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The map label for this gene is tcyA [H]

Identifier: 157370603

GI number: 157370603

Start: 2577169

End: 2578083

Strand: Reverse

Name: tcyA [H]

Synonym: Spro_2363

Alternate gene names: 157370603

Gene position: 2578083-2577169 (Counterclockwise)

Preceding gene: 157370604

Following gene: 157370602

Centisome position: 47.31

GC content: 58.25

Gene sequence:

>915_bases
ATGAAAATCACCATTAAACGCACCGCTCTGTCCCTGCTGCTGGCCTCCGCTGCACTGGCACCGTTGGCCAGCGGTTTCGC
CGCCGAAGGCGATGTGCCCTTCCGTGCCGCCGTGAAAGCCAGCGCCGATCCTGCGCTGCACGACAGCCTGCCTGAAGCCA
TCAAAAAGACCGGTTACATTGTGGCGGGCACCAACCCGAATACCCCACCCACCACCTTCTACGAAGCGGATAACAAAACG
CTGGCCGGTCGTGAAATTGATGTGATGAGCGCGATTGCCGATCGTCTGGGCGTGGCCATTCACTGGAAAGATACCGGCGG
TTTCGACAATATCATTCCCGGCCTGAAATCCGGCCGCTATGACGTGGCGCTGGCCAATATCGATGCCAACAAAAAACGCT
TCCAACAGGTTGATTTTGTCGGCTATTACAACGCCTCCAAACTGGCACTGATTGCCCGTAAAGATGCGGCACTGGGGCCA
TATACCGATCTGGCGCAGCTTTGTGGCCAGACCGTCGGTGCGGGTGCAGGCACTTCGCAAATCACCCGTTTGCAGCAGGC
CAGCGAACAATGCGTCAGCGGCGGCAAACCGGCCATTACCATTCCGATTTTCCCAGACCGCCCGGCCGGGGTTCAGGCGG
TGATCAGCGGCCGGGTGCCGATGTTCTTCGGCCCTTATGAAGGCTTGCGTTACCAGGCGACCCACGTTAAGCCGCTGGCG
CTGGCCGGTGATATCAACATTGAAGGCACTACGGTGGCCATCGCCTTGCCGAAAGGCTCCGAGCTGGTGAAACCGGTACA
GGCGGCGCTGAACTCGCTGATTGCCGATGGCAGCTATCAGAAAATCCTCGACGGCTGGGATATCGGTTTTGGCGCGGTGA
AAACCGCCGGTATTAATGAAGAGATCGCAAAATGA

Upstream 100 bases:

>100_bases
GAAGAATATCAGTATGCAACCCTGCGTGAAAACCTGGGCCTGCCGCAACCGGCAATCAACTTCGCCAACGTTAAATCGGC
TTAATTCGCAGGGGAACATC

Downstream 100 bases:

>100_bases
GTGGGCAATACCCTACCGACGATGCCGAGCTGCGCATTGTCGGTCATCGGCATTACGGCCGCTGGTTCAGCGCACTGCTG
GTGCTGTTGATCGTTGGCAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKITIKRTALSLLLASAALAPLASGFAAEGDVPFRAAVKASADPALHDSLPEAIKKTGYIVAGTNPNTPPTTFYEADNKT
LAGREIDVMSAIADRLGVAIHWKDTGGFDNIIPGLKSGRYDVALANIDANKKRFQQVDFVGYYNASKLALIARKDAALGP
YTDLAQLCGQTVGAGAGTSQITRLQQASEQCVSGGKPAITIPIFPDRPAGVQAVISGRVPMFFGPYEGLRYQATHVKPLA
LAGDINIEGTTVAIALPKGSELVKPVQAALNSLIADGSYQKILDGWDIGFGAVKTAGINEEIAK

Sequences:

>Translated_304_residues
MKITIKRTALSLLLASAALAPLASGFAAEGDVPFRAAVKASADPALHDSLPEAIKKTGYIVAGTNPNTPPTTFYEADNKT
LAGREIDVMSAIADRLGVAIHWKDTGGFDNIIPGLKSGRYDVALANIDANKKRFQQVDFVGYYNASKLALIARKDAALGP
YTDLAQLCGQTVGAGAGTSQITRLQQASEQCVSGGKPAITIPIFPDRPAGVQAVISGRVPMFFGPYEGLRYQATHVKPLA
LAGDINIEGTTVAIALPKGSELVKPVQAALNSLIADGSYQKILDGWDIGFGAVKTAGINEEIAK
>Mature_304_residues
MKITIKRTALSLLLASAALAPLASGFAAEGDVPFRAAVKASADPALHDSLPEAIKKTGYIVAGTNPNTPPTTFYEADNKT
LAGREIDVMSAIADRLGVAIHWKDTGGFDNIIPGLKSGRYDVALANIDANKKRFQQVDFVGYYNASKLALIARKDAALGP
YTDLAQLCGQTVGAGAGTSQITRLQQASEQCVSGGKPAITIPIFPDRPAGVQAVISGRVPMFFGPYEGLRYQATHVKPLA
LAGDINIEGTTVAIALPKGSELVKPVQAALNSLIADGSYQKILDGWDIGFGAVKTAGINEEIAK

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=229, Percent_Identity=25.764192139738, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788228, Length=258, Percent_Identity=27.1317829457364, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787088, Length=233, Percent_Identity=26.1802575107296, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31835; Mature: 31835

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITIKRTALSLLLASAALAPLASGFAAEGDVPFRAAVKASADPALHDSLPEAIKKTGYI
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCEE
VAGTNPNTPPTTFYEADNKTLAGREIDVMSAIADRLGVAIHWKDTGGFDNIIPGLKSGRY
EECCCCCCCCCEEEECCCCEECCCCHHHHHHHHHHHCEEEEEECCCCHHHHCCCCCCCCE
DVALANIDANKKRFQQVDFVGYYNASKLALIARKDAALGPYTDLAQLCGQTVGAGAGTSQ
EEEEEECCCCHHHHHHEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHH
ITRLQQASEQCVSGGKPAITIPIFPDRPAGVQAVISGRVPMFFGPYEGLRYQATHVKPLA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCEEECCCCCCEEEECCCCEEE
LAGDINIEGTTVAIALPKGSELVKPVQAALNSLIADGSYQKILDGWDIGFGAVKTAGINE
EECCCEECCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEECCCCH
EIAK
HHCC
>Mature Secondary Structure
MKITIKRTALSLLLASAALAPLASGFAAEGDVPFRAAVKASADPALHDSLPEAIKKTGYI
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCEE
VAGTNPNTPPTTFYEADNKTLAGREIDVMSAIADRLGVAIHWKDTGGFDNIIPGLKSGRY
EECCCCCCCCCEEEECCCCEECCCCHHHHHHHHHHHCEEEEEECCCCHHHHCCCCCCCCE
DVALANIDANKKRFQQVDFVGYYNASKLALIARKDAALGPYTDLAQLCGQTVGAGAGTSQ
EEEEEECCCCHHHHHHEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHH
ITRLQQASEQCVSGGKPAITIPIFPDRPAGVQAVISGRVPMFFGPYEGLRYQATHVKPLA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCEEECCCCCCEEEECCCCEEE
LAGDINIEGTTVAIALPKGSELVKPVQAALNSLIADGSYQKILDGWDIGFGAVKTAGINE
EECCCEECCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEECCCCH
EIAK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]