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The map label for this gene is rluE [H]

Identifier: 157370266

GI number: 157370266

Start: 2217723

End: 2218259

Strand: Reverse

Name: rluE [H]

Synonym: Spro_2024

Alternate gene names: 157370266

Gene position: 2218259-2217723 (Counterclockwise)

Preceding gene: 157370270

Following gene: 157370265

Centisome position: 40.71

GC content: 55.87

Gene sequence:

>537_bases
GTGGTGTTATTCAATAAACCCTTCGATGTCCTGCCGCAATTTACCGATGAGGCCGGCCGCGCCACGCTGAAAGAATTCAT
TCCGTTTCCTGATGTTTACGCCGCCGGACGTCTGGATCGCGACAGCGAAGGATTATTGGTATTAACCAATGACGGCAAGC
TGCAGGCGCAGCTCACGGAGCCAGGTAAACGTACCGGCAAAGTGTATTACGTGCAGGTAGAAGGCGCGCCGCAGGACAAC
GATTTAGTCTCTTTACGTAACGGGGTTACGCTCAAAGACGGCCCGACGCTGCCGGCCGGTGTGGAGTTGGTGGCCGAACC
GCAGTGGCTATGGCCGCGAAATCCGCCGATCCGCGAGCGCAAGTCGATCCCCACCGCCTGGCTGAAAATCACCCTGTATG
AGGGACGAAACCGCCAGGTGCGTCGCATGACTGCACATATCGGCTTCCCTACCCTGCGGCTGATTCGCTACAGTATGGGT
AATATTTCATTGGGCGAACTGCAACCCGGTGAATGGAAATGCCTTGATTCGCTTTAA

Upstream 100 bases:

>100_bases
CTCAACTGTTATGAAAAAATTCCCTGTTAGAAATCACAAAGTTAAACGATTCAGCCAGCCTGCCTCCGCCAAACCCGCTG
TCGCACAGGGGCCGCGTCGC

Downstream 100 bases:

>100_bases
TCCGCCCGTCAATCCGTCGCTGTAAGGGGCCGCCATGTTTAAACCGCACGTTACCGTTGCCTGCGTGGTTCACGCCGCCG
GCAAGTTTTTAATCGTCGAA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MVLFNKPFDVLPQFTDEAGRATLKEFIPFPDVYAAGRLDRDSEGLLVLTNDGKLQAQLTEPGKRTGKVYYVQVEGAPQDN
DLVSLRNGVTLKDGPTLPAGVELVAEPQWLWPRNPPIRERKSIPTAWLKITLYEGRNRQVRRMTAHIGFPTLRLIRYSMG
NISLGELQPGEWKCLDSL

Sequences:

>Translated_178_residues
MVLFNKPFDVLPQFTDEAGRATLKEFIPFPDVYAAGRLDRDSEGLLVLTNDGKLQAQLTEPGKRTGKVYYVQVEGAPQDN
DLVSLRNGVTLKDGPTLPAGVELVAEPQWLWPRNPPIRERKSIPTAWLKITLYEGRNRQVRRMTAHIGFPTLRLIRYSMG
NISLGELQPGEWKCLDSL
>Mature_178_residues
MVLFNKPFDVLPQFTDEAGRATLKEFIPFPDVYAAGRLDRDSEGLLVLTNDGKLQAQLTEPGKRTGKVYYVQVEGAPQDN
DLVSLRNGVTLKDGPTLPAGVELVAEPQWLWPRNPPIRERKSIPTAWLKITLYEGRNRQVRRMTAHIGFPTLRLIRYSMG
NISLGELQPGEWKCLDSL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=173, Percent_Identity=80.9248554913295, Blast_Score=294, Evalue=2e-81,
Organism=Escherichia coli, GI1787524, Length=180, Percent_Identity=32.7777777777778, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1788510, Length=179, Percent_Identity=30.7262569832402, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1790453, Length=176, Percent_Identity=28.9772727272727, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20073; Mature: 20073

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLFNKPFDVLPQFTDEAGRATLKEFIPFPDVYAAGRLDRDSEGLLVLTNDGKLQAQLTE
CEEECCCHHHCCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCCCEEEEECCCEEEEEECC
PGKRTGKVYYVQVEGAPQDNDLVSLRNGVTLKDGPTLPAGVELVAEPQWLWPRNPPIRER
CCCCCCEEEEEEECCCCCCCCEEEECCCEEECCCCCCCCCEEEEECCCEECCCCCCCCHH
KSIPTAWLKITLYEGRNRQVRRMTAHIGFPTLRLIRYSMGNISLGELQPGEWKCLDSL
HCCCEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCEEECCCC
>Mature Secondary Structure
MVLFNKPFDVLPQFTDEAGRATLKEFIPFPDVYAAGRLDRDSEGLLVLTNDGKLQAQLTE
CEEECCCHHHCCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCCCEEEEECCCEEEEEECC
PGKRTGKVYYVQVEGAPQDNDLVSLRNGVTLKDGPTLPAGVELVAEPQWLWPRNPPIRER
CCCCCCEEEEEEECCCCCCCCEEEECCCEEECCCCCCCCCEEEEECCCEECCCCCCCCHH
KSIPTAWLKITLYEGRNRQVRRMTAHIGFPTLRLIRYSMGNISLGELQPGEWKCLDSL
HCCCEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]