Click here to switch to the map view.

The map label for this gene is pfkA [H]

Identifier: 157370498

GI number: 157370498

Start: 2458662

End: 2459642

Strand: Reverse

Name: pfkA [H]

Synonym: Spro_2258

Alternate gene names: 157370498

Gene position: 2459642-2458662 (Counterclockwise)

Preceding gene: 157370499

Following gene: 157370497

Centisome position: 45.14

GC content: 46.69

Gene sequence:

>981_bases
ATGAAAATCGGTGTGGTAATAAGCGGCGGCGACGTCACCGGTATCAATAACTTTATATTCCAGGTCGCGAGACTGACGCA
GGCAGATATCATTCTGTTTAACGGCGGAATACCCGGATTATTGGCCAATCATCATCAAGCTATCAGTCATCGTGACCTGG
TTGATTACGCTATTTCACCAATGCCAATAATGTCTTCCGGCCGCACGGCCAAAAAACTGGTCGCCAGTGAATATGAAAGC
ATCAGCAAACAGCTTAAGGCTTTGCACATTGATGTGCTTATTATGGCCGGTGGCGACGGTTCGCTGCAGTTTTTAAATAC
CCTCAGTGAATTTGGCGTTAACTGTTTTGGCGTAGGCATGACCATCGACAATGATGTTTTTGGCAGCCTTTACAGCATTG
GTTTTTCCACCGCCTGCGAACAGGTGATCAAAGAGGTTTCCAAACTGCGTAACACCGGACGCGCCCTGCCCGGACGGGTG
TTTATGATTGAACTGCTTGGCGGTTACTGCGGGGAACTGACGCTGCAGGCGGCGATCAAATCCAATGCAGACTTTGCCCT
GATCCCGGAATGCCAACATGCCTTAAGCCAACTGGCAGATAAAATAAGCCAGCGACTGGAACAACAAAATAGCGTTATTA
TTTTATGTTCAGAAGGCTATACCAAAGAATATTCACCGGGTTTTCAGGGTGCCATCGACACCATGATTAAACAGCTTGAG
CCAAAAATTGGTGTGCGCATCCGTAAAACCATTATGGGGTATGGCCTGCGCAATGGTGAGCCTACCTGCGAAGAGATTTA
TCAGGGCACTATTATGGCCAGCGAAGTGGTTCGCTGTATTCACTCCGGCATGAAAAACAAGGCGGTCATTATTAATTCCG
CTAACCAACCCATCCCTATTGATTTAATCAGCATGCAAAAACGTTTGGTTGATACCGAAGGACACCATTATAAACTCGCC
AGGCAATTAAATATAATCTGA

Upstream 100 bases:

>100_bases
ATTCACGAAAATATTTGTGATTGTTTTGTGATATCGCCTGCAAATTTTAGCGATTCCAAAAATGACAATGAGCTCCATGG
AACAGATTGGGAGCAGGCAT

Downstream 100 bases:

>100_bases
GGAGAGACTCATGCTTAAAATACTGTGCGTATGTGGCTGTGGCCTCGGCTCCAGCTTTGCCATCGAAATGAGCGCAAGAG
CGGTGCTGAAAAAATTAGAG

Product: phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKIGVVISGGDVTGINNFIFQVARLTQADIILFNGGIPGLLANHHQAISHRDLVDYAISPMPIMSSGRTAKKLVASEYES
ISKQLKALHIDVLIMAGGDGSLQFLNTLSEFGVNCFGVGMTIDNDVFGSLYSIGFSTACEQVIKEVSKLRNTGRALPGRV
FMIELLGGYCGELTLQAAIKSNADFALIPECQHALSQLADKISQRLEQQNSVIILCSEGYTKEYSPGFQGAIDTMIKQLE
PKIGVRIRKTIMGYGLRNGEPTCEEIYQGTIMASEVVRCIHSGMKNKAVIINSANQPIPIDLISMQKRLVDTEGHHYKLA
RQLNII

Sequences:

>Translated_326_residues
MKIGVVISGGDVTGINNFIFQVARLTQADIILFNGGIPGLLANHHQAISHRDLVDYAISPMPIMSSGRTAKKLVASEYES
ISKQLKALHIDVLIMAGGDGSLQFLNTLSEFGVNCFGVGMTIDNDVFGSLYSIGFSTACEQVIKEVSKLRNTGRALPGRV
FMIELLGGYCGELTLQAAIKSNADFALIPECQHALSQLADKISQRLEQQNSVIILCSEGYTKEYSPGFQGAIDTMIKQLE
PKIGVRIRKTIMGYGLRNGEPTCEEIYQGTIMASEVVRCIHSGMKNKAVIINSANQPIPIDLISMQKRLVDTEGHHYKLA
RQLNII
>Mature_326_residues
MKIGVVISGGDVTGINNFIFQVARLTQADIILFNGGIPGLLANHHQAISHRDLVDYAISPMPIMSSGRTAKKLVASEYES
ISKQLKALHIDVLIMAGGDGSLQFLNTLSEFGVNCFGVGMTIDNDVFGSLYSIGFSTACEQVIKEVSKLRNTGRALPGRV
FMIELLGGYCGELTLQAAIKSNADFALIPECQHALSQLADKISQRLEQQNSVIILCSEGYTKEYSPGFQGAIDTMIKQLE
PKIGVRIRKTIMGYGLRNGEPTCEEIYQGTIMASEVVRCIHSGMKNKAVIINSANQPIPIDLISMQKRLVDTEGHHYKLA
RQLNII

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=341, Percent_Identity=23.7536656891496, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI266453748, Length=341, Percent_Identity=23.7536656891496, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4505749, Length=341, Percent_Identity=23.7536656891496, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI266453619, Length=341, Percent_Identity=23.7536656891496, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI48762920, Length=340, Percent_Identity=24.7058823529412, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI11321601, Length=305, Percent_Identity=24.9180327868852, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1790350, Length=330, Percent_Identity=27.5757575757576, Blast_Score=117, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI25147584, Length=314, Percent_Identity=27.0700636942675, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558788, Length=335, Percent_Identity=26.5671641791045, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=22.4137931034483, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24652337, Length=330, Percent_Identity=24.2424242424242, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573326, Length=330, Percent_Identity=24.2424242424242, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17647809, Length=330, Percent_Identity=24.2424242424242, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35561; Mature: 35561

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVVISGGDVTGINNFIFQVARLTQADIILFNGGIPGLLANHHQAISHRDLVDYAISP
CEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
MPIMSSGRTAKKLVASEYESISKQLKALHIDVLIMAGGDGSLQFLNTLSEFGVNCFGVGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHCCCEEEEECE
TIDNDVFGSLYSIGFSTACEQVIKEVSKLRNTGRALPGRVFMIELLGGYCGELTLQAAIK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
SNADFALIPECQHALSQLADKISQRLEQQNSVIILCSEGYTKEYSPGFQGAIDTMIKQLE
CCCCEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHC
PKIGVRIRKTIMGYGLRNGEPTCEEIYQGTIMASEVVRCIHSGMKNKAVIINSANQPIPI
CHHCHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCH
DLISMQKRLVDTEGHHYKLARQLNII
HHHHHHHHHHCCCCCCEEHHHHHCCC
>Mature Secondary Structure
MKIGVVISGGDVTGINNFIFQVARLTQADIILFNGGIPGLLANHHQAISHRDLVDYAISP
CEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
MPIMSSGRTAKKLVASEYESISKQLKALHIDVLIMAGGDGSLQFLNTLSEFGVNCFGVGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHCCCEEEEECE
TIDNDVFGSLYSIGFSTACEQVIKEVSKLRNTGRALPGRVFMIELLGGYCGELTLQAAIK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
SNADFALIPECQHALSQLADKISQRLEQQNSVIILCSEGYTKEYSPGFQGAIDTMIKQLE
CCCCEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHC
PKIGVRIRKTIMGYGLRNGEPTCEEIYQGTIMASEVVRCIHSGMKNKAVIINSANQPIPI
CHHCHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCH
DLISMQKRLVDTEGHHYKLARQLNII
HHHHHHHHHHCCCCCCEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA