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The map label for this gene is ansB [H]

Identifier: 157369948

GI number: 157369948

Start: 1871554

End: 1872600

Strand: Direct

Name: ansB [H]

Synonym: Spro_1705

Alternate gene names: 157369948

Gene position: 1871554-1872600 (Clockwise)

Preceding gene: 157369943

Following gene: 157369949

Centisome position: 34.35

GC content: 55.49

Gene sequence:

>1047_bases
ATGAAATCAGTGAAACTCAGCGTTGTAGCGCTACTGCTGGCGGGGGCAAGCGGTTCTGCGTTGGCATTGCCCACCATTAC
CCTGTTGGCGACCGGCGGCACCATTGCCGGTGGCGGCGAGTCGGCGACAAAATCCAACTATACGGCGGGCAAACTGGGCG
TTGATGCTCTGGTGAATGCCGTACCGGAGCTGAAAAACGTCGCCAATATCCAGGGTGAGCAGGTGGTAAACATCGGTTCG
CAGGATATGAATGATGAGGTTTGGCTGAAGCTGGCGAAGAAAATCAACGCCGACTGCGCTAAAACGGACGGTTTTGTTAT
CACCCATGGCACCGACACGCTGGAAGAAACCGCCTACTTTCTTGATCTGACGGTTAAATGTGACAAACCGGTGGTGATTG
TCGGGGCGATGCGCCCGGCCACGGCGATGAGTGCCGACGGTCCGTTTAATCTGTATAACGCCGTAGTGACGGCGGCAGAT
TCGCAATCGGCCAAACGCGGGGTGCTGGTGGCGATGAACGACAGCGTGCTGGACGGACGTGATGTGACAAAAACCAACAC
CACATCGGTACAGACTTTCCAGTCGCCTAACTTTGGCCCGCTTGGTTATATCCATAATGGCAAGGTTGACTATCAGCGTT
CACCGCAGCGCAAGCACACCACGGATACGCCGTTTGACGTCTCCGGTCTGACTGAGTTGCCCAAAGTCGGCATTATCTAT
AATTATGCCAACGCCTCCGATGCGCCTGCCAAGGCGTTGATTGCCGAAGGTTATCAGGGCATCGTCAGTGCCGGTGTCGG
AAACGGTAACCTGTACAAAACGGTGTTTGACACGCTGGCGACGGCCGCCCATAACGGCGTCGCCGTGGTGCGTTCTTCCA
GAGTGCCCACCGGGGCGACTACCGAGGATGCCGAAGTGGATGACGCCAAATTTGGTTTTGTTGCCGCAGGCACGTTAAAC
CCGCAGAAAGCGCGGATCCTGCTGCAATTGGCGCTGACGCAGACCAAAGACGCCAAACAAATTCAGCAGATGTTTAATCA
ATACTGA

Upstream 100 bases:

>100_bases
TACGCCCAAGCTCCACGTATTCCTAAATCGGTGGCAAAGTCACAGTTTTTTTGAGGATAAGGGCCATAATTCAGCGGGTT
ATCTATCATGGAGAAACCTA

Downstream 100 bases:

>100_bases
GTCCCTTTTCTTCATTCGGGGGCCTGCGCGGCCCCCAGCCATGTCGGCTGAATACAGCATAAAAAACCGCGTTAACCCAC
TGAATTGGCCGCTGTCACAG

Product: L-asparaginase II

Products: NA

Alternate protein names: L-asparaginase II; L-ASNase II; L-asparagine amidohydrolase II; Colaspase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKSVKLSVVALLLAGASGSALALPTITLLATGGTIAGGGESATKSNYTAGKLGVDALVNAVPELKNVANIQGEQVVNIGS
QDMNDEVWLKLAKKINADCAKTDGFVITHGTDTLEETAYFLDLTVKCDKPVVIVGAMRPATAMSADGPFNLYNAVVTAAD
SQSAKRGVLVAMNDSVLDGRDVTKTNTTSVQTFQSPNFGPLGYIHNGKVDYQRSPQRKHTTDTPFDVSGLTELPKVGIIY
NYANASDAPAKALIAEGYQGIVSAGVGNGNLYKTVFDTLATAAHNGVAVVRSSRVPTGATTEDAEVDDAKFGFVAAGTLN
PQKARILLQLALTQTKDAKQIQQMFNQY

Sequences:

>Translated_348_residues
MKSVKLSVVALLLAGASGSALALPTITLLATGGTIAGGGESATKSNYTAGKLGVDALVNAVPELKNVANIQGEQVVNIGS
QDMNDEVWLKLAKKINADCAKTDGFVITHGTDTLEETAYFLDLTVKCDKPVVIVGAMRPATAMSADGPFNLYNAVVTAAD
SQSAKRGVLVAMNDSVLDGRDVTKTNTTSVQTFQSPNFGPLGYIHNGKVDYQRSPQRKHTTDTPFDVSGLTELPKVGIIY
NYANASDAPAKALIAEGYQGIVSAGVGNGNLYKTVFDTLATAAHNGVAVVRSSRVPTGATTEDAEVDDAKFGFVAAGTLN
PQKARILLQLALTQTKDAKQIQQMFNQY
>Mature_348_residues
MKSVKLSVVALLLAGASGSALALPTITLLATGGTIAGGGESATKSNYTAGKLGVDALVNAVPELKNVANIQGEQVVNIGS
QDMNDEVWLKLAKKINADCAKTDGFVITHGTDTLEETAYFLDLTVKCDKPVVIVGAMRPATAMSADGPFNLYNAVVTAAD
SQSAKRGVLVAMNDSVLDGRDVTKTNTTSVQTFQSPNFGPLGYIHNGKVDYQRSPQRKHTTDTPFDVSGLTELPKVGIIY
NYANASDAPAKALIAEGYQGIVSAGVGNGNLYKTVFDTLATAAHNGVAVVRSSRVPTGATTEDAEVDDAKFGFVAAGTLN
PQKARILLQLALTQTKDAKQIQQMFNQY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=227, Percent_Identity=28.1938325991189, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789327, Length=348, Percent_Identity=81.3218390804598, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1788065, Length=281, Percent_Identity=28.1138790035587, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI193207372, Length=227, Percent_Identity=28.6343612334802, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558278, Length=233, Percent_Identity=28.755364806867, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=233, Percent_Identity=28.755364806867, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193207374, Length=233, Percent_Identity=28.755364806867, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323189, Length=365, Percent_Identity=40.8219178082192, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6323187, Length=365, Percent_Identity=40.8219178082192, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6323186, Length=365, Percent_Identity=40.8219178082192, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6323184, Length=365, Percent_Identity=40.8219178082192, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6320527, Length=350, Percent_Identity=37.7142857142857, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24645530, Length=218, Percent_Identity=30.7339449541284, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24639609, Length=225, Percent_Identity=29.3333333333333, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36473; Mature: 36473

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVKLSVVALLLAGASGSALALPTITLLATGGTIAGGGESATKSNYTAGKLGVDALVNA
CCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHH
VPELKNVANIQGEQVVNIGSQDMNDEVWLKLAKKINADCAKTDGFVITHGTDTLEETAYF
HHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCEEE
LDLTVKCDKPVVIVGAMRPATAMSADGPFNLYNAVVTAADSQSAKRGVLVAMNDSVLDGR
EEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCEEEEECCCCCCCC
DVTKTNTTSVQTFQSPNFGPLGYIHNGKVDYQRSPQRKHTTDTPFDVSGLTELPKVGIIY
CCCCCCCCEEEEECCCCCCCCCEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NYANASDAPAKALIAEGYQGIVSAGVGNGNLYKTVFDTLATAAHNGVAVVRSSRVPTGAT
EECCCCCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
TEDAEVDDAKFGFVAAGTLNPQKARILLQLALTQTKDAKQIQQMFNQY
CCCCCCCCCCCCEEEECCCCHHHHHEEEEEHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKSVKLSVVALLLAGASGSALALPTITLLATGGTIAGGGESATKSNYTAGKLGVDALVNA
CCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHH
VPELKNVANIQGEQVVNIGSQDMNDEVWLKLAKKINADCAKTDGFVITHGTDTLEETAYF
HHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCEEE
LDLTVKCDKPVVIVGAMRPATAMSADGPFNLYNAVVTAADSQSAKRGVLVAMNDSVLDGR
EEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCEEEEECCCCCCCC
DVTKTNTTSVQTFQSPNFGPLGYIHNGKVDYQRSPQRKHTTDTPFDVSGLTELPKVGIIY
CCCCCCCCEEEEECCCCCCCCCEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NYANASDAPAKALIAEGYQGIVSAGVGNGNLYKTVFDTLATAAHNGVAVVRSSRVPTGAT
EECCCCCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
TEDAEVDDAKFGFVAAGTLNPQKARILLQLALTQTKDAKQIQQMFNQY
CCCCCCCCCCCCEEEECCCCHHHHHEEEEEHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2407723; 2144836; 9278503; 6766894; 387570; 321449; 4561256; 1906013; 1521538; 1287659; 8434007; 8706862 [H]