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The map label for this gene is rob [H]

Identifier: 157370173

GI number: 157370173

Start: 2115133

End: 2115489

Strand: Direct

Name: rob [H]

Synonym: Spro_1931

Alternate gene names: 157370173

Gene position: 2115133-2115489 (Clockwise)

Preceding gene: 157370170

Following gene: 157370174

Centisome position: 38.82

GC content: 56.86

Gene sequence:

>357_bases
ATGAACAGCACGGGTTTTATTACTGATTTGATTGAGTGGATTGATAACAATCTGGAAGAGAAGCTGGATATCAACACCGT
CGCGGGCCGGGCGGGGTATTCCAAGTGGCACCTGCAGCGAATGTTCAAACGCCAGACCGGTTACGCGCTGGGAGAGTATA
TTCGCATGCAGAAGCTGAGGGTCTCGGCGGAGCGCCTGGCCAACAGCGGCGAACCGATCGTCAGCGTGGCGATCTCACTC
GGCTTTGACTCGCAGCAGTCCTTCAATCGCAGTTTTAAACGCCACTATGGTCAAACCCCGGGCGACTGGCGCCGTGGCGT
GATGCAGCCATCCATGGCGGCTGGCCACACGCATTGA

Upstream 100 bases:

>100_bases
TCTGTTAAATGCAATAATAAAAATTGCATTTAATGCGAAACTGCTGCAACGTAATGGCATCGGGCAGCGGATCTACCTGC
CAAGTCATGGGAGCGTCAGC

Downstream 100 bases:

>100_bases
GGCGGATTGCCGACAACAAAAAAGCCTGAACTTGCGTTCAGGCTTTCTCGTAAAATATGGCGGTGAGGGAGGGCTTGATT
CGCTGCGCTCACCCTGCGGG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MNSTGFITDLIEWIDNNLEEKLDINTVAGRAGYSKWHLQRMFKRQTGYALGEYIRMQKLRVSAERLANSGEPIVSVAISL
GFDSQQSFNRSFKRHYGQTPGDWRRGVMQPSMAAGHTH

Sequences:

>Translated_118_residues
MNSTGFITDLIEWIDNNLEEKLDINTVAGRAGYSKWHLQRMFKRQTGYALGEYIRMQKLRVSAERLANSGEPIVSVAISL
GFDSQQSFNRSFKRHYGQTPGDWRRGVMQPSMAAGHTH
>Mature_118_residues
MNSTGFITDLIEWIDNNLEEKLDINTVAGRAGYSKWHLQRMFKRQTGYALGEYIRMQKLRVSAERLANSGEPIVSVAISL
GFDSQQSFNRSFKRHYGQTPGDWRRGVMQPSMAAGHTH

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=105, Percent_Identity=48.5714285714286, Blast_Score=116, Evalue=4e-28,
Organism=Escherichia coli, GI87081928, Length=103, Percent_Identity=46.6019417475728, Blast_Score=110, Evalue=4e-26,
Organism=Escherichia coli, GI1790497, Length=104, Percent_Identity=45.1923076923077, Blast_Score=109, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 13462; Mature: 13462

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTGFITDLIEWIDNNLEEKLDINTVAGRAGYSKWHLQRMFKRQTGYALGEYIRMQKLR
CCCCHHHHHHHHHHHCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VSAERLANSGEPIVSVAISLGFDSQQSFNRSFKRHYGQTPGDWRRGVMQPSMAAGHTH
HHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNSTGFITDLIEWIDNNLEEKLDINTVAGRAGYSKWHLQRMFKRQTGYALGEYIRMQKLR
CCCCHHHHHHHHHHHCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VSAERLANSGEPIVSVAISLGFDSQQSFNRSFKRHYGQTPGDWRRGVMQPSMAAGHTH
HHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]