Translated: 28751; Mature: 28751

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEPVKIFKLKTLAIIASAMVCAQSAQAGTWSLGASALVSPDPYRGYQDRVYPVPVINY
CCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCCCCCCCCCCEEEEEEEEC
EGDDFYFRTLTAGYYLWKDQQNQLSLMGYFSPLGYRPGDSDDDRMKQLNKRRGTLMAGLA
CCCCEEEEEEECEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEHHH
YSHFAEWGILRTTFTGDTLDYSNGMVGDLAYLYKFDLGDFTLVPGVGVSWSSKNQNKYYY
HHHHHHCCEEEEEECCCCEECCCCCHHHEEEEEEECCCCEEECCCCCCEECCCCCCEEEE
GISENESRRSGLDSYTPSDSWAPYVELSANYKINKDWDAFFVGRYVQLSDEVKDSPMVDK
ECCCCCHHHCCCCCCCCCCCCCCEEEEECCEEECCCCCEEEEEEEEEECCCCCCCCCCCC
SYTGLLMTGVKYSF
CCCCEEEECEEECC
>Mature Secondary Structure
MEKEPVKIFKLKTLAIIASAMVCAQSAQAGTWSLGASALVSPDPYRGYQDRVYPVPVINY
CCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCCCCCCCCCCEEEEEEEEC
EGDDFYFRTLTAGYYLWKDQQNQLSLMGYFSPLGYRPGDSDDDRMKQLNKRRGTLMAGLA
CCCCEEEEEEECEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEHHH
YSHFAEWGILRTTFTGDTLDYSNGMVGDLAYLYKFDLGDFTLVPGVGVSWSSKNQNKYYY
HHHHHHCCEEEEEECCCCEECCCCCHHHEEEEEEECCCCEEECCCCCCEECCCCCCEEEE
GISENESRRSGLDSYTPSDSWAPYVELSANYKINKDWDAFFVGRYVQLSDEVKDSPMVDK
ECCCCCHHHCCCCCCCCCCCCCCEEEEECCEEECCCCCEEEEEEEEEECCCCCCCCCCCC
SYTGLLMTGVKYSF
CCCCEEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 10037771; 10806384 [H]