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The map label for this gene is dacD [H]

Identifier: 157370876

GI number: 157370876

Start: 2900583

End: 2901752

Strand: Reverse

Name: dacD [H]

Synonym: Spro_2636

Alternate gene names: 157370876

Gene position: 2901752-2900583 (Counterclockwise)

Preceding gene: 157370880

Following gene: 157370875

Centisome position: 53.25

GC content: 56.41

Gene sequence:

>1170_bases
TTGAAGCGTTCAGTATTGGCGGTGATCACCGGAATTTTGCTGCCGTTGGCGGTACAGGCGGCAGATGCGCCACTCAACTT
TCCCCTTAATACCCCGCCGGCGATAGACGCCGCCTCTTACGTGCTGATGGATTACACCACTGGCCAGGTACTGGCACAGG
AAAATGCCGATCAGAGGCGTAATCCGGCCAGCCTGACCAAACTGATGACCGGGTTGGTGATTGATCACGCGCTCGATCAA
CACAAGATTGGTCTGGATGACGTGGTGACGGTGGGTAAAGATGCCTGGGCGCAGGGCAATCCGGTGTTCAAAGGCTCTTC
GTTGATGTTCCTGAAGCCCGGTGACAGGGTCACGGTTCGCGATCTCAGCCGCGGCATTATCATTGACTCCGGCAACGACG
CCTGCGTAGCCATGGCGGACTACGTGGCGGGCAGTCAGGCCGCATTCGTCAAACTGATGAATGAAAAGAGCGCCCAGCTT
GGCCTGCAAAACACCCATTTTGAAACCGTCCACGGTCTGGATGCCCCAGGGCAATTCACCACGGCGGGCGATCTGGCGGT
GATCTCCCGCGCCATTATTATGAGCGAACCGGCGGAATACCATATGTACAGCGAGAAATCGCTGACCTGGAACGGTATCA
CCCAGCAGAACCGCAACGGGCTGTTGTGGGACAAAAACCTGCGGGTGGACGGCCTGAAAACCGGCCATACCGAATCCGCC
GGTTTTAACATTATTGCGTCTGCCACCGAAGGCGACCGTCGGCTGATTGCCGTAGTGATGGGCGGTAAAAGTCCAAAAGG
GCGCGAAGAACAGGCGCGTAAACTGCTGACCTGGGGGCTGCGTGATTTCGTTACCCTACATTTGTTTAGCGCTGGGCAAA
GCCTGGGGCAGGAGAAAGTGTGGTACGGCGATCGCCATGAGGTTGCGGTGGGCAGCCAACAGGATCAATATCTCAGCCTG
CCGAAAAGCGAGGCGGATAAGCTGAAGGCGCAATATGTGATCAATACGCCGCGTCTTGATGCTCCGCTGACTCAGGGGCA
GTCGGTCGGCGAAATTCGTATCAGTGACAATGGCAAAGTACTGAAAACCTTGCCGCTGGTGGTGTTGCAGCCGGTCAATC
AGGGCGGCATGTTCTCGCGCCTGATGGACTATGTGAAGTTGAAAATCTGA

Upstream 100 bases:

>100_bases
TGAAAAAGAGTAATGATTCCGCTTTCGCCACAATTAATGCAGTCTATGCCTCGGATTATATTGGTATTTTATTTTGCCCG
CAGGGCCTGAGGAACAGCAA

Downstream 100 bases:

>100_bases
CTCTCGGGGCGCAGCGCTCCGCGCCCGAAATCCAGCAGCGATGATATACTCAGGTCAGTCATGACTTGTTGAGCCGTTTT
ATGGATATTAAGCAACTGAT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKRSVLAVITGILLPLAVQAADAPLNFPLNTPPAIDAASYVLMDYTTGQVLAQENADQRRNPASLTKLMTGLVIDHALDQ
HKIGLDDVVTVGKDAWAQGNPVFKGSSLMFLKPGDRVTVRDLSRGIIIDSGNDACVAMADYVAGSQAAFVKLMNEKSAQL
GLQNTHFETVHGLDAPGQFTTAGDLAVISRAIIMSEPAEYHMYSEKSLTWNGITQQNRNGLLWDKNLRVDGLKTGHTESA
GFNIIASATEGDRRLIAVVMGGKSPKGREEQARKLLTWGLRDFVTLHLFSAGQSLGQEKVWYGDRHEVAVGSQQDQYLSL
PKSEADKLKAQYVINTPRLDAPLTQGQSVGEIRISDNGKVLKTLPLVVLQPVNQGGMFSRLMDYVKLKI

Sequences:

>Translated_389_residues
MKRSVLAVITGILLPLAVQAADAPLNFPLNTPPAIDAASYVLMDYTTGQVLAQENADQRRNPASLTKLMTGLVIDHALDQ
HKIGLDDVVTVGKDAWAQGNPVFKGSSLMFLKPGDRVTVRDLSRGIIIDSGNDACVAMADYVAGSQAAFVKLMNEKSAQL
GLQNTHFETVHGLDAPGQFTTAGDLAVISRAIIMSEPAEYHMYSEKSLTWNGITQQNRNGLLWDKNLRVDGLKTGHTESA
GFNIIASATEGDRRLIAVVMGGKSPKGREEQARKLLTWGLRDFVTLHLFSAGQSLGQEKVWYGDRHEVAVGSQQDQYLSL
PKSEADKLKAQYVINTPRLDAPLTQGQSVGEIRISDNGKVLKTLPLVVLQPVNQGGMFSRLMDYVKLKI
>Mature_389_residues
MKRSVLAVITGILLPLAVQAADAPLNFPLNTPPAIDAASYVLMDYTTGQVLAQENADQRRNPASLTKLMTGLVIDHALDQ
HKIGLDDVVTVGKDAWAQGNPVFKGSSLMFLKPGDRVTVRDLSRGIIIDSGNDACVAMADYVAGSQAAFVKLMNEKSAQL
GLQNTHFETVHGLDAPGQFTTAGDLAVISRAIIMSEPAEYHMYSEKSLTWNGITQQNRNGLLWDKNLRVDGLKTGHTESA
GFNIIASATEGDRRLIAVVMGGKSPKGREEQARKLLTWGLRDFVTLHLFSAGQSLGQEKVWYGDRHEVAVGSQQDQYLSL
PKSEADKLKAQYVINTPRLDAPLTQGQSVGEIRISDNGKVLKTLPLVVLQPVNQGGMFSRLMDYVKLKI

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=389, Percent_Identity=59.6401028277635, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1786851, Length=389, Percent_Identity=53.7275064267352, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1787062, Length=357, Percent_Identity=52.1008403361345, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI87082054, Length=285, Percent_Identity=25.2631578947368, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42306; Mature: 42306

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSVLAVITGILLPLAVQAADAPLNFPLNTPPAIDAASYVLMDYTTGQVLAQENADQRR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCHHCC
NPASLTKLMTGLVIDHALDQHKIGLDDVVTVGKDAWAQGNPVFKGSSLMFLKPGDRVTVR
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEECCCEEEEECCCCEEEEE
DLSRGIIIDSGNDACVAMADYVAGSQAAFVKLMNEKSAQLGLQNTHFETVHGLDAPGQFT
ECCCCEEEECCCCCEEEEEHHHCCCHHHHHHHHHCHHHHCCCCCCCCHHCCCCCCCCCCC
TAGDLAVISRAIIMSEPAEYHMYSEKSLTWNGITQQNRNGLLWDKNLRVDGLKTGHTESA
CCCHHHHHHHHHHHCCCHHHEEECCCCCEECCCCCCCCCCCEEECCCEECCCCCCCCCCC
GFNIIASATEGDRRLIAVVMGGKSPKGREEQARKLLTWGLRDFVTLHLFSAGQSLGQEKV
CCEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
WYGDRHEVAVGSQQDQYLSLPKSEADKLKAQYVINTPRLDAPLTQGQSVGEIRISDNGKV
EECCCCEEEECCCCCHHCCCCCCHHHHEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCE
LKTLPLVVLQPVNQGGMFSRLMDYVKLKI
EEEECEEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKRSVLAVITGILLPLAVQAADAPLNFPLNTPPAIDAASYVLMDYTTGQVLAQENADQRR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCHHCC
NPASLTKLMTGLVIDHALDQHKIGLDDVVTVGKDAWAQGNPVFKGSSLMFLKPGDRVTVR
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEECCCEEEEECCCCEEEEE
DLSRGIIIDSGNDACVAMADYVAGSQAAFVKLMNEKSAQLGLQNTHFETVHGLDAPGQFT
ECCCCEEEECCCCCEEEEEHHHCCCHHHHHHHHHCHHHHCCCCCCCCHHCCCCCCCCCCC
TAGDLAVISRAIIMSEPAEYHMYSEKSLTWNGITQQNRNGLLWDKNLRVDGLKTGHTESA
CCCHHHHHHHHHHHCCCHHHEEECCCCCEECCCCCCCCCCCEEECCCEECCCCCCCCCCC
GFNIIASATEGDRRLIAVVMGGKSPKGREEQARKLLTWGLRDFVTLHLFSAGQSLGQEKV
CCEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
WYGDRHEVAVGSQQDQYLSLPKSEADKLKAQYVINTPRLDAPLTQGQSVGEIRISDNGKV
EECCCCEEEECCCCCHHCCCCCCHHHHEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCE
LKTLPLVVLQPVNQGGMFSRLMDYVKLKI
EEEECEEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]