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The map label for this gene is pntA [H]

Identifier: 157370825

GI number: 157370825

Start: 2841919

End: 2843448

Strand: Reverse

Name: pntA [H]

Synonym: Spro_2585

Alternate gene names: 157370825

Gene position: 2843448-2841919 (Counterclockwise)

Preceding gene: 157370828

Following gene: 157370824

Centisome position: 52.18

GC content: 57.65

Gene sequence:

>1530_bases
ATGCGTATTGGTGTACCAAGAGAACGGTTGGCCAATGAAGCCCGGGTCGCAGCAACGCCGAAAACGGTGGAACAACTGCT
GAAGCTGGGCTTTACCGTCGCGATTGAAAGCGGGGCGGGTAAACTGGCAAGTTTTGACGATAGCGCTTATGAAGCCGCTG
GGGCAACAATCACTGACACCGCGGATGTCTGGCAGTCAGACCTGATTTTGAAAGTTAACGCACCGCTTGAGGACGAGATC
GCGTTAATGCGCGAGGGCAGCACCCTGGTCAGCTTTATCTGGCCGGCGCAAAACCCGGAGCTGATCGCGCAGCTGGCCGC
TCGCAACGTCACTGCGATGGCTATGGATTCTGTACCGCGTATCTCACGCGCACAATCCATGGACGCACTGAGCTCAATGG
CCAACATCGCCGGTTACCGTGCGATCGTTGAGGCCGCGCATGAGTTTGGCCGCTTCTTCACCGGGCAAATCACCGCTGCC
GGCAAGGTTCCACCCGCCAAGGTAATGATTATCGGTGCCGGCGTAGCGGGCCTGGCGGCCATCGGCGCCGCAGGCAGCCT
GGGGGCGATTGTTCGCGCCTTCGACACCCGCCCGGAAGTCAAAGAGCAGGTGCAGAGCATGGGCGCTGAATTCCTCGAGC
TGGATTTTGAGGAAGAAGCGGGCAGCGGCGATGGCTATGCCAAAGTGATGTCCGAAGCCTTTATCAAGGCCGAGATGGCG
CTGTTCGCCGCCCAGGCGGCCGAGGTCGACATTATTGTCACCACCGCGTTGATCCCGGGCAAACCCGCACCGAAGCTTAT
CACCAAAGAGATGGTGGCTTCGATGAAACCGGGTAGCGTGATCGTCGATCTGGCGGCGCAAAACGGTGGCAACTGTGAAC
TGACCGTGGCCGATCGCGTGACCGTGACCGACAACGGCGTGAAAATCATTGGCTATACCGATTTGCCAAGCCGCCTGCCG
ACTCAATCCTCACAGCTTTACGGCACCAACCTGGTTAACCTGCTGAAATTGCTGTCGAAAGAGAAAAACGGCGAAATTGA
CGTTGATTTTGAAGATACCGTGATCCGCGGCGTTACCGTGGTGCGCAGTGGTGAAGTCACCTGGCCGGCACCGCCAATCC
AGGTTTCTGCCCAGCCTAAACAGGCACCGGCAGCGGCACCGGTCGCCAAACCGGCGGCAAAACCGGTTTCGCCATGGTTG
AAATATGGCCTGATGGCGCTGGCCATCATCCTGTTTGGCTGGCTGGCCAACGCCGCACCAAAAGAGTTCCTGTCTCACTT
TACCGTGTTTGCGCTGGCCTGCGTGGTGGGTTACTACGTGGTTTGGAACGTCAGCCATGCATTACATACCCCGTTGATGT
CGGTCACCAATGCGATCTCAGGGATTATCGTGGTCGGTGCGCTATTGCAGATTGGCCATGGCGGCTGGGTGAGTTTCCTC
TCCTTTATCGCCGTGCTGATCGCCAGCATCAACATTTTTGGTGGATTCACCGTCACTCAGCGCATGCTGAAGATGTTCCG
CAAGAACTAA

Upstream 100 bases:

>100_bases
ATTCGTTGCCGACAAGGCTAATAAAAACCTGTCGGCCGGGGCCATCGCTATGGGTAGGAAGATAATAAACATCATCATAG
ACAGGTGAGAAGGGAACATT

Downstream 100 bases:

>100_bases
GGGGTAGCATATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCTATCCTGTTTATCTGTAGTCTGGCCGGCC
TGTCGCGCCATGAAACGTCC

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGKLASFDDSAYEAAGATITDTADVWQSDLILKVNAPLEDEI
ALMREGSTLVSFIWPAQNPELIAQLAARNVTAMAMDSVPRISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMIIGAGVAGLAAIGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
LFAAQAAEVDIIVTTALIPGKPAPKLITKEMVASMKPGSVIVDLAAQNGGNCELTVADRVTVTDNGVKIIGYTDLPSRLP
TQSSQLYGTNLVNLLKLLSKEKNGEIDVDFEDTVIRGVTVVRSGEVTWPAPPIQVSAQPKQAPAAAPVAKPAAKPVSPWL
KYGLMALAIILFGWLANAAPKEFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFL
SFIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_509_residues
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGKLASFDDSAYEAAGATITDTADVWQSDLILKVNAPLEDEI
ALMREGSTLVSFIWPAQNPELIAQLAARNVTAMAMDSVPRISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMIIGAGVAGLAAIGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
LFAAQAAEVDIIVTTALIPGKPAPKLITKEMVASMKPGSVIVDLAAQNGGNCELTVADRVTVTDNGVKIIGYTDLPSRLP
TQSSQLYGTNLVNLLKLLSKEKNGEIDVDFEDTVIRGVTVVRSGEVTWPAPPIQVSAQPKQAPAAAPVAKPAAKPVSPWL
KYGLMALAIILFGWLANAAPKEFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFL
SFIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_509_residues
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGKLASFDDSAYEAAGATITDTADVWQSDLILKVNAPLEDEI
ALMREGSTLVSFIWPAQNPELIAQLAARNVTAMAMDSVPRISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMIIGAGVAGLAAIGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
LFAAQAAEVDIIVTTALIPGKPAPKLITKEMVASMKPGSVIVDLAAQNGGNCELTVADRVTVTDNGVKIIGYTDLPSRLP
TQSSQLYGTNLVNLLKLLSKEKNGEIDVDFEDTVIRGVTVVRSGEVTWPAPPIQVSAQPKQAPAAAPVAKPAAKPVSPWL
KYGLMALAIILFGWLANAAPKEFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFL
SFIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=546, Percent_Identity=49.4505494505495, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI122939153, Length=546, Percent_Identity=49.4505494505495, Blast_Score=484, Evalue=1e-136,
Organism=Escherichia coli, GI1787887, Length=510, Percent_Identity=85.2941176470588, Blast_Score=868, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=519, Percent_Identity=50.6743737957611, Blast_Score=477, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 53882; Mature: 53882

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGKLASFDDSAYEAAGATITDT
CCCCCCHHHHCCCHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHCCCEECCC
ADVWQSDLILKVNAPLEDEIALMREGSTLVSFIWPAQNPELIAQLAARNVTAMAMDSVPR
HHHHCCCEEEEECCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCH
ISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMIIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
HCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHEECCHHCCCCCCHHHHHHHHHHHHHHHH
LFAAQAAEVDIIVTTALIPGKPAPKLITKEMVASMKPGSVIVDLAAQNGGNCELTVADRV
HHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECEE
TVTDNGVKIIGYTDLPSRLPTQSSQLYGTNLVNLLKLLSKEKNGEIDVDFEDTVIRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCCEECCHHHHHHHCEEE
VRSGEVTWPAPPIQVSAQPKQAPAAAPVAKPAAKPVSPWLKYGLMALAIILFGWLANAAP
EECCCEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
KEFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGKLASFDDSAYEAAGATITDT
CCCCCCHHHHCCCHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHCCCEECCC
ADVWQSDLILKVNAPLEDEIALMREGSTLVSFIWPAQNPELIAQLAARNVTAMAMDSVPR
HHHHCCCEEEEECCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCH
ISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMIIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
HCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHEECCHHCCCCCCHHHHHHHHHHHHHHHH
LFAAQAAEVDIIVTTALIPGKPAPKLITKEMVASMKPGSVIVDLAAQNGGNCELTVADRV
HHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECEE
TVTDNGVKIIGYTDLPSRLPTQSSQLYGTNLVNLLKLLSKEKNGEIDVDFEDTVIRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCCEECCHHHHHHHCEEE
VRSGEVTWPAPPIQVSAQPKQAPAAAPVAKPAAKPVSPWLKYGLMALAIILFGWLANAAP
EECCCEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
KEFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]