Click here to switch to the map view.

The map label for this gene is intE [H]

Identifier: 157370270

GI number: 157370270

Start: 2223258

End: 2224040

Strand: Reverse

Name: intE [H]

Synonym: Spro_2028

Alternate gene names: 157370270

Gene position: 2224040-2223258 (Counterclockwise)

Preceding gene: 157370273

Following gene: 157370266

Centisome position: 40.82

GC content: 47.38

Gene sequence:

>783_bases
GTGGACCTTCTACGCCAAAAATTTGCAATGAAATCCTTGCCAGAGGTTGATGCCAGAGACATCGCTTCGTTGTTGGATGA
GTACACAGATGCCGGACAGCCACGTATGGGTCAGGTTGTTCGCTCAGTGCTGATCGACGTATTCAAAGAGGCTCAGCATG
TTGGTGAGGTTCCGCCTGGCTACAATCCTGCGCTTGCCACAAAGCAACCACGCAGGAAGATCACGAGGCAGCGCTTGAGT
TTAGAGGAGTGGCAGAAGATTTTCGATATTGCTGATAAACAGCATCGGTACATGGGCAATGCAATGCTGTTGGCCGTAGT
TACAGGACAGCGCTTAGGTGACATTTCAGCAATGAAATTCAGCGATATCTGGGATGATCACTTACATGTTTTGCAGGAGA
AAACTGGTGCAAAGTTGGCACTGCCTCTTTCTCTTCGTTGCGATGCTTTGAACACAAGCTTACGTGAGGTTATCTCGCGT
TGCCGAGATTATGCTGTCAGTCAGTATCTTGTACATTACTTCCGTTCGACATCAATGGCACAACGTGGTGCCAAAGTACC
AGGAAATACGCTGACAACCAATTTTAGCAAAGCCAGGGATAAAACCGATATTGATTGGGGAGAAGGCACACCAGCAACGT
TTCATGAACAACGATCGCTTGCTGAAAGACTGTATAAGTCCCAAGGGGTAAACACACAGATACTTCTGGGCCACAAAACG
CAGTCTCAGACTGACCGTTATCATGATGACAGAGGGAAGGAGTGGATAAAAGTGAGTTGTTAG

Upstream 100 bases:

>100_bases
GAATTCTTCAGTGAAATTTTTGGCTGACATAAACACTCCTTCATTAAGCCTCTATTATGAGGCAAAAAAGTGTCTATAAA
ACCCGGGTCTATTCAAACCT

Downstream 100 bases:

>100_bases
CCTTATGGGGTGCTCTCAATAATTTGTTTATATTAGTTATTTTAGATTCTACTTAATTTGATATACTTTACGGTCTAGGC
TCGATCTGACCGTCAACTTA

Product: integrase family protein

Products: NA

Alternate protein names: Int(Lambda); Prophage e14 integrase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDLLRQKFAMKSLPEVDARDIASLLDEYTDAGQPRMGQVVRSVLIDVFKEAQHVGEVPPGYNPALATKQPRRKITRQRLS
LEEWQKIFDIADKQHRYMGNAMLLAVVTGQRLGDISAMKFSDIWDDHLHVLQEKTGAKLALPLSLRCDALNTSLREVISR
CRDYAVSQYLVHYFRSTSMAQRGAKVPGNTLTTNFSKARDKTDIDWGEGTPATFHEQRSLAERLYKSQGVNTQILLGHKT
QSQTDRYHDDRGKEWIKVSC

Sequences:

>Translated_260_residues
MDLLRQKFAMKSLPEVDARDIASLLDEYTDAGQPRMGQVVRSVLIDVFKEAQHVGEVPPGYNPALATKQPRRKITRQRLS
LEEWQKIFDIADKQHRYMGNAMLLAVVTGQRLGDISAMKFSDIWDDHLHVLQEKTGAKLALPLSLRCDALNTSLREVISR
CRDYAVSQYLVHYFRSTSMAQRGAKVPGNTLTTNFSKARDKTDIDWGEGTPATFHEQRSLAERLYKSQGVNTQILLGHKT
QSQTDRYHDDRGKEWIKVSC
>Mature_260_residues
MDLLRQKFAMKSLPEVDARDIASLLDEYTDAGQPRMGQVVRSVLIDVFKEAQHVGEVPPGYNPALATKQPRRKITRQRLS
LEEWQKIFDIADKQHRYMGNAMLLAVVTGQRLGDISAMKFSDIWDDHLHVLQEKTGAKLALPLSLRCDALNTSLREVISR
CRDYAVSQYLVHYFRSTSMAQRGAKVPGNTLTTNFSKARDKTDIDWGEGTPATFHEQRSLAERLYKSQGVNTQILLGHKT
QSQTDRYHDDRGKEWIKVSC

Specific function: Integrase from the cryptic lambdoic prophage e14. Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the h

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787386, Length=260, Percent_Identity=78.8461538461538, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016177
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR015094
- InterPro:   IPR023109
- InterPro:   IPR013087 [H]

Pfam domain/function: PF09003 Phage_integ_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 29619; Mature: 29619

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLRQKFAMKSLPEVDARDIASLLDEYTDAGQPRMGQVVRSVLIDVFKEAQHVGEVPPG
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
YNPALATKQPRRKITRQRLSLEEWQKIFDIADKQHRYMGNAMLLAVVTGQRLGDISAMKF
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHEEEEECCCHHCCCHHHHH
SDIWDDHLHVLQEKTGAKLALPLSLRCDALNTSLREVISRCRDYAVSQYLVHYFRSTSMA
HHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRGAKVPGNTLTTNFSKARDKTDIDWGEGTPATFHEQRSLAERLYKSQGVNTQILLGHKT
HCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
QSQTDRYHDDRGKEWIKVSC
HHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MDLLRQKFAMKSLPEVDARDIASLLDEYTDAGQPRMGQVVRSVLIDVFKEAQHVGEVPPG
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
YNPALATKQPRRKITRQRLSLEEWQKIFDIADKQHRYMGNAMLLAVVTGQRLGDISAMKF
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHEEEEECCCHHCCCHHHHH
SDIWDDHLHVLQEKTGAKLALPLSLRCDALNTSLREVISRCRDYAVSQYLVHYFRSTSMA
HHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRGAKVPGNTLTTNFSKARDKTDIDWGEGTPATFHEQRSLAERLYKSQGVNTQILLGHKT
HCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
QSQTDRYHDDRGKEWIKVSC
HHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]