Click here to switch to the map view.

The map label for this gene is ligB [H]

Identifier: 157370338

GI number: 157370338

Start: 2290413

End: 2291675

Strand: Reverse

Name: ligB [H]

Synonym: Spro_2096

Alternate gene names: 157370338

Gene position: 2291675-2290413 (Counterclockwise)

Preceding gene: 157370339

Following gene: 157370337

Centisome position: 42.06

GC content: 60.1

Gene sequence:

>1263_bases
ATGGCTAAGATCATTGGCGGATTGGCGGTGTCACACACCCCAACCATCGGTTTTGCAGTGGATCACAACAAGCAGAACGA
AACGGCCTGGGCGCCCATTTTTGACGGTTTCGCCCCGATGCAGCAATGGCTGGAAGAGAAAAAGCCGGACGTGTTGCTGT
ATGTCTTCAACGACCACGTGACCTCGTTTTTCTTCGATCACTATTCGGCATTCGTGCTCGGCATTGACGACAGCTACGCG
GTGGCGGACGAAGGTGGCGGCCCGCGTGATTTGCCGCCGATTCGCGGCCATGCGGCGCTGTCACAGCACATCGGTGCCAG
CCTGATGGCCGACGAGTTTGATATGTCGTTCTTCCAGGATAAGCCGCTCGATCACGGGCTGTTCTCTCCGCTCTCCGCCC
TGCTGCCGTGGCAGAATGGCTGGCCGATGCAGGTGGTGCCGCTGCAGGTCGGGGTGCTGCAGTTCCCGATCCCTTCGGCT
CGCCGCTGCTACAAGCTCGGTCAGGCGCTGCGCCGGGCAATTGAAAGCTTCCCGGAAGACTTGCGCGTCGCGGTGGTGGC
CACCGGCGGCGTCTCGCATCAGGTGCATGGCGAACGTTGCGGTTTTAATAATCCGCAGTGGGATGAGCAGTTTGTCGACC
TGCTGGTCAATGACCCGGAGCGCCTGACCGAAATCACGCTGGCGGAGTACGCCACCTTGGGTGGGCTGGAGGGCGCGGAG
GTGATCATGTGGCTGATTATGCGCGGCGCCCTGTCGGCCAACGTCGAAAAACTGCATCAGGCCTATTACCTGCCGTCCAT
GACCGGTATCGCCACGCTGATCCTGGAAAACCAGGCGCGCGAGGCACCGGTAGATGTCCATCAGCGCCAGCGCGACAAAA
TCAACCTGCAACTGGCGGGGGTTGAGAAACTGCCGGGCACCTATCCCTTTACCCATGCGCGCAGCCTGAAAGCCATCCGC
ATCAACCGTTTCCTGCACAAACTGATCCAGCCGGCCTGGCGCGAACGCTTCAATAACGCCCAGCAGGCGCTGTTCGACGA
AGCGCAGCTCACCACTGAGGAACAGCAGCTGCTGCGCGAGCTGGACTGGCGCGGGCTGATCCATTACGGCGTCAGTTTCT
TCCTGTTGGAAAAGCTCGGGGCAGTGGTCGGGGTATCCAACCTGCATATCTACTCGGCGATGCGTGGCCAGACGCTGGAT
GAGTTCCAGCAAACCCGCAATCAGCAAGTGTTGTATTCCGTTGCGGGGAAAGCGCCAAAATGA

Upstream 100 bases:

>100_bases
ACATCCGGCGGAAATGGTTAAGGGCATCGACATCACCGATCTGCCCGCGTTGTCACGCACCATGAATAACCGGTAATTCT
TATTAATACAGAGGTAGACG

Downstream 100 bases:

>100_bases
ATATTTTTGACCAGCCGAAAATAGACTGTCACCACCATATTTTCGACCCACAGCGCTTTCCCTACGTGGCGGACAGTGGT
TACAACCCGGAAGGCCACGA

Product: protocatechuate 4,5-dioxygenase

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MAKIIGGLAVSHTPTIGFAVDHNKQNETAWAPIFDGFAPMQQWLEEKKPDVLLYVFNDHVTSFFFDHYSAFVLGIDDSYA
VADEGGGPRDLPPIRGHAALSQHIGASLMADEFDMSFFQDKPLDHGLFSPLSALLPWQNGWPMQVVPLQVGVLQFPIPSA
RRCYKLGQALRRAIESFPEDLRVAVVATGGVSHQVHGERCGFNNPQWDEQFVDLLVNDPERLTEITLAEYATLGGLEGAE
VIMWLIMRGALSANVEKLHQAYYLPSMTGIATLILENQAREAPVDVHQRQRDKINLQLAGVEKLPGTYPFTHARSLKAIR
INRFLHKLIQPAWRERFNNAQQALFDEAQLTTEEQQLLRELDWRGLIHYGVSFFLLEKLGAVVGVSNLHIYSAMRGQTLD
EFQQTRNQQVLYSVAGKAPK

Sequences:

>Translated_420_residues
MAKIIGGLAVSHTPTIGFAVDHNKQNETAWAPIFDGFAPMQQWLEEKKPDVLLYVFNDHVTSFFFDHYSAFVLGIDDSYA
VADEGGGPRDLPPIRGHAALSQHIGASLMADEFDMSFFQDKPLDHGLFSPLSALLPWQNGWPMQVVPLQVGVLQFPIPSA
RRCYKLGQALRRAIESFPEDLRVAVVATGGVSHQVHGERCGFNNPQWDEQFVDLLVNDPERLTEITLAEYATLGGLEGAE
VIMWLIMRGALSANVEKLHQAYYLPSMTGIATLILENQAREAPVDVHQRQRDKINLQLAGVEKLPGTYPFTHARSLKAIR
INRFLHKLIQPAWRERFNNAQQALFDEAQLTTEEQQLLRELDWRGLIHYGVSFFLLEKLGAVVGVSNLHIYSAMRGQTLD
EFQQTRNQQVLYSVAGKAPK
>Mature_419_residues
AKIIGGLAVSHTPTIGFAVDHNKQNETAWAPIFDGFAPMQQWLEEKKPDVLLYVFNDHVTSFFFDHYSAFVLGIDDSYAV
ADEGGGPRDLPPIRGHAALSQHIGASLMADEFDMSFFQDKPLDHGLFSPLSALLPWQNGWPMQVVPLQVGVLQFPIPSAR
RCYKLGQALRRAIESFPEDLRVAVVATGGVSHQVHGERCGFNNPQWDEQFVDLLVNDPERLTEITLAEYATLGGLEGAEV
IMWLIMRGALSANVEKLHQAYYLPSMTGIATLILENQAREAPVDVHQRQRDKINLQLAGVEKLPGTYPFTHARSLKAIRI
NRFLHKLIQPAWRERFNNAQQALFDEAQLTTEEQQLLRELDWRGLIHYGVSFFLLEKLGAVVGVSNLHIYSAMRGQTLDE
FQQTRNQQVLYSVAGKAPK

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 47166; Mature: 47035

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIIGGLAVSHTPTIGFAVDHNKQNETAWAPIFDGFAPMQQWLEEKKPDVLLYVFNDHV
CCCCCCCEEECCCCCEEEEECCCCCCCCEECHHHCCHHHHHHHHHHCCCCEEEEEECCHH
TSFFFDHYSAFVLGIDDSYAVADEGGGPRDLPPIRGHAALSQHIGASLMADEFDMSFFQD
HHHHHHHHEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHC
KPLDHGLFSPLSALLPWQNGWPMQVVPLQVGVLQFPIPSARRCYKLGQALRRAIESFPED
CCCCCHHHHHHHHHCCCCCCCCCEEEEEEECEEECCCCHHHHHHHHHHHHHHHHHHCCHH
LRVAVVATGGVSHQVHGERCGFNNPQWDEQFVDLLVNDPERLTEITLAEYATLGGLEGAE
EEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
VIMWLIMRGALSANVEKLHQAYYLPSMTGIATLILENQAREAPVDVHQRQRDKINLQLAG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEC
VEKLPGTYPFTHARSLKAIRINRFLHKLIQPAWRERFNNAQQALFDEAQLTTEEQQLLRE
HHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHH
LDWRGLIHYGVSFFLLEKLGAVVGVSNLHIYSAMRGQTLDEFQQTRNQQVLYSVAGKAPK
HCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCEEEHHHCCCCCC
>Mature Secondary Structure 
AKIIGGLAVSHTPTIGFAVDHNKQNETAWAPIFDGFAPMQQWLEEKKPDVLLYVFNDHV
CCCCCCEEECCCCCEEEEECCCCCCCCEECHHHCCHHHHHHHHHHCCCCEEEEEECCHH
TSFFFDHYSAFVLGIDDSYAVADEGGGPRDLPPIRGHAALSQHIGASLMADEFDMSFFQD
HHHHHHHHEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHC
KPLDHGLFSPLSALLPWQNGWPMQVVPLQVGVLQFPIPSARRCYKLGQALRRAIESFPED
CCCCCHHHHHHHHHCCCCCCCCCEEEEEEECEEECCCCHHHHHHHHHHHHHHHHHHCCHH
LRVAVVATGGVSHQVHGERCGFNNPQWDEQFVDLLVNDPERLTEITLAEYATLGGLEGAE
EEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
VIMWLIMRGALSANVEKLHQAYYLPSMTGIATLILENQAREAPVDVHQRQRDKINLQLAG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEC
VEKLPGTYPFTHARSLKAIRINRFLHKLIQPAWRERFNNAQQALFDEAQLTTEEQQLLRE
HHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHH
LDWRGLIHYGVSFFLLEKLGAVVGVSNLHIYSAMRGQTLDEFQQTRNQQVLYSVAGKAPK
HCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCEEEHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]