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The map label for this gene is eptB [H]

Identifier: 157370101

GI number: 157370101

Start: 2041985

End: 2043649

Strand: Direct

Name: eptB [H]

Synonym: Spro_1859

Alternate gene names: 157370101

Gene position: 2041985-2043649 (Clockwise)

Preceding gene: 157370100

Following gene: 157370106

Centisome position: 37.48

GC content: 51.23

Gene sequence:

>1665_bases
ATGGTATCAATAATGCAGAAGTTCCGACGCTGGACCCAGTTGCAGGTCTCTTTCTTTATCGCTTTTTATCTCGGTGTGTT
GCTCAATATCCCCATATTTTACCGCCGTTATCAGCAACTTCATTATGACAACTCCTTGTCGATCGGCATTGAGGTGCTCG
CAGCCTTCTGCCTGGTGTGTTTTATCACGCTACTGATCTCTTTTTTGGGGCGCAGGCTGTTCCGTGTTTTGGCATCGTTA
TTGGTGCTGAGTTCGGTTGCCGCCAGCTACTACATGGTGTTATTCCACGTTGATATTGGCTACGGCATTCTGGCGGCGGT
GATGACTACTGACGTCGATCTTTCCAAGGAGTCGGTGGGTTGGCATTTTGGCGTGTGGGTGGTGCTGGTGAGTCTGTTGC
CGTTACTGCTGATTTGGCTGGCGCCGATGCATGAGGCCAGTTATAGGCGCGCTAACCATCTGGCATGGTACAAAAAAGGT
GGCGTGATGCTGACCGCCGGGCTGCTGTGCTGGCTACCCCTGAAGCTAATGGGGCAGGTACAGGATCGTCACGATCGGGA
AAATAATCAAATGATGGCCAGCTATGGCGGGGTGGTGGCCGGTAGTTATTCACCGTCCAATTGGCTCTCGGCCTTGGGGT
TGTTGACTTACAGCTCATACAGCCAGGCAGAAGACAGCCGTAATCTGTTTGATCCGGCGGCGCACTTTACTTATCAACCG
CCAAAAGACGTGAATGATCTGTATGTGGTGTTTGTGATTGGTGAGAGTGCCAGACGCGATCATATGGGCATTTACGGCTA
CCAGCGCGACAATACGCCTAACCTGGATAAGGAAACCAACCTTGCGGTGCTGCAGGGCTATTCCTGCGACACCTCGACCA
AGCTCTCGCTACGATGCATGTTTGTGAGAGAAGGTGGTGCGTCGGAAGAACCACAGCGGACCCTGAAGGAAATGAACGTA
TTTTCCGTGCTGAAAAAGCAGGGTTTTTCCTCGGAGCTGTTTTCAATGCAGAGCGAAGCCTGGTTTTACAACAAAACCCA
GGCGGATGATTACGCGCTGAGAGAAAGCATCCAGGCGGAAAAACGCAATGCCGGCAAACCCGTGGATGATATTGCGTTGA
TTGACGAGTTAAAAGATTCGTTAGACCGCCACCCGCAGGGCAAGCATTTGGTGATCCTGCATACCAAAGGCTCGCACTAT
ATGTACACCGAGCGCTATCCCCGCGCTTTCGCCCGTTACCAGCCGGAATGTCAGGGTATTGATGACACTTGCTCTACGCA
ATCAATGATTAACTCTTACGACAACAGTCTGCTGTATACCGATTATGTACTCGAGCAGGTTTTCAATCAGTTGCGCGATC
GTAATGCCATCGTGTTTTACGCCTCGGATCACGGCGAGTCGATCTCGGAAAATATGCATTTCCACGGTACCCCGCGTGAC
CATGCGCCGCAGGCGCAGCGCACCATTCCTATTATGGTTTGGGCATCTGACAAGTTTCTTAGCCAGCCCGACCATCAGGC
GGCCTTTAATCAGCTAAAAGCACTGCAGAAGGCAAAAACGCCGGTATTCCACGAGAAACTGTTCGACAGTATTCTCGGCT
GTATTGGTTATACCTCACCGGATGGCGGTATTGTGAAACAGCATAACTGGTGCCAGGTTAACTAA

Upstream 100 bases:

>100_bases
GCCTAAATTGCCTGTCAGATAACCATCCAGCAGCAGGAGAGAGAAAACTCTCAACAATAACCTGTATCTGGCAAACGGTT
ATGAATTCACTTTGGCAAGA

Downstream 100 bases:

>100_bases
ACCAACCGCCAGGGATGGGGTACCAGGAGCACATTTTCCGCAATAAAAAAGCCACCTCTTCAAGAGGTGGCTTTTTACTA
GAGCATGGTTTTAGTCCAGT

Product: phosphoethanolamine transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MVSIMQKFRRWTQLQVSFFIAFYLGVLLNIPIFYRRYQQLHYDNSLSIGIEVLAAFCLVCFITLLISFLGRRLFRVLASL
LVLSSVAASYYMVLFHVDIGYGILAAVMTTDVDLSKESVGWHFGVWVVLVSLLPLLLIWLAPMHEASYRRANHLAWYKKG
GVMLTAGLLCWLPLKLMGQVQDRHDRENNQMMASYGGVVAGSYSPSNWLSALGLLTYSSYSQAEDSRNLFDPAAHFTYQP
PKDVNDLYVVFVIGESARRDHMGIYGYQRDNTPNLDKETNLAVLQGYSCDTSTKLSLRCMFVREGGASEEPQRTLKEMNV
FSVLKKQGFSSELFSMQSEAWFYNKTQADDYALRESIQAEKRNAGKPVDDIALIDELKDSLDRHPQGKHLVILHTKGSHY
MYTERYPRAFARYQPECQGIDDTCSTQSMINSYDNSLLYTDYVLEQVFNQLRDRNAIVFYASDHGESISENMHFHGTPRD
HAPQAQRTIPIMVWASDKFLSQPDHQAAFNQLKALQKAKTPVFHEKLFDSILGCIGYTSPDGGIVKQHNWCQVN

Sequences:

>Translated_554_residues
MVSIMQKFRRWTQLQVSFFIAFYLGVLLNIPIFYRRYQQLHYDNSLSIGIEVLAAFCLVCFITLLISFLGRRLFRVLASL
LVLSSVAASYYMVLFHVDIGYGILAAVMTTDVDLSKESVGWHFGVWVVLVSLLPLLLIWLAPMHEASYRRANHLAWYKKG
GVMLTAGLLCWLPLKLMGQVQDRHDRENNQMMASYGGVVAGSYSPSNWLSALGLLTYSSYSQAEDSRNLFDPAAHFTYQP
PKDVNDLYVVFVIGESARRDHMGIYGYQRDNTPNLDKETNLAVLQGYSCDTSTKLSLRCMFVREGGASEEPQRTLKEMNV
FSVLKKQGFSSELFSMQSEAWFYNKTQADDYALRESIQAEKRNAGKPVDDIALIDELKDSLDRHPQGKHLVILHTKGSHY
MYTERYPRAFARYQPECQGIDDTCSTQSMINSYDNSLLYTDYVLEQVFNQLRDRNAIVFYASDHGESISENMHFHGTPRD
HAPQAQRTIPIMVWASDKFLSQPDHQAAFNQLKALQKAKTPVFHEKLFDSILGCIGYTSPDGGIVKQHNWCQVN
>Mature_554_residues
MVSIMQKFRRWTQLQVSFFIAFYLGVLLNIPIFYRRYQQLHYDNSLSIGIEVLAAFCLVCFITLLISFLGRRLFRVLASL
LVLSSVAASYYMVLFHVDIGYGILAAVMTTDVDLSKESVGWHFGVWVVLVSLLPLLLIWLAPMHEASYRRANHLAWYKKG
GVMLTAGLLCWLPLKLMGQVQDRHDRENNQMMASYGGVVAGSYSPSNWLSALGLLTYSSYSQAEDSRNLFDPAAHFTYQP
PKDVNDLYVVFVIGESARRDHMGIYGYQRDNTPNLDKETNLAVLQGYSCDTSTKLSLRCMFVREGGASEEPQRTLKEMNV
FSVLKKQGFSSELFSMQSEAWFYNKTQADDYALRESIQAEKRNAGKPVDDIALIDELKDSLDRHPQGKHLVILHTKGSHY
MYTERYPRAFARYQPECQGIDDTCSTQSMINSYDNSLLYTDYVLEQVFNQLRDRNAIVFYASDHGESISENMHFHGTPRD
HAPQAQRTIPIMVWASDKFLSQPDHQAAFNQLKALQKAKTPVFHEKLFDSILGCIGYTSPDGGIVKQHNWCQVN

Specific function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases dia

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082286, Length=560, Percent_Identity=51.6071428571429, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI87082372, Length=550, Percent_Identity=26.5454545454545, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082223, Length=252, Percent_Identity=32.5396825396825, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790392, Length=495, Percent_Identity=23.2323232323232, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787035, Length=258, Percent_Identity=28.2945736434109, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 63173; Mature: 63173

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIMQKFRRWTQLQVSFFIAFYLGVLLNIPIFYRRYQQLHYDNSLSIGIEVLAAFCLVC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FITLLISFLGRRLFRVLASLLVLSSVAASYYMVLFHVDIGYGILAAVMTTDVDLSKESVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEEEHHHHHHHHHHHHCCCCCCHHHCC
WHFGVWVVLVSLLPLLLIWLAPMHEASYRRANHLAWYKKGGVMLTAGLLCWLPLKLMGQV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEECCCEEEHHHHHHHHHHHHHHHH
QDRHDRENNQMMASYGGVVAGSYSPSNWLSALGLLTYSSYSQAEDSRNLFDPAAHFTYQP
HHHHCCCCCCEEHHCCCEEECCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHHCCCCCC
PKDVNDLYVVFVIGESARRDHMGIYGYQRDNTPNLDKETNLAVLQGYSCDTSTKLSLRCM
CCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEE
FVREGGASEEPQRTLKEMNVFSVLKKQGFSSELFSMQSEAWFYNKTQADDYALRESIQAE
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
KRNAGKPVDDIALIDELKDSLDRHPQGKHLVILHTKGSHYMYTERYPRAFARYQPECQGI
HHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCCCCCC
DDTCSTQSMINSYDNSLLYTDYVLEQVFNQLRDRNAIVFYASDHGESISENMHFHGTPRD
CCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCC
HAPQAQRTIPIMVWASDKFLSQPDHQAAFNQLKALQKAKTPVFHEKLFDSILGCIGYTSP
CCCCCCCCEEEEEEECCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
DGGIVKQHNWCQVN
CCCEEECCCCEECC
>Mature Secondary Structure
MVSIMQKFRRWTQLQVSFFIAFYLGVLLNIPIFYRRYQQLHYDNSLSIGIEVLAAFCLVC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FITLLISFLGRRLFRVLASLLVLSSVAASYYMVLFHVDIGYGILAAVMTTDVDLSKESVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEEEHHHHHHHHHHHHCCCCCCHHHCC
WHFGVWVVLVSLLPLLLIWLAPMHEASYRRANHLAWYKKGGVMLTAGLLCWLPLKLMGQV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEECCCEEEHHHHHHHHHHHHHHHH
QDRHDRENNQMMASYGGVVAGSYSPSNWLSALGLLTYSSYSQAEDSRNLFDPAAHFTYQP
HHHHCCCCCCEEHHCCCEEECCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHHCCCCCC
PKDVNDLYVVFVIGESARRDHMGIYGYQRDNTPNLDKETNLAVLQGYSCDTSTKLSLRCM
CCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEE
FVREGGASEEPQRTLKEMNVFSVLKKQGFSSELFSMQSEAWFYNKTQADDYALRESIQAE
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
KRNAGKPVDDIALIDELKDSLDRHPQGKHLVILHTKGSHYMYTERYPRAFARYQPECQGI
HHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCCCCCC
DDTCSTQSMINSYDNSLLYTDYVLEQVFNQLRDRNAIVFYASDHGESISENMHFHGTPRD
CCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCC
HAPQAQRTIPIMVWASDKFLSQPDHQAAFNQLKALQKAKTPVFHEKLFDSILGCIGYTSP
CCCCCCCCEEEEEEECCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
DGGIVKQHNWCQVN
CCCEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9298646 [H]