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The map label for this gene is rihB [H]

Identifier: 157370920

GI number: 157370920

Start: 2949950

End: 2950918

Strand: Reverse

Name: rihB [H]

Synonym: Spro_2680

Alternate gene names: 157370920

Gene position: 2950918-2949950 (Counterclockwise)

Preceding gene: 157370921

Following gene: 157370916

Centisome position: 54.16

GC content: 53.35

Gene sequence:

>969_bases
ATGAAGAGAAAAGTCATTTTTGATACGGATCCCGGTATTGATGACACTATGGCGATGCTATTGGCGCATGCCAGTGACCA
GATTGAGCTGGTGGGCATTACCACGGTTTTTGGCAATGCGACCATTGAGAATGCCACAAGAAACGCCCTGTATATAAAGC
AGCGGTTTGGCCTGACTGCTGATGTCGCCGTGGGCGCGGATACGCCTTTGGTGGTAGCGGCGGGTGAGGCGACCACTTTC
GTTCACGGCGAAAATGGTTTGGGGGATATAGATATCCCCGCGGGGGATTACCAGGGTATTGATAAGCGAGCGGCACATGA
CTACATCATCGATAGCGTAAAAGCGCATCCCGGTGAAATCACGCTGATTGCCGTCGGCAGGTTGACCAATCTTGCCCTGG
CGGTTGAAAAGGATCCCGGTATCGCCAGCCTGGTTAAAGAGGTGATTATTATGGGCGGGGCATTTGGCCATCACGGGCAT
ACGGGCAATGTTACCCCTTTTGCCGAGGCCAATATTATCGGTGATCCGCATGCTGCCGATAGGGTGATGACCACCGACTG
GCCGGTTACCGTCGTCGGACTGGATGTCACTCAGCAAACGGTGATGAGCAGCGAATATATTGAGCGGTTGCGCATCAATT
CAGCCCGGTACGGTGAGTTCATTTACCAGATCACGCGTTTTTACGCTGATTTTCATAAGAGGGAGATCGGCATGGATGGA
TTCTATGTACATGACTCCTCGGCCATTGCCTATGCCATTGCGCCCGAGTTGTTTGACGTAAAAGTCGGTGCTGTACGGGT
GATTACCGAGGGGCCGGCCATCGGGCATACCCTGCTTAAGGAAGGGGCGAAACCTTACCCTATCGATGAGTGGAGTGGCA
AACCTCAGCAACGGGTCTGTGTTCAGGTTGACCATCGGGGCGTGCTTGATCTCTATATGGCGACCATGGCGGAGAGGGTT
ACCGCTTAG

Upstream 100 bases:

>100_bases
TCATGACCGCCCTGGGTTTTACTGCCGTATGCGTTTGCGTTGTCGCCTGCCTGATCCTTGAGAAGGTTGAAAATTCCAAA
TCCACAAAGGAAATATACTT

Downstream 100 bases:

>100_bases
ACTACGCGTTTTTGATGGTCATTTGCCAAAATCGGTGGGTTCACTGACTGAGCCCCTGACAACCAGGCTGGGCGTGGCGG
ATATTATTGCTTTACTGCCT

Product: inosine/uridine-preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKRKVIFDTDPGIDDTMAMLLAHASDQIELVGITTVFGNATIENATRNALYIKQRFGLTADVAVGADTPLVVAAGEATTF
VHGENGLGDIDIPAGDYQGIDKRAAHDYIIDSVKAHPGEITLIAVGRLTNLALAVEKDPGIASLVKEVIIMGGAFGHHGH
TGNVTPFAEANIIGDPHAADRVMTTDWPVTVVGLDVTQQTVMSSEYIERLRINSARYGEFIYQITRFYADFHKREIGMDG
FYVHDSSAIAYAIAPELFDVKVGAVRVITEGPAIGHTLLKEGAKPYPIDEWSGKPQQRVCVQVDHRGVLDLYMATMAERV
TA

Sequences:

>Translated_322_residues
MKRKVIFDTDPGIDDTMAMLLAHASDQIELVGITTVFGNATIENATRNALYIKQRFGLTADVAVGADTPLVVAAGEATTF
VHGENGLGDIDIPAGDYQGIDKRAAHDYIIDSVKAHPGEITLIAVGRLTNLALAVEKDPGIASLVKEVIIMGGAFGHHGH
TGNVTPFAEANIIGDPHAADRVMTTDWPVTVVGLDVTQQTVMSSEYIERLRINSARYGEFIYQITRFYADFHKREIGMDG
FYVHDSSAIAYAIAPELFDVKVGAVRVITEGPAIGHTLLKEGAKPYPIDEWSGKPQQRVCVQVDHRGVLDLYMATMAERV
TA
>Mature_322_residues
MKRKVIFDTDPGIDDTMAMLLAHASDQIELVGITTVFGNATIENATRNALYIKQRFGLTADVAVGADTPLVVAAGEATTF
VHGENGLGDIDIPAGDYQGIDKRAAHDYIIDSVKAHPGEITLIAVGRLTNLALAVEKDPGIASLVKEVIIMGGAFGHHGH
TGNVTPFAEANIIGDPHAADRVMTTDWPVTVVGLDVTQQTVMSSEYIERLRINSARYGEFIYQITRFYADFHKREIGMDG
FYVHDSSAIAYAIAPELFDVKVGAVRVITEGPAIGHTLLKEGAKPYPIDEWSGKPQQRVCVQVDHRGVLDLYMATMAERV
TA

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=285, Percent_Identity=36.8421052631579, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1786213, Length=310, Percent_Identity=34.1935483870968, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1786871, Length=317, Percent_Identity=33.1230283911672, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32565474, Length=190, Percent_Identity=32.6315789473684, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320608, Length=259, Percent_Identity=30.5019305019305, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641839, Length=191, Percent_Identity=31.9371727748691, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24641837, Length=227, Percent_Identity=31.7180616740088, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 34860; Mature: 34860

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKVIFDTDPGIDDTMAMLLAHASDQIELVGITTVFGNATIENATRNALYIKQRFGLTA
CCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCEECCCCCCEEEEEECCCCEE
DVAVGADTPLVVAAGEATTFVHGENGLGDIDIPAGDYQGIDKRAAHDYIIDSVKAHPGEI
EEEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCE
TLIAVGRLTNLALAVEKDPGIASLVKEVIIMGGAFGHHGHTGNVTPFAEANIIGDPHAAD
EEEEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RVMTTDWPVTVVGLDVTQQTVMSSEYIERLRINSARYGEFIYQITRFYADFHKREIGMDG
CEEECCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
FYVHDSSAIAYAIAPELFDVKVGAVRVITEGPAIGHTLLKEGAKPYPIDEWSGKPQQRVC
EEEECCCEEEEEECCCCEEEEECEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCEEE
VQVDHRGVLDLYMATMAERVTA
EEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRKVIFDTDPGIDDTMAMLLAHASDQIELVGITTVFGNATIENATRNALYIKQRFGLTA
CCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCEECCCCCCEEEEEECCCCEE
DVAVGADTPLVVAAGEATTFVHGENGLGDIDIPAGDYQGIDKRAAHDYIIDSVKAHPGEI
EEEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCE
TLIAVGRLTNLALAVEKDPGIASLVKEVIIMGGAFGHHGHTGNVTPFAEANIIGDPHAAD
EEEEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RVMTTDWPVTVVGLDVTQQTVMSSEYIERLRINSARYGEFIYQITRFYADFHKREIGMDG
CEEECCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
FYVHDSSAIAYAIAPELFDVKVGAVRVITEGPAIGHTLLKEGAKPYPIDEWSGKPQQRVC
EEEECCCEEEEEECCCCEEEEECEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCEEE
VQVDHRGVLDLYMATMAERVTA
EEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA