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The map label for this gene is amsH [H]

Identifier: 157369826

GI number: 157369826

Start: 1727572

End: 1728708

Strand: Direct

Name: amsH [H]

Synonym: Spro_1583

Alternate gene names: 157369826

Gene position: 1727572-1728708 (Clockwise)

Preceding gene: 157369825

Following gene: 157369827

Centisome position: 31.71

GC content: 42.48

Gene sequence:

>1137_bases
ATGGCAAAAAAACAATGGAAACTGATACCGATTTTGGTATCGATTAGTGTGATCAGTGGTTGTACAATCATCCCCGGTAC
CCACCTTTCCACTAGTGGTAAAGATATTGTAAAACAAGAAGATAGCGATTTTGATATTGATAAACTGGTAAACGTCTATC
CAATGACACCAGGATTGATTAGCAAAATGAGGCCAAAGCCTGCGATAGCGCAACCGAATAGTGCATTGGAACGTGAATTG
CAGAATTATGAGTATCGCATTGGTGTAGGTGACGTTTTAATGGTGACCGTTTGGGATCATCCAGAATTAACTACCCCAGC
TGGTCAATACCGTAGCGCCAGCGATACTGGTAACTGGGTGAATTCTGATGGAACCATATTCTATCCTTATATCGGCAAGG
TAAAAGTTGTTGGTAAAACTGTTGTCCAAGTTAGAAATGAAATCTCATCGCGTTTATCTCACTATATAGAAAGTCCTCAG
GTTGATGTGAGTATTGCAGCATTCCGGTCACAGAAAACGTATATCACAGGAGAGGTTTCTAAATCCGGTCAGCAAGCTAT
TACCAACGTGCCGTTAACTGTGATGGATGCTATCAACGCCGCAGGCGGTTTGGCAGAAAATGCTGATTGGCGTAATGTTG
TATTAACTCATAATGGTAAAGAGCAGCGTATTTCTCTACAGGCGTTAATGCAAAAAGGCGATTTGACACAAAACCATCTT
CTTTATCCAGGCGATATTCTTTACGTACCACGTAATGATGATTTGAAAGTGTTTGTCATGGGTGAAGTTAAAACTCCTGC
GACACTAAAAATGGATAGAAGCGGGATGACTTTGACTGAAGCTTTGGGAAATTCGGAAGGCATCAGCCAATCCACCGCAG
ATGCGACTGGTGTTTTTGTTATTCGCCCATTACATGGTACTGAAGGTAACAAGCTTGCCAATATTTACCAATTAAACATG
GCAGATGCGACTGCGATGGTTATGGGGGCTGAGTTCCATTTGCAGCCATATGATATTGTGTATGTGACGGCAGCTCCCGT
TGCCCGCTGGAATCGTTTGATCAGTCAGTTAGCTCCGACTATTTCTAGCTATAATGATCTCAGTGAAGGTACGTTACGAA
TTCGTAATTGGCCGTAG

Upstream 100 bases:

>100_bases
GAGCAGATGACAAATCATTTTGCTGAGTGCCGTGAAAGCGCTATGGTGACCCGGGGCTTAGCACCGCAAACATTCTTACT
TTGATAGTGATGATAACTCG

Downstream 100 bases:

>100_bases
GAATAAATAATGTTTGATTCAATACTGGTAGTTTGCGTTGGGAATATTTGCCGTTCTCCGATAGCAGAAAGGCTACTACG
CAAAGCGTTGCCAAATAAGA

Product: polysaccharide export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MAKKQWKLIPILVSISVISGCTIIPGTHLSTSGKDIVKQEDSDFDIDKLVNVYPMTPGLISKMRPKPAIAQPNSALEREL
QNYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVKVVGKTVVQVRNEISSRLSHYIESPQ
VDVSIAAFRSQKTYITGEVSKSGQQAITNVPLTVMDAINAAGGLAENADWRNVVLTHNGKEQRISLQALMQKGDLTQNHL
LYPGDILYVPRNDDLKVFVMGEVKTPATLKMDRSGMTLTEALGNSEGISQSTADATGVFVIRPLHGTEGNKLANIYQLNM
ADATAMVMGAEFHLQPYDIVYVTAAPVARWNRLISQLAPTISSYNDLSEGTLRIRNWP

Sequences:

>Translated_378_residues
MAKKQWKLIPILVSISVISGCTIIPGTHLSTSGKDIVKQEDSDFDIDKLVNVYPMTPGLISKMRPKPAIAQPNSALEREL
QNYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVKVVGKTVVQVRNEISSRLSHYIESPQ
VDVSIAAFRSQKTYITGEVSKSGQQAITNVPLTVMDAINAAGGLAENADWRNVVLTHNGKEQRISLQALMQKGDLTQNHL
LYPGDILYVPRNDDLKVFVMGEVKTPATLKMDRSGMTLTEALGNSEGISQSTADATGVFVIRPLHGTEGNKLANIYQLNM
ADATAMVMGAEFHLQPYDIVYVTAAPVARWNRLISQLAPTISSYNDLSEGTLRIRNWP
>Mature_377_residues
AKKQWKLIPILVSISVISGCTIIPGTHLSTSGKDIVKQEDSDFDIDKLVNVYPMTPGLISKMRPKPAIAQPNSALERELQ
NYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVKVVGKTVVQVRNEISSRLSHYIESPQV
DVSIAAFRSQKTYITGEVSKSGQQAITNVPLTVMDAINAAGGLAENADWRNVVLTHNGKEQRISLQALMQKGDLTQNHLL
YPGDILYVPRNDDLKVFVMGEVKTPATLKMDRSGMTLTEALGNSEGISQSTADATGVFVIRPLHGTEGNKLANIYQLNMA
DATAMVMGAEFHLQPYDIVYVTAAPVARWNRLISQLAPTISSYNDLSEGTLRIRNWP

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. Might be involved in the translocation of polysaccharides across the outer membrane [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1788376, Length=379, Percent_Identity=69.9208443271768, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1787218, Length=378, Percent_Identity=66.4021164021164, Blast_Score=551, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 41535; Mature: 41404

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKQWKLIPILVSISVISGCTIIPGTHLSTSGKDIVKQEDSDFDIDKLVNVYPMTPGLI
CCCCCEEEEEEEEEEHHHCCCEECCCCCCCCCCCHHHHCCCCCCCHHHHHEEECCCCHHH
SKMRPKPAIAQPNSALERELQNYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWV
HHCCCCCCCCCCCHHHHHHHHCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCEE
NSDGTIFYPYIGKVKVVGKTVVQVRNEISSRLSHYIESPQVDVSIAAFRSQKTYITGEVS
CCCCCEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECC
KSGQQAITNVPLTVMDAINAAGGLAENADWRNVVLTHNGKEQRISLQALMQKGDLTQNHL
CCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCE
LYPGDILYVPRNDDLKVFVMGEVKTPATLKMDRSGMTLTEALGNSEGISQSTADATGVFV
ECCCCEEEEECCCCEEEEEEECCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCEEEE
IRPLHGTEGNKLANIYQLNMADATAMVMGAEFHLQPYDIVYVTAAPVARWNRLISQLAPT
EEECCCCCCCEEEEEEEEECCCCEEEEECCEEECCCEEEEEEECCCHHHHHHHHHHHHHH
ISSYNDLSEGTLRIRNWP
HHCCCCCCCCEEEEECCC
>Mature Secondary Structure 
AKKQWKLIPILVSISVISGCTIIPGTHLSTSGKDIVKQEDSDFDIDKLVNVYPMTPGLI
CCCCEEEEEEEEEEHHHCCCEECCCCCCCCCCCHHHHCCCCCCCHHHHHEEECCCCHHH
SKMRPKPAIAQPNSALERELQNYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWV
HHCCCCCCCCCCCHHHHHHHHCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCEE
NSDGTIFYPYIGKVKVVGKTVVQVRNEISSRLSHYIESPQVDVSIAAFRSQKTYITGEVS
CCCCCEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECC
KSGQQAITNVPLTVMDAINAAGGLAENADWRNVVLTHNGKEQRISLQALMQKGDLTQNHL
CCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCE
LYPGDILYVPRNDDLKVFVMGEVKTPATLKMDRSGMTLTEALGNSEGISQSTADATGVFV
ECCCCEEEEECCCCEEEEEEECCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCEEEE
IRPLHGTEGNKLANIYQLNMADATAMVMGAEFHLQPYDIVYVTAAPVARWNRLISQLAPT
EEECCCCCCCEEEEEEEEECCCCEEEEECCEEECCCEEEEEEECCCHHHHHHHHHHHHHH
ISSYNDLSEGTLRIRNWP
HHCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]