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The map label for this gene is ampR [H]

Identifier: 157370512

GI number: 157370512

Start: 2473107

End: 2473994

Strand: Reverse

Name: ampR [H]

Synonym: Spro_2272

Alternate gene names: 157370512

Gene position: 2473994-2473107 (Counterclockwise)

Preceding gene: 157370514

Following gene: 157370510

Centisome position: 45.4

GC content: 59.57

Gene sequence:

>888_bases
ATGGCTAACTTTATGCGTAACCGACTTCCCCTCAATGCCCTGCGGGCGTTTGAATCCTCCGCCCGTCACCTGAACTTTAC
CCGCGCCGGGCTGGAACTGAGCGTCACCCAGGCTGCCGTCAGCCAGCAGGTGCGTATGCTGGAACAGCAACTGGGTATTC
AGCTGTTCCGCCGTCTGCCGCGTGGATTGGATTTGACCGAAGAAGGACAGGCGCTGTTGCCGATATTGACCGACGCTTTC
GACCGTATTGAATCGGTGTTGCAACAGTTTGAAGGCGGGCATTTCCATGAGGTGCTGACGGTAGCCGTGGTCGGTACCTT
TGCCGTCGGCTGGCTGATGCCCCGTCTGGCGGCGTTCAGAGAGTCGCATCCGTTTATCGACCTGCGGGTGTTAACCAACA
ATAACCTGGTTAATTTGTCCGCCGACGGGATGGACTTCGCTATCCGTTTTGGCGAAGGCCGCTGGCCGGCGACCCACAAC
CTCAAATTGTTTGATGCCCCGCTGACGGTGCTCTGCCCGCCGGAGGTTGCCGCCCGCCTCCACAGTCCGCAGGATTTGCA
ACACGAGCGGCTGATGCGCAGCTACCGCAAGGACGAATGGGAACGCTGGTTTACCGCCGCACAGGTCACGCCGTGGCGGA
TTAACGGCCCGGTGTTCGACTCTTCCCGGCTGATGGTGGAAGGGGCGATCCACAGCGGTGGTGTAGCGCTGGCCCCGGCG
CGCATGTTTGCTCGGGAGCTGCGTGAAGGTTTGTTACAGCGCCCTTTCAGCGCCGAAGCGGATCTCGGCGGCTACTGGCT
GACCCACCTGAAATCACGCGATATGACCCCGGCAATGAAAGTGTTTGTCAGTTGGATCCAGCAACAGGTCACGGATGAAC
AACAATGA

Upstream 100 bases:

>100_bases
CTTTTCTGACTGCGTTAATTCCGCACCAGCAGGCTAAATATCAGGGTTTATCCCTGGGTTGACAAGCGATAAGAATTTCA
GCCAGCATAAAGAAAAACTT

Downstream 100 bases:

>100_bases
GGTCAGGCCGAGCCGCGCTTGATCAGCAATGGCGGCAGGATCACGTTTTGCGGCGGTAAGTCGGCGTCGGCAATCCGTTG
CAGCAAGCGTGAGGCGGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MANFMRNRLPLNALRAFESSARHLNFTRAGLELSVTQAAVSQQVRMLEQQLGIQLFRRLPRGLDLTEEGQALLPILTDAF
DRIESVLQQFEGGHFHEVLTVAVVGTFAVGWLMPRLAAFRESHPFIDLRVLTNNNLVNLSADGMDFAIRFGEGRWPATHN
LKLFDAPLTVLCPPEVAARLHSPQDLQHERLMRSYRKDEWERWFTAAQVTPWRINGPVFDSSRLMVEGAIHSGGVALAPA
RMFARELREGLLQRPFSAEADLGGYWLTHLKSRDMTPAMKVFVSWIQQQVTDEQQ

Sequences:

>Translated_295_residues
MANFMRNRLPLNALRAFESSARHLNFTRAGLELSVTQAAVSQQVRMLEQQLGIQLFRRLPRGLDLTEEGQALLPILTDAF
DRIESVLQQFEGGHFHEVLTVAVVGTFAVGWLMPRLAAFRESHPFIDLRVLTNNNLVNLSADGMDFAIRFGEGRWPATHN
LKLFDAPLTVLCPPEVAARLHSPQDLQHERLMRSYRKDEWERWFTAAQVTPWRINGPVFDSSRLMVEGAIHSGGVALAPA
RMFARELREGLLQRPFSAEADLGGYWLTHLKSRDMTPAMKVFVSWIQQQVTDEQQ
>Mature_294_residues
ANFMRNRLPLNALRAFESSARHLNFTRAGLELSVTQAAVSQQVRMLEQQLGIQLFRRLPRGLDLTEEGQALLPILTDAFD
RIESVLQQFEGGHFHEVLTVAVVGTFAVGWLMPRLAAFRESHPFIDLRVLTNNNLVNLSADGMDFAIRFGEGRWPATHNL
KLFDAPLTVLCPPEVAARLHSPQDLQHERLMRSYRKDEWERWFTAAQVTPWRINGPVFDSSRLMVEGAIHSGGVALAPAR
MFARELREGLLQRPFSAEADLGGYWLTHLKSRDMTPAMKVFVSWIQQQVTDEQQ

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=36.1204013377926, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=31.5972222222222, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788706, Length=308, Percent_Identity=32.7922077922078, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=25.9515570934256, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787128, Length=299, Percent_Identity=22.742474916388, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786401, Length=182, Percent_Identity=27.4725274725275, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI145693105, Length=218, Percent_Identity=26.605504587156, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693193, Length=143, Percent_Identity=30.0699300699301, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081978, Length=190, Percent_Identity=31.0526315789474, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=211, Percent_Identity=28.9099526066351, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33528; Mature: 33397

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFMRNRLPLNALRAFESSARHLNFTRAGLELSVTQAAVSQQVRMLEQQLGIQLFRRLP
CCCHHHHCCCHHHHHHHHHHHHHCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGLDLTEEGQALLPILTDAFDRIESVLQQFEGGHFHEVLTVAVVGTFAVGWLMPRLAAFR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ESHPFIDLRVLTNNNLVNLSADGMDFAIRFGEGRWPATHNLKLFDAPLTVLCPPEVAARL
CCCCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCEEEECCEEEECCHHHHHHC
HSPQDLQHERLMRSYRKDEWERWFTAAQVTPWRINGPVFDSSRLMVEGAIHSGGVALAPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEECCCCEEEEECCCCCCEEHHHH
RMFARELREGLLQRPFSAEADLGGYWLTHLKSRDMTPAMKVFVSWIQQQVTDEQQ
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ANFMRNRLPLNALRAFESSARHLNFTRAGLELSVTQAAVSQQVRMLEQQLGIQLFRRLP
CCHHHHCCCHHHHHHHHHHHHHCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGLDLTEEGQALLPILTDAFDRIESVLQQFEGGHFHEVLTVAVVGTFAVGWLMPRLAAFR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ESHPFIDLRVLTNNNLVNLSADGMDFAIRFGEGRWPATHNLKLFDAPLTVLCPPEVAARL
CCCCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCEEEECCEEEECCHHHHHHC
HSPQDLQHERLMRSYRKDEWERWFTAAQVTPWRINGPVFDSSRLMVEGAIHSGGVALAPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEECCCCEEEEECCCCCCEEHHHH
RMFARELREGLLQRPFSAEADLGGYWLTHLKSRDMTPAMKVFVSWIQQQVTDEQQ
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8064842 [H]