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The map label for this gene is hypB [H]

Identifier: 157370676

GI number: 157370676

Start: 2669114

End: 2669908

Strand: Direct

Name: hypB [H]

Synonym: Spro_2436

Alternate gene names: 157370676

Gene position: 2669114-2669908 (Clockwise)

Preceding gene: 157370675

Following gene: 157370677

Centisome position: 48.98

GC content: 57.74

Gene sequence:

>795_bases
ATGTGCAGTACCTGCGGTTGTGCCCAGGGTGAATTAACCATTGAAGGGGATGTGAAGGTGCCATTCCGTCCGCAACGCAA
TGTGCAGGGCGATCTGCATTACGGTTTGGGGCTGGCAGGCACCCATGCACCGGGTATCAGCCAGCGCCGGATGCTGGAAA
TTGAAACCCGGGTGCTGGATAAGAACGATCGTCTGGCGGAGGGTAATCGCCAGTATTTCGCCGACCAACATATTCTGGCG
CTGAATCTGGTTTCCAGCCCCGGCTCCGGCAAAACCACGCTGCTGACAGAAACGCTGCTGCGGCTGCGGGGTCAGCACCC
GTGCGCGGTGGTGGAAGGCGATCAGCAAACCACCAATGACGCGGAACGTATCCGCGCCACCGGTGTACCGGCTATTCAGG
TCAATACCGGTAAAGGCTGCCATCTGGATGCGCAAATGGTTCACGAGGCCGCCCGGCGTCTGGAACTCAAACCCCACAGC
CTGTTGTTTATCGAGAACGTCGGCAATCTGGTTTGCCCGGCCAGCTTTGATCTGGGGGAGCGCCACAAGGTAGCGGTGCT
GTCGGTTACCGAAGGGGAAGACAAACCCCTCAAGTATCCACATATGTTTGCCGCCGCACGGGTGATGCTGCTGAACAAAA
TCGATCTGCTGCCGTATTTGAACTTCGACCTGGCAGCCTGTATCGACAATGCCCGCAAGGTGAACCCGACGATTGAGGTG
ATTACCCTCTCGGCGACCAGCGGTGAAGGTATGGATCAGTGGCTGGCCTGGCTGGAGACACAGTTATGTGCATAG

Upstream 100 bases:

>100_bases
GCTGGTCTGTCCGCACTGTACCGGACGAAATCTGCGGGTACAGGCCGACGATGGCTTGCAAATAAAACGCCTGGAAATTG
AATAACAGAGGGGAATGAAT

Downstream 100 bases:

>100_bases
GCGTTCCAGGGCAGATCGTGGCGCTGGATGAAAAGCAGCCTCAGCATGCCTGGGCCGAAGTCTGTGGTGTGCAGCGGGAA
GTGAATATTGCGCTGGTGCG

Product: hydrogenase nickel incorporation protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MCSTCGCAQGELTIEGDVKVPFRPQRNVQGDLHYGLGLAGTHAPGISQRRMLEIETRVLDKNDRLAEGNRQYFADQHILA
LNLVSSPGSGKTTLLTETLLRLRGQHPCAVVEGDQQTTNDAERIRATGVPAIQVNTGKGCHLDAQMVHEAARRLELKPHS
LLFIENVGNLVCPASFDLGERHKVAVLSVTEGEDKPLKYPHMFAAARVMLLNKIDLLPYLNFDLAACIDNARKVNPTIEV
ITLSATSGEGMDQWLAWLETQLCA

Sequences:

>Translated_264_residues
MCSTCGCAQGELTIEGDVKVPFRPQRNVQGDLHYGLGLAGTHAPGISQRRMLEIETRVLDKNDRLAEGNRQYFADQHILA
LNLVSSPGSGKTTLLTETLLRLRGQHPCAVVEGDQQTTNDAERIRATGVPAIQVNTGKGCHLDAQMVHEAARRLELKPHS
LLFIENVGNLVCPASFDLGERHKVAVLSVTEGEDKPLKYPHMFAAARVMLLNKIDLLPYLNFDLAACIDNARKVNPTIEV
ITLSATSGEGMDQWLAWLETQLCA
>Mature_264_residues
MCSTCGCAQGELTIEGDVKVPFRPQRNVQGDLHYGLGLAGTHAPGISQRRMLEIETRVLDKNDRLAEGNRQYFADQHILA
LNLVSSPGSGKTTLLTETLLRLRGQHPCAVVEGDQQTTNDAERIRATGVPAIQVNTGKGCHLDAQMVHEAARRLELKPHS
LLFIENVGNLVCPASFDLGERHKVAVLSVTEGEDKPLKYPHMFAAARVMLLNKIDLLPYLNFDLAACIDNARKVNPTIEV
ITLSATSGEGMDQWLAWLETQLCA

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=290, Percent_Identity=69.3103448275862, Blast_Score=403, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 28931; Mature: 28931

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSTCGCAQGELTIEGDVKVPFRPQRNVQGDLHYGLGLAGTHAPGISQRRMLEIETRVLD
CCCCCCCCCCCEEEECCEECCCCCCCCCCCCEEECCCCCCCCCCCCCCHHEEEEHHHHCC
KNDRLAEGNRQYFADQHILALNLVSSPGSGKTTLLTETLLRLRGQHPCAVVEGDQQTTND
CCCCCCCCCCEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCH
AERIRATGVPAIQVNTGKGCHLDAQMVHEAARRLELKPHSLLFIENVGNLVCPASFDLGE
HHHHHHCCCCEEEEECCCCCEECHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCCCCC
RHKVAVLSVTEGEDKPLKYPHMFAAARVMLLNKIDLLPYLNFDLAACIDNARKVNPTIEV
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEE
ITLSATSGEGMDQWLAWLETQLCA
EEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MCSTCGCAQGELTIEGDVKVPFRPQRNVQGDLHYGLGLAGTHAPGISQRRMLEIETRVLD
CCCCCCCCCCCEEEECCEECCCCCCCCCCCCEEECCCCCCCCCCCCCCHHEEEEHHHHCC
KNDRLAEGNRQYFADQHILALNLVSSPGSGKTTLLTETLLRLRGQHPCAVVEGDQQTTND
CCCCCCCCCCEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCH
AERIRATGVPAIQVNTGKGCHLDAQMVHEAARRLELKPHSLLFIENVGNLVCPASFDLGE
HHHHHHCCCCEEEEECCCCCEECHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCCCCC
RHKVAVLSVTEGEDKPLKYPHMFAAARVMLLNKIDLLPYLNFDLAACIDNARKVNPTIEV
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEE
ITLSATSGEGMDQWLAWLETQLCA
EEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]