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The map label for this gene is poxB [H]

Identifier: 157369907

GI number: 157369907

Start: 1817861

End: 1819582

Strand: Reverse

Name: poxB [H]

Synonym: Spro_1664

Alternate gene names: 157369907

Gene position: 1819582-1817861 (Counterclockwise)

Preceding gene: 157369908

Following gene: 157369906

Centisome position: 33.39

GC content: 59.93

Gene sequence:

>1722_bases
ATGAAGCAAACTATAGCCACACTGATCGCCAAAACTCTGGAACAGGCCGGTGTGAAACGCATTTGGGGCGTCACCGGCGA
TTCGCTCAACGGCCTCAGCGACAGCCTGCACCGTATGGGCACCATCGAATGGCTGGGAACCCGCCATGAAGAGGTCGCCG
CCTTCGCCGCCGGAGCAGAAGCCCAGTTGACGGGTCAGTTGGCGGTCTGTGCCGGCTCCTGCGGCCCCGGTAACCTGCAT
CTGATTAACGGTCTGTTCGACTGCCACCGCAATCATGTGCCGGTATTGGCGATTGCCGCACACATTCCCTCCAGCGAAAT
CGGCAGCGGCTATTTCCAGGAAACCCACCCGCAAGAACTATTTCGCGAATGCAGCCACTACTGTGAACTGGTCTCTAACC
CGGAACAGTTGCCGCGGGTGCTGGAAATCGCCATGCGCAAGGCGATCCTCAATCGCGGCGTGTCGGTGATCGTCCTGCCG
GGCGACGTGGCGCTGCGGATGGCACCGGAAGACGCCAGCATGGTGTGGCACACACCGGCACTGCCGCTGGTGCAACCGCC
AATGAGCGAACTGAACAGGCTGGCGGAGACCCTGAACAAGGCGAAGAACATTACGCTGATGTGCGGCAGCGGTTGCGCCG
GCTCCCATGACGAAGTGGTCAAGTTGGCCGAACTGCTGCAGGCTCCGGTGGTTCATGCACTGCGCGGTAAAGAGCATATC
GAATGGGACAACCCCTACAGCGTCGGCATGACCGGGCTGATCGGTTTCTCTTCCGGCTACCACGCCATGATGAACGCCGA
CACACTGGTATTGCTCGGTACCCAGTTCCCGTACCGTGCCTTCTATCCGACCAATGCCAATATCATTCAGATCGACATCA
ACCCCGGCAGCATCGGCGCACACTGCCCGGTCAATATGGCGCTGGTGGGGGATATCCATACCACCCTCACTGCCCTGCTG
CCGCAGTTGGAACCCAAAAGCGACCGTGCGTTCCTCGACAAGGCGCTGGAGCACTACCGCACTGCGCGCAAGGATCTCGA
CGGGCTGGCGACGGCCAATGACGATCAGCCGATTCACCCGCAGTATCTGGCGCAACAGCTCAGCCAATACGCCAGCGATG
ACGCCATCTTCACCTGCGACGTCGGTACGCCAACGGTGTGGGCCGCTCGTTATCTGAAGATGAACGGCAAACGCCGCCTG
CTCGGGTCGTTCAATCATGGCTCGATGGCGAACGCCATGCCACAGGCGATTGGCGCACAGGCCACCGAACCGGACAAACA
GGTGATCGCCATGTGCGGCGACGGCGGCTTCACCATGCTGATGGGGGATTTCCTGTCGCTGGCCCAACTGAAACTGCCGG
TCAAGATTGTAATTTTCAACAACAGCGTGCTGGGCTTCGTGGCGATGGAAATGAAGGCCGGTGGCTACCTGACTGACGGC
ACCGATCTGCACAATCCTGATTTTGCGGCGATTGCCAACGCCGCCGGCATCAAAGGCATTCGCGTGGAGAAAGCTTCTGA
TTTGGATGGGGCGCTGCAGGAAATGCTGGCGCATCCCGGCCCGGCACTGCTGGACGTCGTCACCGCCAAACAGGAACTGG
CGATGCCGCCGCAGATCAAATTCGAGCAGGCCAAGGGCTTTAGCCTGTATATGCTGCGCGCCATTATCAACGGCCGCGGT
GACGAAGTGGTCGAGCTGGCAAAAACCAACTGGTTGCGTTAA

Upstream 100 bases:

>100_bases
AAAGACTGATAAGTCATACCCAATAGATTTCAAGCCACGGCCAGGCGGCACACGCAGTGGTTTGAAAGATAAAGGGACCT
TTACAACGCTTGGGGGAATT

Downstream 100 bases:

>100_bases
TAATCGTGTCCTCTTTTAGCTAAAACTATCTCCAACAAATTTCAAGTTGCAGCCAACAACGCTGCGGCTTGAAAGATGAA
GGGGAAAAGGATTTTTATGC

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKQTIATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAEAQLTGQLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSNPEQLPRVLEIAMRKAILNRGVSVIVLP
GDVALRMAPEDASMVWHTPALPLVQPPMSELNRLAETLNKAKNITLMCGSGCAGSHDEVVKLAELLQAPVVHALRGKEHI
EWDNPYSVGMTGLIGFSSGYHAMMNADTLVLLGTQFPYRAFYPTNANIIQIDINPGSIGAHCPVNMALVGDIHTTLTALL
PQLEPKSDRAFLDKALEHYRTARKDLDGLATANDDQPIHPQYLAQQLSQYASDDAIFTCDVGTPTVWAARYLKMNGKRRL
LGSFNHGSMANAMPQAIGAQATEPDKQVIAMCGDGGFTMLMGDFLSLAQLKLPVKIVIFNNSVLGFVAMEMKAGGYLTDG
TDLHNPDFAAIANAAGIKGIRVEKASDLDGALQEMLAHPGPALLDVVTAKQELAMPPQIKFEQAKGFSLYMLRAIINGRG
DEVVELAKTNWLR

Sequences:

>Translated_573_residues
MKQTIATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAEAQLTGQLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSNPEQLPRVLEIAMRKAILNRGVSVIVLP
GDVALRMAPEDASMVWHTPALPLVQPPMSELNRLAETLNKAKNITLMCGSGCAGSHDEVVKLAELLQAPVVHALRGKEHI
EWDNPYSVGMTGLIGFSSGYHAMMNADTLVLLGTQFPYRAFYPTNANIIQIDINPGSIGAHCPVNMALVGDIHTTLTALL
PQLEPKSDRAFLDKALEHYRTARKDLDGLATANDDQPIHPQYLAQQLSQYASDDAIFTCDVGTPTVWAARYLKMNGKRRL
LGSFNHGSMANAMPQAIGAQATEPDKQVIAMCGDGGFTMLMGDFLSLAQLKLPVKIVIFNNSVLGFVAMEMKAGGYLTDG
TDLHNPDFAAIANAAGIKGIRVEKASDLDGALQEMLAHPGPALLDVVTAKQELAMPPQIKFEQAKGFSLYMLRAIINGRG
DEVVELAKTNWLR
>Mature_573_residues
MKQTIATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAEAQLTGQLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSNPEQLPRVLEIAMRKAILNRGVSVIVLP
GDVALRMAPEDASMVWHTPALPLVQPPMSELNRLAETLNKAKNITLMCGSGCAGSHDEVVKLAELLQAPVVHALRGKEHI
EWDNPYSVGMTGLIGFSSGYHAMMNADTLVLLGTQFPYRAFYPTNANIIQIDINPGSIGAHCPVNMALVGDIHTTLTALL
PQLEPKSDRAFLDKALEHYRTARKDLDGLATANDDQPIHPQYLAQQLSQYASDDAIFTCDVGTPTVWAARYLKMNGKRRL
LGSFNHGSMANAMPQAIGAQATEPDKQVIAMCGDGGFTMLMGDFLSLAQLKLPVKIVIFNNSVLGFVAMEMKAGGYLTDG
TDLHNPDFAAIANAAGIKGIRVEKASDLDGALQEMLAHPGPALLDVVTAKQELAMPPQIKFEQAKGFSLYMLRAIINGRG
DEVVELAKTNWLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=24.6428571428571, Blast_Score=133, Evalue=6e-31,
Organism=Escherichia coli, GI1787096, Length=573, Percent_Identity=79.5811518324607, Blast_Score=937, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=28.9285714285714, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=28.8566243194192, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786717, Length=549, Percent_Identity=25.3187613843352, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=24.5487364620939, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17531299, Length=491, Percent_Identity=27.6985743380855, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=491, Percent_Identity=27.6985743380855, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=27.1276595744681, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6320816, Length=542, Percent_Identity=22.6937269372694, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=492, Percent_Identity=21.3414634146341, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI19922626, Length=478, Percent_Identity=27.6150627615063, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 61906; Mature: 61906

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTIATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAE
CHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHCCCC
AQLTGQLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEEECEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSNPEQLPRVLEIAMRKAILNRGVSVIVLPGDVALRMAPEDASMVWHTPA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCEEEECCC
LPLVQPPMSELNRLAETLNKAKNITLMCGSGCAGSHDEVVKLAELLQAPVVHALRGKEHI
CCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
EWDNPYSVGMTGLIGFSSGYHAMMNADTLVLLGTQFPYRAFYPTNANIIQIDINPGSIGA
CCCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCC
HCPVNMALVGDIHTTLTALLPQLEPKSDRAFLDKALEHYRTARKDLDGLATANDDQPIHP
CCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
QYLAQQLSQYASDDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQAIGAQ
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCC
ATEPDKQVIAMCGDGGFTMLMGDFLSLAQLKLPVKIVIFNNSVLGFVAMEMKAGGYLTDG
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCEECCC
TDLHNPDFAAIANAAGIKGIRVEKASDLDGALQEMLAHPGPALLDVVTAKQELAMPPQIK
CCCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
FEQAKGFSLYMLRAIINGRGDEVVELAKTNWLR
HHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKQTIATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAE
CHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHCCCC
AQLTGQLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEEECEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSNPEQLPRVLEIAMRKAILNRGVSVIVLPGDVALRMAPEDASMVWHTPA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCEEEECCC
LPLVQPPMSELNRLAETLNKAKNITLMCGSGCAGSHDEVVKLAELLQAPVVHALRGKEHI
CCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
EWDNPYSVGMTGLIGFSSGYHAMMNADTLVLLGTQFPYRAFYPTNANIIQIDINPGSIGA
CCCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCC
HCPVNMALVGDIHTTLTALLPQLEPKSDRAFLDKALEHYRTARKDLDGLATANDDQPIHP
CCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
QYLAQQLSQYASDDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQAIGAQ
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCC
ATEPDKQVIAMCGDGGFTMLMGDFLSLAQLKLPVKIVIFNNSVLGFVAMEMKAGGYLTDG
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCEECCC
TDLHNPDFAAIANAAGIKGIRVEKASDLDGALQEMLAHPGPALLDVVTAKQELAMPPQIK
CCCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
FEQAKGFSLYMLRAIINGRGDEVVELAKTNWLR
HHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]