Click here to switch to the map view.

The map label for this gene is ptrB [C]

Identifier: 157370472

GI number: 157370472

Start: 2430414

End: 2432504

Strand: Direct

Name: ptrB [C]

Synonym: Spro_2232

Alternate gene names: 157370472

Gene position: 2430414-2432504 (Clockwise)

Preceding gene: 157370471

Following gene: 157370478

Centisome position: 44.6

GC content: 56.86

Gene sequence:

>2091_bases
ATGACTTCATTACCGAGCAGTATCCGCCTGGCGATGGCGGCAGAAAATGGCGCCAGCTCCACCGATGAATTTTTATGGCT
GGAACAGCTGCAGGGCAAGGCCGTACAGGCGTGGATTAAACAGGAAAACCAGCGTACCACCGCGCGTTTCGCCCAGGGTG
AAGGCTTCCAACGGGTGGAAAAACAGGTGCTGGATATTCTCAATAAAGAGAGCCAAATTCCGTGGGTCGCCAAGCGCGGC
GATTACTATTACAACTTCTGGCAGGATCAAACCAATCCGCGTGGCTTATGGCGGCGCACTACACTGCAGGAGTATCGCAA
GGCCAAGCCAGCCTGGGAAACGGTGCTGGATATCGATGCGTTAGGCAAGGCCGAGGGCAAAGATTGGGTATTCCACGGCT
CGCAGTCGTTAGCGCCGGAGTATCGCTATTGCCTGCTGGAACTGTCGCCGGACGGCGGTGACGCTACCGAAATCCGTGAG
TTTGATCTGGTCAACAAAGAGTTTGTCAAAGACGGCTTTGTGCTGCCGGTAGCGAAAAGCCAGGCCTCGTGGATTGATAA
AGACACGCTGTTTATCGCCACTGATTTCGGCGCAGGCTCAATGACTCAGTCCGGCTATGCGCGTATTGCCAAACGCTGGC
GGCGGGGTACGCCACTGAGCGCCGCCCAGACCTTGTATGAAGCGCAACCGCAGGATATGACGGTGTTCGCTTATCATGAC
CAGACGCCGGGGTTTGAACGCAACTACGTGGGCCGCAGTCTGGATTTCTACCGCCGTGAAACCTATCTGCTGACCGCGCA
AGATCAACAGGTCAAAATTGATATCCCCGCCGATGCCGAATTCAGCACGCACCGCGAGTGGATATTGATTAAACCGAGCA
GCGACTGGGAAGTGGGCGGAGCGCGCTATCCTTCGGGTTCGCTGTTGGCGGCCCATTTTGATGATTATCTGGCGGGCAAA
CGTGAGCTAACGGTGTTATTTGCCCCGACCGCTCAGGCGGCGCTGAGCGGTTACAGTGGTACTCGCGACCACCTGATCCT
CAGCATCATGGACAACGTGGTCAATCGATTGGAAGTCCTGACACCGCAGGGCAAAACCTGGCAGCGCAAGCCGCTGGGTA
AGCCAGGGGCGATCAGCACTATTTCCGCCGGTGGGGTCGATGACGAAACCAACGACTATTTCCTGACGATCAGCGGATTC
CTGCAACCGACTTCGCTGTATCTGGGCAATCTCGATGGCGGTGAGGCGGAACTGCTCAAGCAGGGGCCACAGGACTTTGA
TGCCAGCGGTTATCAGGTCAGCCAGCATTTCACCGAGTCTAAAGACGGCACGCGCGTACCGTATTTCCAAATTTCAGCCA
AGGGCATCAAGCTTGACGGCAGTAACCCAACCTTGCTGTATGGCTATGGCGGGTTCGAAGTGTCGCTCTTGCCGGGCTAC
ATGGGAGCTAAGGCCCCGGCCTGGCTGGAACGCGGTGGGGTGTACGTGGTGGCGAATATTCGTGGCGGTGGCGAGTATGG
CCCGGCCTGGCATCAGGCGGCGTTAAAGCAAAACCGGCATCGCGCCTATGAGGACTTTGCCGCCGTGGCTGAAGATTTGA
TCGCACGAAAAGTGACATCGTCACAACATCTGGGCGCACGGGGTGGCAGTAACGGTGGCTTGCTGGTGGGCAATATGCTG
ACGCTGTACCCGCAACTTTTCGGCTGCATCGTCTGTGAAGTGCCGTTGCTGGATATGCAGCGCTATACCCAGCTTTCCGC
CGGTGCCTCCTGGATTGCCGAATATGGCGACCCGAGCAAGGCGGAAGAATGGGCCTACATCAAAACCTTCTCGCCGTATC
ACAATATCAAAGCCGGAGTGTCCTACCCGCCGGTGCTGTTTTACACCGCCACCAGTGACGATCGGGTCAACCCGTCGCAT
GCCCGTAAAATGGCGGCACGCATGCAGCAGATGGGGTATCCGCAGGCCTATTTCTATGAGAATACCGAAGGCGGGCACAG
TGCCGCTGCGGACAAGAAACAGGCCGCATTCCACGGTGCACTGGTCAGTGAGTTTATGTGGGACAATTTGAGCGCAAAAT
CCACAGCCTGA

Upstream 100 bases:

>100_bases
ATTATTAACCATCAAAGGGAGCTTGAGTTTGCCTATTACCCACCGCAACCGACGTCAGTTTCTTAAACTCGGCGCACAAA
CCGCAGGAGCCGTATTCGCG

Downstream 100 bases:

>100_bases
TGACATAAAAAAGCCGGGGCAAATTAATGCCCCGGCTTTCTTTTACCTGTCTGAATCCGTTGTCAGCAATCAGTTGGTGA
TAGCGGCCGCCTGAGCTTTC

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MTSLPSSIRLAMAAENGASSTDEFLWLEQLQGKAVQAWIKQENQRTTARFAQGEGFQRVEKQVLDILNKESQIPWVAKRG
DYYYNFWQDQTNPRGLWRRTTLQEYRKAKPAWETVLDIDALGKAEGKDWVFHGSQSLAPEYRYCLLELSPDGGDATEIRE
FDLVNKEFVKDGFVLPVAKSQASWIDKDTLFIATDFGAGSMTQSGYARIAKRWRRGTPLSAAQTLYEAQPQDMTVFAYHD
QTPGFERNYVGRSLDFYRRETYLLTAQDQQVKIDIPADAEFSTHREWILIKPSSDWEVGGARYPSGSLLAAHFDDYLAGK
RELTVLFAPTAQAALSGYSGTRDHLILSIMDNVVNRLEVLTPQGKTWQRKPLGKPGAISTISAGGVDDETNDYFLTISGF
LQPTSLYLGNLDGGEAELLKQGPQDFDASGYQVSQHFTESKDGTRVPYFQISAKGIKLDGSNPTLLYGYGGFEVSLLPGY
MGAKAPAWLERGGVYVVANIRGGGEYGPAWHQAALKQNRHRAYEDFAAVAEDLIARKVTSSQHLGARGGSNGGLLVGNML
TLYPQLFGCIVCEVPLLDMQRYTQLSAGASWIAEYGDPSKAEEWAYIKTFSPYHNIKAGVSYPPVLFYTATSDDRVNPSH
ARKMAARMQQMGYPQAYFYENTEGGHSAAADKKQAAFHGALVSEFMWDNLSAKSTA

Sequences:

>Translated_696_residues
MTSLPSSIRLAMAAENGASSTDEFLWLEQLQGKAVQAWIKQENQRTTARFAQGEGFQRVEKQVLDILNKESQIPWVAKRG
DYYYNFWQDQTNPRGLWRRTTLQEYRKAKPAWETVLDIDALGKAEGKDWVFHGSQSLAPEYRYCLLELSPDGGDATEIRE
FDLVNKEFVKDGFVLPVAKSQASWIDKDTLFIATDFGAGSMTQSGYARIAKRWRRGTPLSAAQTLYEAQPQDMTVFAYHD
QTPGFERNYVGRSLDFYRRETYLLTAQDQQVKIDIPADAEFSTHREWILIKPSSDWEVGGARYPSGSLLAAHFDDYLAGK
RELTVLFAPTAQAALSGYSGTRDHLILSIMDNVVNRLEVLTPQGKTWQRKPLGKPGAISTISAGGVDDETNDYFLTISGF
LQPTSLYLGNLDGGEAELLKQGPQDFDASGYQVSQHFTESKDGTRVPYFQISAKGIKLDGSNPTLLYGYGGFEVSLLPGY
MGAKAPAWLERGGVYVVANIRGGGEYGPAWHQAALKQNRHRAYEDFAAVAEDLIARKVTSSQHLGARGGSNGGLLVGNML
TLYPQLFGCIVCEVPLLDMQRYTQLSAGASWIAEYGDPSKAEEWAYIKTFSPYHNIKAGVSYPPVLFYTATSDDRVNPSH
ARKMAARMQQMGYPQAYFYENTEGGHSAAADKKQAAFHGALVSEFMWDNLSAKSTA
>Mature_695_residues
TSLPSSIRLAMAAENGASSTDEFLWLEQLQGKAVQAWIKQENQRTTARFAQGEGFQRVEKQVLDILNKESQIPWVAKRGD
YYYNFWQDQTNPRGLWRRTTLQEYRKAKPAWETVLDIDALGKAEGKDWVFHGSQSLAPEYRYCLLELSPDGGDATEIREF
DLVNKEFVKDGFVLPVAKSQASWIDKDTLFIATDFGAGSMTQSGYARIAKRWRRGTPLSAAQTLYEAQPQDMTVFAYHDQ
TPGFERNYVGRSLDFYRRETYLLTAQDQQVKIDIPADAEFSTHREWILIKPSSDWEVGGARYPSGSLLAAHFDDYLAGKR
ELTVLFAPTAQAALSGYSGTRDHLILSIMDNVVNRLEVLTPQGKTWQRKPLGKPGAISTISAGGVDDETNDYFLTISGFL
QPTSLYLGNLDGGEAELLKQGPQDFDASGYQVSQHFTESKDGTRVPYFQISAKGIKLDGSNPTLLYGYGGFEVSLLPGYM
GAKAPAWLERGGVYVVANIRGGGEYGPAWHQAALKQNRHRAYEDFAAVAEDLIARKVTSSQHLGARGGSNGGLLVGNMLT
LYPQLFGCIVCEVPLLDMQRYTQLSAGASWIAEYGDPSKAEEWAYIKTFSPYHNIKAGVSYPPVLFYTATSDDRVNPSHA
RKMAARMQQMGYPQAYFYENTEGGHSAAADKKQAAFHGALVSEFMWDNLSAKSTA

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=316, Percent_Identity=38.2911392405063, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI284172438, Length=202, Percent_Identity=26.2376237623762, Blast_Score=66, Evalue=1e-10,
Organism=Homo sapiens, GI284172431, Length=202, Percent_Identity=26.2376237623762, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1788150, Length=671, Percent_Identity=22.8017883755589, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24583414, Length=318, Percent_Identity=36.1635220125786, Blast_Score=195, Evalue=1e-49,
Organism=Drosophila melanogaster, GI221510989, Length=285, Percent_Identity=34.3859649122807, Blast_Score=167, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 77355; Mature: 77224

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLPSSIRLAMAAENGASSTDEFLWLEQLQGKAVQAWIKQENQRTTARFAQGEGFQRVE
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCHHHHH
KQVLDILNKESQIPWVAKRGDYYYNFWQDQTNPRGLWRRTTLQEYRKAKPAWETVLDIDA
HHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
LGKAEGKDWVFHGSQSLAPEYRYCLLELSPDGGDATEIREFDLVNKEFVKDGFVLPVAKS
CCCCCCCCEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
QASWIDKDTLFIATDFGAGSMTQSGYARIAKRWRRGTPLSAAQTLYEAQPQDMTVFAYHD
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEC
QTPGFERNYVGRSLDFYRRETYLLTAQDQQVKIDIPADAEFSTHREWILIKPSSDWEVGG
CCCCCCCCCCCCCHHHHCCEEEEEEECCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCC
ARYPSGSLLAAHFDDYLAGKRELTVLFAPTAQAALSGYSGTRDHLILSIMDNVVNRLEVL
CCCCCCCEEEEEHHHHHCCCEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHEEE
TPQGKTWQRKPLGKPGAISTISAGGVDDETNDYFLTISGFLQPTSLYLGNLDGGEAELLK
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHH
QGPQDFDASGYQVSQHFTESKDGTRVPYFQISAKGIKLDGSNPTLLYGYGGFEVSLLPGY
CCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECEEEECCCCCEEEEEECCEEEEEECCC
MGAKAPAWLERGGVYVVANIRGGGEYGPAWHQAALKQNRHRAYEDFAAVAEDLIARKVTS
CCCCCCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SQHLGARGGSNGGLLVGNMLTLYPQLFGCIVCEVPLLDMQRYTQLSAGASWIAEYGDPSK
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCC
AEEWAYIKTFSPYHNIKAGVSYPPVLFYTATSDDRVNPSHARKMAARMQQMGYPQAYFYE
CCCEEEEEECCCHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
NTEGGHSAAADKKQAAFHGALVSEFMWDNLSAKSTA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSLPSSIRLAMAAENGASSTDEFLWLEQLQGKAVQAWIKQENQRTTARFAQGEGFQRVE
CCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCHHHHH
KQVLDILNKESQIPWVAKRGDYYYNFWQDQTNPRGLWRRTTLQEYRKAKPAWETVLDIDA
HHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
LGKAEGKDWVFHGSQSLAPEYRYCLLELSPDGGDATEIREFDLVNKEFVKDGFVLPVAKS
CCCCCCCCEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
QASWIDKDTLFIATDFGAGSMTQSGYARIAKRWRRGTPLSAAQTLYEAQPQDMTVFAYHD
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEC
QTPGFERNYVGRSLDFYRRETYLLTAQDQQVKIDIPADAEFSTHREWILIKPSSDWEVGG
CCCCCCCCCCCCCHHHHCCEEEEEEECCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCC
ARYPSGSLLAAHFDDYLAGKRELTVLFAPTAQAALSGYSGTRDHLILSIMDNVVNRLEVL
CCCCCCCEEEEEHHHHHCCCEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHEEE
TPQGKTWQRKPLGKPGAISTISAGGVDDETNDYFLTISGFLQPTSLYLGNLDGGEAELLK
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHH
QGPQDFDASGYQVSQHFTESKDGTRVPYFQISAKGIKLDGSNPTLLYGYGGFEVSLLPGY
CCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECEEEECCCCCEEEEEECCEEEEEECCC
MGAKAPAWLERGGVYVVANIRGGGEYGPAWHQAALKQNRHRAYEDFAAVAEDLIARKVTS
CCCCCCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SQHLGARGGSNGGLLVGNMLTLYPQLFGCIVCEVPLLDMQRYTQLSAGASWIAEYGDPSK
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCC
AEEWAYIKTFSPYHNIKAGVSYPPVLFYTATSDDRVNPSHARKMAARMQQMGYPQAYFYE
CCCEEEEEECCCHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
NTEGGHSAAADKKQAAFHGALVSEFMWDNLSAKSTA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]