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The map label for this gene is holB [H]

Identifier: 157370154

GI number: 157370154

Start: 2095137

End: 2096144

Strand: Direct

Name: holB [H]

Synonym: Spro_1912

Alternate gene names: 157370154

Gene position: 2095137-2096144 (Clockwise)

Preceding gene: 157370153

Following gene: 157370155

Centisome position: 38.45

GC content: 59.23

Gene sequence:

>1008_bases
ATGAACTGGTACCCGTGGCTGAATACCCCTTACCGCCAGTTGGTCGGGCAATATGCTACCGGGCGCGGTCATCACGCACT
GTTGTTGCATGCCGCTGCCGGCCATGGTGAGGATGCGCTGGCCTATGGCTTGAGCCGTTGGCTGATCTGCCAACGTCGCA
ACGGTGAGAAAAGCTGCGGTGAATGCCATAGCTGTCGGTTGATGCTGGCAGGAAATCACCCGGATTATCACGTGCTGACG
CCGGAAAAAGGCAAAAGCAGCCTGGGGGTGGAACCCATCCGCCAGCTGATTGAGATCCTTTATTCTCATGCGCAGCAGGG
CGGTGCCAAGGTGATCTGGCTACCGCAGGCGGAGTTGCTGACCGAGGCTGCCGCCAATGCCCTGTTGAAAACGCTGGAAG
AACCGCCGGAAAAAACCTATTTCCTGCTGAGCTGTCGTGAGCCTTCACGCCTGCTGGCGACGCTGCGCAGCCGTTGTTTG
TACTGGCATCTCGCCAGTCCGGACGAACAGCTCAGTCTGCAATGGCTGAATCGGCAGACGCCGGGTAATCAGATCGATCT
CCTGACCGCCTTGCGCTTGCATGACGGCGCACCCTTGGCCGCAGAGCAATTGCTCCAGCCTGAACGTTGGCAACAACGTA
GCGCGCTGTGCGCGGCCCTGAGTGCCGCTTTGCCGCAGCACGACATGTTGTCGCTGCTGCCGGTATTGAATCACGAAGAC
GTCGCTGAGCGGCTACACTGGCTATGTTCGTTGTTGATGGACGCCATGAAATGGCAGCAGGGTGCCGCCGGCTTTGTTCT
GAACCAGGATCAGCAACCGCTGGTGCACCAGCTGGCCAGCCGCCTGAGCAGTACGTCGCTGCAGCAAGCCGTGCAGCAAT
GGCTGCATTGTCGCCATCAGTTGCTTAGCGTCGTCGGCGTTAACCGCGAACTGCTGCTGACCGAACAGTTACTTGGCTGG
GAGCAGTTGCTCGTCGCTACCGGCTATTCCCACCCTCATTCGCTGTAA

Upstream 100 bases:

>100_bases
CGTCCATCATCACCGTAGATGCTTCTCAATCGCTTGATCAGGTGACGGCGGCGATTCGTCGTTGCGTCAGCCAATGGTTA
CAGCAGCAGGAAGGCGCGTA

Downstream 100 bases:

>100_bases
AAGAGTTAAATATTATGTTGTTAGTAGATTCTCATTGTCACCTCGACGGCCTGGATTACCAAACTCTGCATCAAAATGTG
GATGATGCTTTGGCGAAGGC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNWYPWLNTPYRQLVGQYATGRGHHALLLHAAAGHGEDALAYGLSRWLICQRRNGEKSCGECHSCRLMLAGNHPDYHVLT
PEKGKSSLGVEPIRQLIEILYSHAQQGGAKVIWLPQAELLTEAAANALLKTLEEPPEKTYFLLSCREPSRLLATLRSRCL
YWHLASPDEQLSLQWLNRQTPGNQIDLLTALRLHDGAPLAAEQLLQPERWQQRSALCAALSAALPQHDMLSLLPVLNHED
VAERLHWLCSLLMDAMKWQQGAAGFVLNQDQQPLVHQLASRLSSTSLQQAVQQWLHCRHQLLSVVGVNRELLLTEQLLGW
EQLLVATGYSHPHSL

Sequences:

>Translated_335_residues
MNWYPWLNTPYRQLVGQYATGRGHHALLLHAAAGHGEDALAYGLSRWLICQRRNGEKSCGECHSCRLMLAGNHPDYHVLT
PEKGKSSLGVEPIRQLIEILYSHAQQGGAKVIWLPQAELLTEAAANALLKTLEEPPEKTYFLLSCREPSRLLATLRSRCL
YWHLASPDEQLSLQWLNRQTPGNQIDLLTALRLHDGAPLAAEQLLQPERWQQRSALCAALSAALPQHDMLSLLPVLNHED
VAERLHWLCSLLMDAMKWQQGAAGFVLNQDQQPLVHQLASRLSSTSLQQAVQQWLHCRHQLLSVVGVNRELLLTEQLLGW
EQLLVATGYSHPHSL
>Mature_335_residues
MNWYPWLNTPYRQLVGQYATGRGHHALLLHAAAGHGEDALAYGLSRWLICQRRNGEKSCGECHSCRLMLAGNHPDYHVLT
PEKGKSSLGVEPIRQLIEILYSHAQQGGAKVIWLPQAELLTEAAANALLKTLEEPPEKTYFLLSCREPSRLLATLRSRCL
YWHLASPDEQLSLQWLNRQTPGNQIDLLTALRLHDGAPLAAEQLLQPERWQQRSALCAALSAALPQHDMLSLLPVLNHED
VAERLHWLCSLLMDAMKWQQGAAGFVLNQDQQPLVHQLASRLSSTSLQQAVQQWLHCRHQLLSVVGVNRELLLTEQLLGW
EQLLVATGYSHPHSL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=324, Percent_Identity=54.9382716049383, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1786676, Length=148, Percent_Identity=36.4864864864865, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37699; Mature: 37699

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWYPWLNTPYRQLVGQYATGRGHHALLLHAAAGHGEDALAYGLSRWLICQRRNGEKSCG
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCC
ECHSCRLMLAGNHPDYHVLTPEKGKSSLGVEPIRQLIEILYSHAQQGGAKVIWLPQAELL
CHHHCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TEAAANALLKTLEEPPEKTYFLLSCREPSRLLATLRSRCLYWHLASPDEQLSLQWLNRQT
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCC
PGNQIDLLTALRLHDGAPLAAEQLLQPERWQQRSALCAALSAALPQHDMLSLLPVLNHED
CCCHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
VAERLHWLCSLLMDAMKWQQGAAGFVLNQDQQPLVHQLASRLSSTSLQQAVQQWLHCRHQ
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSVVGVNRELLLTEQLLGWEQLLVATGYSHPHSL
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNWYPWLNTPYRQLVGQYATGRGHHALLLHAAAGHGEDALAYGLSRWLICQRRNGEKSCG
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCC
ECHSCRLMLAGNHPDYHVLTPEKGKSSLGVEPIRQLIEILYSHAQQGGAKVIWLPQAELL
CHHHCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TEAAANALLKTLEEPPEKTYFLLSCREPSRLLATLRSRCLYWHLASPDEQLSLQWLNRQT
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCC
PGNQIDLLTALRLHDGAPLAAEQLLQPERWQQRSALCAALSAALPQHDMLSLLPVLNHED
CCCHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
VAERLHWLCSLLMDAMKWQQGAAGFVLNQDQQPLVHQLASRLSSTSLQQAVQQWLHCRHQ
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSVVGVNRELLLTEQLLGWEQLLVATGYSHPHSL
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164 [H]