| Definition | Serratia proteamaculans 568 chromosome, complete genome. |
|---|---|
| Accession | NC_009832 |
| Length | 5,448,853 |
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The map label for this gene is pckA [H]
Identifier: 157370340
GI number: 157370340
Start: 2293423
End: 2295042
Strand: Reverse
Name: pckA [H]
Synonym: Spro_2098
Alternate gene names: 157370340
Gene position: 2295042-2293423 (Counterclockwise)
Preceding gene: 157370343
Following gene: 157370339
Centisome position: 42.12
GC content: 56.98
Gene sequence:
>1620_bases ATGTCTTCAATCGCGATTACCCCACAAACACTGGCCCGTTACGGCATCCATCAGGCTACCGAAATCGTCCATAACCCAAG CTACGAGCAACTGTTTACCGAGGAAACCCGTGCCGACCTGCCGGCACTGGAACGCGGTACGGTAACAACGCTGGGCGCGG TCAACGTCGATACTGGTGAATTCACCGGCCGTTCACCGAAAGATAAATACATCGTGCGTGACGACACCACGCGCGATACC GTCTGGTGGTCCGATAACGGCACCGGCAAAAACGACAACCAACCGCTATCGCCAGAAGTCTGGCAGCATCTGAAAACGCT GGTGGGTAACCAACTTTCCGGCAAGCGTCTGTTTGTGGTGGACGCCTTCTGCGGCGCCAATGCCGACACCCGCCTGAAGG TGCGTTTCATCACCGAGGTGGCCTGGCAGGCGCACTTTGTCAAAAACATGTTTATCCGCCCGACGGATGAAGAACTGCAG AATTTCGAACCGGACTTCGTGGTAATGAACGGGGCCAAATGCACCAACCCGGACTGGCAACAGCAGGGGCTGCATTCAGA GAACTTCGTTGCCTTTAACCTGACCGAGCGTATCCAGCTGATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAGGGCC TGTTCTCAATCATGAACTACCTGCTGCCGTTGCAGGGTATCGCCTCGATGCACTGTTCGGCCAACGTCGGCGAACAGGGT GACGTGGCAGTGTTCTTTGGCCTGTCCGGTACCGGTAAAACCACCCTCTCCACCGATCCCAAGCGTCAGTTGATCGGCGA TGACGAGCACGGCTGGGATCATGACGGCGTGTTCAACTTTGAAGGCGGCTGCTACGCCAAGACCATCAAACTGTCGCCGC AGGCCGAACCTGAGATCTATCAGGCTATCCGTCGCAACGCACTGTTGGAAAACGTACAGGTGCTGGCCGATGGCAGCATT GATTTTGATGACGCCGGCAAGACTGAAAACACCCGCGTTTCCTACCCGATTGACCACATTGAAAACATCGTCAAGCCAGT GTCCAAGGCCGGTCATGCGAAGAAAATCATCTTCCTGACCGCCGATGCTTTCGGGGTATTGCCGCCGGTATCACGCCTGA CGCCGGAACAGACTCAGTACCACTTCCTGTCTGGTTTTACCGCCAAGCTGGCCGGTACCGAGCGCGGCATCACCACGCCA ACCCCAACCTTCTCCGCCTGTTTCGGCGCCGCGTTCCTGACGCTGCACCCGACGCAGTACGCCGAAGTGCTGGTGAAACG TATGGAAGCGGCCGGAGCGCAGGCCTATCTGGTGAATACCGGTTGGAACGGCAGCGGCAAGCGTATTTCCATTAAGGACA CGCGCGGCATCATCGACGCCATTTTGAACGGGGAGATTGAACACGCCGAAACCTTCACTCTGCCGATCTTTGGCCTGGAA GTCCCGACCGCGCTGCCGGGAGTTGACCCTTCCATTCTCGACCCGCGTAAAACTTACGCGGACGAATCGCTTTGGCAAGA GAAAGCTCAGGATCTGGCGCAGCGCTTTATCGATAACTTTGCCAAATACACCGTGACACCGGCGGGTGAAAAACTGGTCA GCGCCGGCCCTAAACTCTAA
Upstream 100 bases:
>100_bases AGAAAACAGGCAATAATATATGCCCCATGGCTTTCAGATTGTCGCCGACAGCGCGGCAGCCCGAAAGTCTGCAGGCATAG AACTAATAAGGAAGTAGGAT
Downstream 100 bases:
>100_bases CTAGCCTGCAGGACGCGCCAATGCCCTGCAGACCATCAAACTGAATTTACCGCATTATTGATCTATACCTCCATTCTCAG GGTGCCCCATGGCACCCTAT
Product: phosphoenolpyruvate carboxykinase
Products: NA
Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]
Number of amino acids: Translated: 539; Mature: 538
Protein sequence:
>539_residues MSSIAITPQTLARYGIHQATEIVHNPSYEQLFTEETRADLPALERGTVTTLGAVNVDTGEFTGRSPKDKYIVRDDTTRDT VWWSDNGTGKNDNQPLSPEVWQHLKTLVGNQLSGKRLFVVDAFCGANADTRLKVRFITEVAWQAHFVKNMFIRPTDEELQ NFEPDFVVMNGAKCTNPDWQQQGLHSENFVAFNLTERIQLIGGTWYGGEMKKGLFSIMNYLLPLQGIASMHCSANVGEQG DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDHDGVFNFEGGCYAKTIKLSPQAEPEIYQAIRRNALLENVQVLADGSI DFDDAGKTENTRVSYPIDHIENIVKPVSKAGHAKKIIFLTADAFGVLPPVSRLTPEQTQYHFLSGFTAKLAGTERGITTP TPTFSACFGAAFLTLHPTQYAEVLVKRMEAAGAQAYLVNTGWNGSGKRISIKDTRGIIDAILNGEIEHAETFTLPIFGLE VPTALPGVDPSILDPRKTYADESLWQEKAQDLAQRFIDNFAKYTVTPAGEKLVSAGPKL
Sequences:
>Translated_539_residues MSSIAITPQTLARYGIHQATEIVHNPSYEQLFTEETRADLPALERGTVTTLGAVNVDTGEFTGRSPKDKYIVRDDTTRDT VWWSDNGTGKNDNQPLSPEVWQHLKTLVGNQLSGKRLFVVDAFCGANADTRLKVRFITEVAWQAHFVKNMFIRPTDEELQ NFEPDFVVMNGAKCTNPDWQQQGLHSENFVAFNLTERIQLIGGTWYGGEMKKGLFSIMNYLLPLQGIASMHCSANVGEQG DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDHDGVFNFEGGCYAKTIKLSPQAEPEIYQAIRRNALLENVQVLADGSI DFDDAGKTENTRVSYPIDHIENIVKPVSKAGHAKKIIFLTADAFGVLPPVSRLTPEQTQYHFLSGFTAKLAGTERGITTP TPTFSACFGAAFLTLHPTQYAEVLVKRMEAAGAQAYLVNTGWNGSGKRISIKDTRGIIDAILNGEIEHAETFTLPIFGLE VPTALPGVDPSILDPRKTYADESLWQEKAQDLAQRFIDNFAKYTVTPAGEKLVSAGPKL >Mature_538_residues SSIAITPQTLARYGIHQATEIVHNPSYEQLFTEETRADLPALERGTVTTLGAVNVDTGEFTGRSPKDKYIVRDDTTRDTV WWSDNGTGKNDNQPLSPEVWQHLKTLVGNQLSGKRLFVVDAFCGANADTRLKVRFITEVAWQAHFVKNMFIRPTDEELQN FEPDFVVMNGAKCTNPDWQQQGLHSENFVAFNLTERIQLIGGTWYGGEMKKGLFSIMNYLLPLQGIASMHCSANVGEQGD VAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDHDGVFNFEGGCYAKTIKLSPQAEPEIYQAIRRNALLENVQVLADGSID FDDAGKTENTRVSYPIDHIENIVKPVSKAGHAKKIIFLTADAFGVLPPVSRLTPEQTQYHFLSGFTAKLAGTERGITTPT PTFSACFGAAFLTLHPTQYAEVLVKRMEAAGAQAYLVNTGWNGSGKRISIKDTRGIIDAILNGEIEHAETFTLPIFGLEV PTALPGVDPSILDPRKTYADESLWQEKAQDLAQRFIDNFAKYTVTPAGEKLVSAGPKL
Specific function: Rate-limiting gluconeogenic enzyme. [C]
COG id: COG1866
COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]
Homologues:
Organism=Escherichia coli, GI1789807, Length=535, Percent_Identity=81.3084112149533, Blast_Score=915, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6322950, Length=507, Percent_Identity=46.5483234714004, Blast_Score=444, Evalue=1e-125,
Paralogues:
None
Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001272 - InterPro: IPR013035 - InterPro: IPR008210 - InterPro: IPR015994 [H]
Pfam domain/function: PF01293 PEPCK_ATP [H]
EC number: =4.1.1.49 [H]
Molecular weight: Translated: 59393; Mature: 59261
Theoretical pI: Translated: 5.35; Mature: 5.35
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.3 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 2.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSSIAITPQTLARYGIHQATEIVHNPSYEQLFTEETRADLPALERGTVTTLGAVNVDTGE CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCC FTGRSPKDKYIVRDDTTRDTVWWSDNGTGKNDNQPLSPEVWQHLKTLVGNQLSGKRLFVV CCCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEE DAFCGANADTRLKVRFITEVAWQAHFVKNMFIRPTDEELQNFEPDFVVMNGAKCTNPDWQ EECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCCHH QQGLHSENFVAFNLTERIQLIGGTWYGGEMKKGLFSIMNYLLPLQGIASMHCSANVGEQG HCCCCCCCEEEEEHHHEEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDHDGVFNFEGGCYAKTIKLSPQAEPEIY CEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCHHHH QAIRRNALLENVQVLADGSIDFDDAGKTENTRVSYPIDHIENIVKPVSKAGHAKKIIFLT HHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEE ADAFGVLPPVSRLTPEQTQYHFLSGFTAKLAGTERGITTPTPTFSACFGAAFLTLHPTQY CCCCCCCCCHHHCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHEEEECCHHH AEVLVKRMEAAGAQAYLVNTGWNGSGKRISIKDTRGIIDAILNGEIEHAETFTLPIFGLE HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCEEEEEEEECCC VPTALPGVDPSILDPRKTYADESLWQEKAQDLAQRFIDNFAKYTVTPAGEKLVSAGPKL CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCC >Mature Secondary Structure SSIAITPQTLARYGIHQATEIVHNPSYEQLFTEETRADLPALERGTVTTLGAVNVDTGE CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCC FTGRSPKDKYIVRDDTTRDTVWWSDNGTGKNDNQPLSPEVWQHLKTLVGNQLSGKRLFVV CCCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEE DAFCGANADTRLKVRFITEVAWQAHFVKNMFIRPTDEELQNFEPDFVVMNGAKCTNPDWQ EECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCCHH QQGLHSENFVAFNLTERIQLIGGTWYGGEMKKGLFSIMNYLLPLQGIASMHCSANVGEQG HCCCCCCCEEEEEHHHEEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDHDGVFNFEGGCYAKTIKLSPQAEPEIY CEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCHHHH QAIRRNALLENVQVLADGSIDFDDAGKTENTRVSYPIDHIENIVKPVSKAGHAKKIIFLT HHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEE ADAFGVLPPVSRLTPEQTQYHFLSGFTAKLAGTERGITTPTPTFSACFGAAFLTLHPTQY CCCCCCCCCHHHCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHEEEECCHHH AEVLVKRMEAAGAQAYLVNTGWNGSGKRISIKDTRGIIDAILNGEIEHAETFTLPIFGLE HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCEEEEEEEECCC VPTALPGVDPSILDPRKTYADESLWQEKAQDLAQRFIDNFAKYTVTPAGEKLVSAGPKL CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 14528314 [H]