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The map label for this gene is rob [H]

Identifier: 157370478

GI number: 157370478

Start: 2438138

End: 2439031

Strand: Direct

Name: rob [H]

Synonym: Spro_2238

Alternate gene names: 157370478

Gene position: 2438138-2439031 (Clockwise)

Preceding gene: 157370472

Following gene: 157370485

Centisome position: 44.75

GC content: 50.11

Gene sequence:

>894_bases
ATGACAAATGAAGATGTATTTTTTATTGAAGAGTTAATTGAGTGGGTTGAGATACATCTGGAGAAACGGCCAAACCTGGA
CGAAGTCGCGCGCATTTCCGGCTATTCCAAATGGCACCTGCAACGCAAGTTCAAACGCATCACCGGCATTCAACTCGCCA
CCTATATCCGTTCACGCATTCTGACGCGCGCTGCGGTGGCGTTACGCATTACCCACCGTTCCATCATCGATATATCAGAC
GAACTGGGCTTCGACTCGCAGCAAACCTTTACCCGTATGTTCAAACAGCGCTTCGAAACCACCCCCAATCGTTACCGCTC
GATGGAACATTGGGACGTGAAGAACCTGATGCCGCGTTTTAGTTTCGACGCCGATTTTGCGGCCGATTTTTACCCTGAGG
TGAAGCGCCTGACGCTGCCGAACATGCAACTGGTGGGGTTCTCGCGGCAGTTGGATTTCTCTTCGGAAACGGAGATCGAA
CATTCGTCCTGTATGGCGATGAAGGACGAGATTTTTACCGACTTCTTCCGTGGTCTGAGTGTGGACTGCCATCGGGTGTA
CAGCATCCATTCCGTCCGCAGCACGGATGACAGCCAATATTCGGCTACCCACATCATGGCGGTGGATCCTGAGAACAAAC
AGGATATTTTGTCGGGCCACCATATCGACACCATCCCTATTGTCGGGCGCGAATATATCTCTATCAACCATCAGGGAAGC
GCCATGGAGTGTACAAAATTTTTCGCTTATCTGATTACCCACGTGATGCCAGGGTTGCGCCATGAGGTGAAGGGGAGCGT
GGAGATGGAGATTATTCAAACTAAGGCGAGGAGTCTGGATTCCAAGATGCGCCAGATTGACGTTGATTATACCTATCTGA
TTTCTATCGATTAA

Upstream 100 bases:

>100_bases
ATGGTTTTTTTTGGTTCGTTTTTTCGCACAAAATAACAACTTTTACATAATCTATGTGGGTTATTATCCCGAGTATTATT
GAGTTAGTGAATGTAAATTA

Downstream 100 bases:

>100_bases
AAAGTGCCTTGCGCCCTGCAAGGCACCTCTCACCGTCACTCTTTATCCGTATCCGCCGTGGTCAGACTTTGCGCCGCTCT
TTTAGCTTTGGCGCGGGCAA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTNEDVFFIEELIEWVEIHLEKRPNLDEVARISGYSKWHLQRKFKRITGIQLATYIRSRILTRAAVALRITHRSIIDISD
ELGFDSQQTFTRMFKQRFETTPNRYRSMEHWDVKNLMPRFSFDADFAADFYPEVKRLTLPNMQLVGFSRQLDFSSETEIE
HSSCMAMKDEIFTDFFRGLSVDCHRVYSIHSVRSTDDSQYSATHIMAVDPENKQDILSGHHIDTIPIVGREYISINHQGS
AMECTKFFAYLITHVMPGLRHEVKGSVEMEIIQTKARSLDSKMRQIDVDYTYLISID

Sequences:

>Translated_297_residues
MTNEDVFFIEELIEWVEIHLEKRPNLDEVARISGYSKWHLQRKFKRITGIQLATYIRSRILTRAAVALRITHRSIIDISD
ELGFDSQQTFTRMFKQRFETTPNRYRSMEHWDVKNLMPRFSFDADFAADFYPEVKRLTLPNMQLVGFSRQLDFSSETEIE
HSSCMAMKDEIFTDFFRGLSVDCHRVYSIHSVRSTDDSQYSATHIMAVDPENKQDILSGHHIDTIPIVGREYISINHQGS
AMECTKFFAYLITHVMPGLRHEVKGSVEMEIIQTKARSLDSKMRQIDVDYTYLISID
>Mature_296_residues
TNEDVFFIEELIEWVEIHLEKRPNLDEVARISGYSKWHLQRKFKRITGIQLATYIRSRILTRAAVALRITHRSIIDISDE
LGFDSQQTFTRMFKQRFETTPNRYRSMEHWDVKNLMPRFSFDADFAADFYPEVKRLTLPNMQLVGFSRQLDFSSETEIEH
SSCMAMKDEIFTDFFRGLSVDCHRVYSIHSVRSTDDSQYSATHIMAVDPENKQDILSGHHIDTIPIVGREYISINHQGSA
MECTKFFAYLITHVMPGLRHEVKGSVEMEIIQTKARSLDSKMRQIDVDYTYLISID

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=257, Percent_Identity=33.852140077821, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1790497, Length=98, Percent_Identity=51.0204081632653, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081928, Length=104, Percent_Identity=45.1923076923077, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 34795; Mature: 34664

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEDVFFIEELIEWVEIHLEKRPNLDEVARISGYSKWHLQRKFKRITGIQLATYIRSRI
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTRAAVALRITHRSIIDISDELGFDSQQTFTRMFKQRFETTPNRYRSMEHWDVKNLMPRF
HHHHHHHHHHHHHHHEECHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHCCCC
SFDADFAADFYPEVKRLTLPNMQLVGFSRQLDFSSETEIEHSSCMAMKDEIFTDFFRGLS
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
VDCHRVYSIHSVRSTDDSQYSATHIMAVDPENKQDILSGHHIDTIPIVGREYISINHQGS
CCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCEEEECCCEEEEECCCCC
AMECTKFFAYLITHVMPGLRHEVKGSVEMEIIQTKARSLDSKMRQIDVDYTYLISID
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure 
TNEDVFFIEELIEWVEIHLEKRPNLDEVARISGYSKWHLQRKFKRITGIQLATYIRSRI
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTRAAVALRITHRSIIDISDELGFDSQQTFTRMFKQRFETTPNRYRSMEHWDVKNLMPRF
HHHHHHHHHHHHHHHEECHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHCCCC
SFDADFAADFYPEVKRLTLPNMQLVGFSRQLDFSSETEIEHSSCMAMKDEIFTDFFRGLS
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
VDCHRVYSIHSVRSTDDSQYSATHIMAVDPENKQDILSGHHIDTIPIVGREYISINHQGS
CCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCEEEECCCEEEEECCCCC
AMECTKFFAYLITHVMPGLRHEVKGSVEMEIIQTKARSLDSKMRQIDVDYTYLISID
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]