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The map label for this gene is rlmL

Identifier: 157369986

GI number: 157369986

Start: 1920060

End: 1922180

Strand: Direct

Name: rlmL

Synonym: Spro_1743

Alternate gene names: 157369986

Gene position: 1920060-1922180 (Clockwise)

Preceding gene: 157369984

Following gene: 157369987

Centisome position: 35.24

GC content: 56.01

Gene sequence:

>2121_bases
ATGAACTCTCTGTTTGCCAGCACGGCGCGTGGACTGGAAGAACTGTTAAAAAGCGAACTCGAAGCGCTGGGCGCTCACGC
CTGTAAAGTCGTACAGGGTGGGGTACATTTTCAGGGTGACGATCGTCTTCTGTACCAAAGCTTGCTGTGGAGCCGTTTGG
CCTCACGTATTTTGCTGCCGTTGAATGAATTCCGCGTACACAGCGATCTGGATTTGTACCTGGGCGTACAGGCGATCGAC
TGGACGAGCATTTTTGGTGTGGATAAAACTTTCGCCGTTCACTTCAGTGGCGTTAACGAAGAGATCCGCAACAGCCAGTA
TGGCGCACTGAAGGTCAAGGACGCCATTGTCGACAGTTTTACCCGCAAGATTGAACAACGCCCGACGGTGGCGAAGCAGC
AGCCGGACATTCGCGTCAATGTATTCCTGCAGCGCGATATGGCCAGCGTAGCGCTGGATTTGAGCGGTGAAGGCCTGCAC
CAGCGTGGTTATCGTGATCTGACCGGACAGGCACCGCTGAAAGAAAGCCTGGCAGCGGCGATTGTACAGCGTTCTGGCTG
GCAGCCGGGCACGCCAATGCTGGATCCAATGTGTGGTTCGGGTACCCTGCTGATCGAAGCGGCGATGATTGCTTCCGATC
GCGCACCTGGCCTGCATCGTCAACACTGGGGCTTTACCGCCTGGAATGGTCACAACGTCGATCTGTGGCGTGAAGTGATC
ACCGAAGCGCAGGTACGCGCCCGTCGTGGCCTGCAGGAAACCACCTCGCGTTTCTTCGGCTCCGATATTGATCGCCGGGT
GATTGAAATGGCACGCGGTAACGCCCGTCGTGCCGGCGTCGCTGAACTGATTACCTTTAACGTCAACGACGTAGCCAAAC
TGACCAACCCGCTGCCGGAAGGCCCGACGGGAACCGTAGTCTGCAACCCGCCATACGGCGAGCGTCTGGAAAGCGAACCG
GCGTTGATTGCCTTGCATAATATGCTGGGTCGCATCATGAAAAGCGCTTTTGGTGGCTGGCAGTTGTCACTGTTCAGCGC
CTCACCGGAGCTGTTGAGCTGCCTGCAACTGCGTGCCGAGCGCCAGTTCAAGGCAAAAAATGGCCCGTTGGACTGCGTGC
AGAAAAATTACCAACTGGCAGAAAACCCGCTGGGTGCCGGTGGCGTACAGGTGGCGGAAGATTTTGCCAACCGTCTGCGC
AAAAACCTGAAGAAGCTCGACAAGTGGGCCAAACAGCAGGGCATTGAGTGCTACCGCCTGTACGATGCTGACCTGCCGGA
ATATAACGTGGCGGTGGATCGCTACGGCAGCAAAGTCGTGGTGCAGGAATATGCGCCGCCGAAAACCATTGATGCGCAGA
AGGCTCGTCAACGCCTGTTCGACGTGATCAACGCCACGTTGGCGGTGCTGGAATTGCCGTCCAACCAGTTGATCCTGAAA
ACCCGCGAACGCCAGAAAGGTAAAAACCAGTACGAAAAACTGGCGCAGAAGGGCGAATTCCTGCTGGTGGAAGAGTTCAA
CGCCAAGCTGTGGGTTAACCTGACGGACTATCTCGATACCGGTCTGTTCCTCGACCACCGTATTGCCCGCCGCATGTTGG
GGGAGATGAGCAAAGGCAAAGACTTCCTCAACCTGTTTGCCTATACCGGCACCGCCAGCGTGCATGCCGGGCTGGGTGGG
GCGCGCAGCACCACGACGGTGGATATGTCGCGCACCTATCTGGAATGGGCAGAGAAAAACCTGCGGGTTAACGGCCTGAC
CGGCAAACAGCACAGATTGATCCATGCGGATTGTCTGTCGTGGATGCAAAATGCCGACGAACAATTCGATGTGATCTTTA
TCGATCCGCCGACCTTCTCCAACTCCAAGCGGATGGAGAACACTTTTGACGTTCAGCGCGATCACCTGGCGCTGATGCAA
GATTTGAAACGGTTACTGCGTCGTAACGGGACCATTATGTTCTCGAACAACAAGCGCGGTTTCCAGATGGATATGGCTGG
CCTGAGCGCATTGGGTCTGGAGGCGAAAGAAATTACCGCCCAGACGCAGTCGCAGGACTTTGCCCGTAACCGTCAAATTC
ATAACTGCTGGCTGCTTACCCATGCCGGCGAAGGAAAATAA

Upstream 100 bases:

>100_bases
TGTCTCCGCGCGATTAATAAATAGAAGGGAGCAACTTTATGACAAGCGGTCTGGATTAGCTATAATGCGCAACAATTTTC
TTTTTGGTGATAAGTACGAT

Downstream 100 bases:

>100_bases
TTACTATGTCGTTAATTAGCATGTCCGGTGCCTGGCTGTCCTTCAGCGATGCACCACTTTTAGACAACACCGAAATTCAT
ATTGAAGACAACGAACGCGT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MNSLFASTARGLEELLKSELEALGAHACKVVQGGVHFQGDDRLLYQSLLWSRLASRILLPLNEFRVHSDLDLYLGVQAID
WTSIFGVDKTFAVHFSGVNEEIRNSQYGALKVKDAIVDSFTRKIEQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLH
QRGYRDLTGQAPLKESLAAAIVQRSGWQPGTPMLDPMCGSGTLLIEAAMIASDRAPGLHRQHWGFTAWNGHNVDLWREVI
TEAQVRARRGLQETTSRFFGSDIDRRVIEMARGNARRAGVAELITFNVNDVAKLTNPLPEGPTGTVVCNPPYGERLESEP
ALIALHNMLGRIMKSAFGGWQLSLFSASPELLSCLQLRAERQFKAKNGPLDCVQKNYQLAENPLGAGGVQVAEDFANRLR
KNLKKLDKWAKQQGIECYRLYDADLPEYNVAVDRYGSKVVVQEYAPPKTIDAQKARQRLFDVINATLAVLELPSNQLILK
TRERQKGKNQYEKLAQKGEFLLVEEFNAKLWVNLTDYLDTGLFLDHRIARRMLGEMSKGKDFLNLFAYTGTASVHAGLGG
ARSTTTVDMSRTYLEWAEKNLRVNGLTGKQHRLIHADCLSWMQNADEQFDVIFIDPPTFSNSKRMENTFDVQRDHLALMQ
DLKRLLRRNGTIMFSNNKRGFQMDMAGLSALGLEAKEITAQTQSQDFARNRQIHNCWLLTHAGEGK

Sequences:

>Translated_706_residues
MNSLFASTARGLEELLKSELEALGAHACKVVQGGVHFQGDDRLLYQSLLWSRLASRILLPLNEFRVHSDLDLYLGVQAID
WTSIFGVDKTFAVHFSGVNEEIRNSQYGALKVKDAIVDSFTRKIEQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLH
QRGYRDLTGQAPLKESLAAAIVQRSGWQPGTPMLDPMCGSGTLLIEAAMIASDRAPGLHRQHWGFTAWNGHNVDLWREVI
TEAQVRARRGLQETTSRFFGSDIDRRVIEMARGNARRAGVAELITFNVNDVAKLTNPLPEGPTGTVVCNPPYGERLESEP
ALIALHNMLGRIMKSAFGGWQLSLFSASPELLSCLQLRAERQFKAKNGPLDCVQKNYQLAENPLGAGGVQVAEDFANRLR
KNLKKLDKWAKQQGIECYRLYDADLPEYNVAVDRYGSKVVVQEYAPPKTIDAQKARQRLFDVINATLAVLELPSNQLILK
TRERQKGKNQYEKLAQKGEFLLVEEFNAKLWVNLTDYLDTGLFLDHRIARRMLGEMSKGKDFLNLFAYTGTASVHAGLGG
ARSTTTVDMSRTYLEWAEKNLRVNGLTGKQHRLIHADCLSWMQNADEQFDVIFIDPPTFSNSKRMENTFDVQRDHLALMQ
DLKRLLRRNGTIMFSNNKRGFQMDMAGLSALGLEAKEITAQTQSQDFARNRQIHNCWLLTHAGEGK
>Mature_706_residues
MNSLFASTARGLEELLKSELEALGAHACKVVQGGVHFQGDDRLLYQSLLWSRLASRILLPLNEFRVHSDLDLYLGVQAID
WTSIFGVDKTFAVHFSGVNEEIRNSQYGALKVKDAIVDSFTRKIEQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLH
QRGYRDLTGQAPLKESLAAAIVQRSGWQPGTPMLDPMCGSGTLLIEAAMIASDRAPGLHRQHWGFTAWNGHNVDLWREVI
TEAQVRARRGLQETTSRFFGSDIDRRVIEMARGNARRAGVAELITFNVNDVAKLTNPLPEGPTGTVVCNPPYGERLESEP
ALIALHNMLGRIMKSAFGGWQLSLFSASPELLSCLQLRAERQFKAKNGPLDCVQKNYQLAENPLGAGGVQVAEDFANRLR
KNLKKLDKWAKQQGIECYRLYDADLPEYNVAVDRYGSKVVVQEYAPPKTIDAQKARQRLFDVINATLAVLELPSNQLILK
TRERQKGKNQYEKLAQKGEFLLVEEFNAKLWVNLTDYLDTGLFLDHRIARRMLGEMSKGKDFLNLFAYTGTASVHAGLGG
ARSTTTVDMSRTYLEWAEKNLRVNGLTGKQHRLIHADCLSWMQNADEQFDVIFIDPPTFSNSKRMENTFDVQRDHLALMQ
DLKRLLRRNGTIMFSNNKRGFQMDMAGLSALGLEAKEITAQTQSQDFARNRQIHNCWLLTHAGEGK

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=702, Percent_Identity=76.3532763532764, Blast_Score=1117, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=243, Percent_Identity=28.3950617283951, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_SERP5 (A8GCK7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477975.1
- ProteinModelPortal:   A8GCK7
- SMR:   A8GCK7
- STRING:   A8GCK7
- GeneID:   5604782
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1743
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   SPRO399741:SPRO_1743-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 79304; Mature: 79304

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLFASTARGLEELLKSELEALGAHACKVVQGGVHFQGDDRLLYQSLLWSRLASRILLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCC
LNEFRVHSDLDLYLGVQAIDWTSIFGVDKTFAVHFSGVNEEIRNSQYGALKVKDAIVDSF
HHHHEECCCCEEEEEEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCEEEHHHHHHHHH
TRKIEQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLHQRGYRDLTGQAPLKESLAAA
HHHHHHCCCCCCCCCCCEEEEEEECCHHHHEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
IVQRSGWQPGTPMLDPMCGSGTLLIEAAMIASDRAPGLHRQHWGFTAWNGHNVDLWREVI
HHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHH
TEAQVRARRGLQETTSRFFGSDIDRRVIEMARGNARRAGVAELITFNVNDVAKLTNPLPE
HHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHEEEECCHHHHHHCCCCCC
GPTGTVVCNPPYGERLESEPALIALHNMLGRIMKSAFGGWQLSLFSASPELLSCLQLRAE
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
RQFKAKNGPLDCVQKNYQLAENPLGAGGVQVAEDFANRLRKNLKKLDKWAKQQGIECYRL
HHHHCCCCCHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYGSKVVVQEYAPPKTIDAQKARQRLFDVINATLAVLELPSNQLILK
ECCCCCCCCCHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEE
TRERQKGKNQYEKLAQKGEFLLVEEFNAKLWVNLTDYLDTGLFLDHRIARRMLGEMSKGK
CHHHHCCHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCC
DFLNLFAYTGTASVHAGLGGARSTTTVDMSRTYLEWAEKNLRVNGLTGKQHRLIHADCLS
HHHHHHHCCCCCHHCCCCCCCCCCEEEHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
WMQNADEQFDVIFIDPPTFSNSKRMENTFDVQRDHLALMQDLKRLLRRNGTIMFSNNKRG
HHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
FQMDMAGLSALGLEAKEITAQTQSQDFARNRQIHNCWLLTHAGEGK
EEEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC
>Mature Secondary Structure
MNSLFASTARGLEELLKSELEALGAHACKVVQGGVHFQGDDRLLYQSLLWSRLASRILLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCC
LNEFRVHSDLDLYLGVQAIDWTSIFGVDKTFAVHFSGVNEEIRNSQYGALKVKDAIVDSF
HHHHEECCCCEEEEEEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCEEEHHHHHHHHH
TRKIEQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLHQRGYRDLTGQAPLKESLAAA
HHHHHHCCCCCCCCCCCEEEEEEECCHHHHEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
IVQRSGWQPGTPMLDPMCGSGTLLIEAAMIASDRAPGLHRQHWGFTAWNGHNVDLWREVI
HHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHH
TEAQVRARRGLQETTSRFFGSDIDRRVIEMARGNARRAGVAELITFNVNDVAKLTNPLPE
HHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHEEEECCHHHHHHCCCCCC
GPTGTVVCNPPYGERLESEPALIALHNMLGRIMKSAFGGWQLSLFSASPELLSCLQLRAE
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
RQFKAKNGPLDCVQKNYQLAENPLGAGGVQVAEDFANRLRKNLKKLDKWAKQQGIECYRL
HHHHCCCCCHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYGSKVVVQEYAPPKTIDAQKARQRLFDVINATLAVLELPSNQLILK
ECCCCCCCCCHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEE
TRERQKGKNQYEKLAQKGEFLLVEEFNAKLWVNLTDYLDTGLFLDHRIARRMLGEMSKGK
CHHHHCCHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCC
DFLNLFAYTGTASVHAGLGGARSTTTVDMSRTYLEWAEKNLRVNGLTGKQHRLIHADCLS
HHHHHHHCCCCCHHCCCCCCCCCCEEEHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
WMQNADEQFDVIFIDPPTFSNSKRMENTFDVQRDHLALMQDLKRLLRRNGTIMFSNNKRG
HHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
FQMDMAGLSALGLEAKEITAQTQSQDFARNRQIHNCWLLTHAGEGK
EEEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA