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The map label for this gene is cobB [H]

Identifier: 157370247

GI number: 157370247

Start: 2195933

End: 2196769

Strand: Direct

Name: cobB [H]

Synonym: Spro_2005

Alternate gene names: 157370247

Gene position: 2195933-2196769 (Clockwise)

Preceding gene: 157370246

Following gene: 157370248

Centisome position: 40.3

GC content: 59.14

Gene sequence:

>837_bases
ATGCACACTCGCCATCGGCTGTGTCAGTTTCGCAAGAGTAAGCATGTGCTGCATCAGCGCTTTCGTTCGCGGATATTTCA
TCGCGACACCATGGCGGCGGCCGAGCTGAAAAAGCCGTTTGTCGTGGTGTTGACCGGGGCCGGGATCTCGGCCGAGTCGG
GTATTCGCACCTTCCGCGCTGCCGACGGGCTGTGGGAAGAGCACCGGGTAGAAGACGTTGCCACACCGGAAGGTTACCAG
CGCGATCCGCAGCTGGTTCAGACGTTTTACAATGAGCGTCGCCGCCAGCTGCAGTCGCCGGAGATTGCGCCTAATGCCGC
ACACCGTGCCCTGGCAGATCTCGAAGCCTGGCTGGGGGATAACTTCCTGCTGGTGACGCAGAATATCGATAATCTGCACG
AACGTGCCGGTAGCACCCGAGTGTTGCATATGCACGGCGAACTGCTGAAAGTGCGCTGCACTAGTTCCGGGCAGGTGTTT
GACTGGCCAGAAGATCTCAGCGTCGACGATCGCTGCCATTGCTGCCAGTTCCCGGCACCGTTGCGGCCACACATAGTATG
GTTCGGCGAAATGCCGCTGGGCATGGATGAAATTTATCAGGCGCTGGCCAAGGCCGACTTTTTCGTGGCTATCGGCACCT
CAGGCCACGTTTACCCGGCCGCCGGTTTTGTGCATGAGGCCCGGTTGGGCGGGGCGCATACCATGGAGCTGAATCTGGAA
CCCAGCCAGGTGGAGAGCCAGTTTGATGAAAAACACTATGGCCAGGCCAGCGCAGTGGTGCCACGGTTTGTGCATAAATT
TCTGGTCGGCAAGGTAGCACGAGCGGACCAGCCATAA

Upstream 100 bases:

>100_bases
AGTTGCCACGGATTGAAAAGGCGCGCTACGGCGATGCCGGTGGGGTCCGCGGAGCTGCGTTCCTCAATTTGGTCAACAGG
GAAAAGTAAGGAGAGAGTTA

Downstream 100 bases:

>100_bases
GTTTGACCCCAGGGGGCAAGTTTTTGCACCCTGGGGTTACACCCGCCGTTCCACGGGCTGACCATCCAGAAAGTACCCAC
GTTCTTTAGTCATAATAGCG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MHTRHRLCQFRKSKHVLHQRFRSRIFHRDTMAAAELKKPFVVVLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGYQ
RDPQLVQTFYNERRRQLQSPEIAPNAAHRALADLEAWLGDNFLLVTQNIDNLHERAGSTRVLHMHGELLKVRCTSSGQVF
DWPEDLSVDDRCHCCQFPAPLRPHIVWFGEMPLGMDEIYQALAKADFFVAIGTSGHVYPAAGFVHEARLGGAHTMELNLE
PSQVESQFDEKHYGQASAVVPRFVHKFLVGKVARADQP

Sequences:

>Translated_278_residues
MHTRHRLCQFRKSKHVLHQRFRSRIFHRDTMAAAELKKPFVVVLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGYQ
RDPQLVQTFYNERRRQLQSPEIAPNAAHRALADLEAWLGDNFLLVTQNIDNLHERAGSTRVLHMHGELLKVRCTSSGQVF
DWPEDLSVDDRCHCCQFPAPLRPHIVWFGEMPLGMDEIYQALAKADFFVAIGTSGHVYPAAGFVHEARLGGAHTMELNLE
PSQVESQFDEKHYGQASAVVPRFVHKFLVGKVARADQP
>Mature_278_residues
MHTRHRLCQFRKSKHVLHQRFRSRIFHRDTMAAAELKKPFVVVLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGYQ
RDPQLVQTFYNERRRQLQSPEIAPNAAHRALADLEAWLGDNFLLVTQNIDNLHERAGSTRVLHMHGELLKVRCTSSGQVF
DWPEDLSVDDRCHCCQFPAPLRPHIVWFGEMPLGMDEIYQALAKADFFVAIGTSGHVYPAAGFVHEARLGGAHTMELNLE
PSQVESQFDEKHYGQASAVVPRFVHKFLVGKVARADQP

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=248, Percent_Identity=37.5, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI6912664, Length=266, Percent_Identity=36.0902255639098, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI13787215, Length=254, Percent_Identity=37.007874015748, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI7657575, Length=268, Percent_Identity=29.4776119402985, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI308199517, Length=235, Percent_Identity=78.7234042553192, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17541892, Length=222, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71990482, Length=246, Percent_Identity=27.6422764227642, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17567771, Length=120, Percent_Identity=37.5, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 31523; Mature: 31523

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTRHRLCQFRKSKHVLHQRFRSRIFHRDTMAAAELKKPFVVVLTGAGISAESGIRTFRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
ADGLWEEHRVEDVATPEGYQRDPQLVQTFYNERRRQLQSPEIAPNAAHRALADLEAWLGD
HCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
NFLLVTQNIDNLHERAGSTRVLHMHGELLKVRCTSSGQVFDWPEDLSVDDRCHCCQFPAP
CEEEEECCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEECCCCCCCCCCCCCEECCCCC
LRPHIVWFGEMPLGMDEIYQALAKADFFVAIGTSGHVYPAAGFVHEARLGGAHTMELNLE
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCEECCCCHHHHHHCCCCEEEEECCC
PSQVESQFDEKHYGQASAVVPRFVHKFLVGKVARADQP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHTRHRLCQFRKSKHVLHQRFRSRIFHRDTMAAAELKKPFVVVLTGAGISAESGIRTFRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
ADGLWEEHRVEDVATPEGYQRDPQLVQTFYNERRRQLQSPEIAPNAAHRALADLEAWLGD
HCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
NFLLVTQNIDNLHERAGSTRVLHMHGELLKVRCTSSGQVFDWPEDLSVDDRCHCCQFPAP
CEEEEECCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEECCCCCCCCCCCCCEECCCCC
LRPHIVWFGEMPLGMDEIYQALAKADFFVAIGTSGHVYPAAGFVHEARLGGAHTMELNLE
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCEECCCCHHHHHHCCCCEEEEECCC
PSQVESQFDEKHYGQASAVVPRFVHKFLVGKVARADQP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]