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The map label for this gene is rlmF

Identifier: 157369713

GI number: 157369713

Start: 1602761

End: 1603771

Strand: Direct

Name: rlmF

Synonym: Spro_1470

Alternate gene names: 157369713

Gene position: 1602761-1603771 (Clockwise)

Preceding gene: 157369711

Following gene: 157369714

Centisome position: 29.41

GC content: 57.37

Gene sequence:

>1011_bases
ATGGTATCTTACGTGCCAGAAATACCTTTAAGAGAGCCCGTCACGGTGGAAAAAAAGAAAAGCTTCCCGCAGCAGAAAAG
CGGACTGCACCCGCGTAACCGTCATCGCTCACGCTATGACTTTCCGGCGCTGATCGCCAGCTGCCCGGCGTTGGAGCCGT
TCGTCAAGCCGAACGCCTGGGGCGATATCTCGGTAGATTTTGCCGATCCGGCGGCGGTTAAAATGCTCAACCGTGCGTTG
CTGCAACACTTCTATGGTATCGAGCACTGGGATATTCCCGCCGATTACCTGTGCCCGCCAATCCCGGGGCGCGCCGATTA
CCTGCACCATCTGGCCGATCTGCTGGCGACCAGCAACGACGGTGAGATCCCGCGTGGCAAAGGCGTGGCGATCCTTGACG
TGGGCATTGGTGCCAACTGCATTTATCCGATCGTAGGCCTGCGCGAATACGGCTGGCGTTTTACCGGTTCAGAGATTGAC
CCGGTGTCGCTCAATTCGGCCAAAATGATCGTCGAAATGAACCCGACGTTAAGAAACAGCGTGCGTCTGCGCCTGCAAAA
ACAGCCGGAGTTTATCTTTAACGGCATCATTGGTGTGGCTGAGAAGTTTGACGCTACGCTGTGCAACCCGCCATTCCACG
GCTCCGAGCAGGAAGCGCAGGCCAGCACCCGCCGCAAACTGCACAAGTTGGGCAAGGGCGAAGTGGCCGACAAGCCGGTA
CAGAACTTTGGCGGCAAGAATAACGAACTGTGGTGTGAAGGCGGCGAAGAAGCCTTCGTGCGTAAAATGGTCGAAGAAAG
CGTCAGCAAGGCGCAGAACTGCCTGTGGTTCACTTCTTTGATATCCAAACACACCACCTTGCCGTCGATTTATCACGCGG
CGAAGTTGGCCGGGGTGGCGGAAGTGCGCACTATCGAGATGGCGCAGGGGCAAAAAATCAGCCGCTTTGTCGCCTGGACC
TTCCATGACGCCGAGCAACAGGCCGCCTGGGCGGCAGAACGCTGGCGCTAA

Upstream 100 bases:

>100_bases
AGTAATAACGGGACTCACTGCTCATAGGTGGCCTGAGATAGGGGAAACAGAGCTAATATTTTGACGTGTTTCCCGCCGCC
TGCAAGTTTGTCTTCGCTTT

Downstream 100 bases:

>100_bases
TTTCGGAGTTTGCACAGATTTTTGCCGGTATGGGAGGCAGCTCCCATACCGACTTTAGGCTTGACACCCTTTCTTGACAT
ATCCCATTAAGGTTCCTTAA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MVSYVPEIPLREPVTVEKKKSFPQQKSGLHPRNRHRSRYDFPALIASCPALEPFVKPNAWGDISVDFADPAAVKMLNRAL
LQHFYGIEHWDIPADYLCPPIPGRADYLHHLADLLATSNDGEIPRGKGVAILDVGIGANCIYPIVGLREYGWRFTGSEID
PVSLNSAKMIVEMNPTLRNSVRLRLQKQPEFIFNGIIGVAEKFDATLCNPPFHGSEQEAQASTRRKLHKLGKGEVADKPV
QNFGGKNNELWCEGGEEAFVRKMVEESVSKAQNCLWFTSLISKHTTLPSIYHAAKLAGVAEVRTIEMAQGQKISRFVAWT
FHDAEQQAAWAAERWR

Sequences:

>Translated_336_residues
MVSYVPEIPLREPVTVEKKKSFPQQKSGLHPRNRHRSRYDFPALIASCPALEPFVKPNAWGDISVDFADPAAVKMLNRAL
LQHFYGIEHWDIPADYLCPPIPGRADYLHHLADLLATSNDGEIPRGKGVAILDVGIGANCIYPIVGLREYGWRFTGSEID
PVSLNSAKMIVEMNPTLRNSVRLRLQKQPEFIFNGIIGVAEKFDATLCNPPFHGSEQEAQASTRRKLHKLGKGEVADKPV
QNFGGKNNELWCEGGEEAFVRKMVEESVSKAQNCLWFTSLISKHTTLPSIYHAAKLAGVAEVRTIEMAQGQKISRFVAWT
FHDAEQQAAWAAERWR
>Mature_336_residues
MVSYVPEIPLREPVTVEKKKSFPQQKSGLHPRNRHRSRYDFPALIASCPALEPFVKPNAWGDISVDFADPAAVKMLNRAL
LQHFYGIEHWDIPADYLCPPIPGRADYLHHLADLLATSNDGEIPRGKGVAILDVGIGANCIYPIVGLREYGWRFTGSEID
PVSLNSAKMIVEMNPTLRNSVRLRLQKQPEFIFNGIIGVAEKFDATLCNPPFHGSEQEAQASTRRKLHKLGKGEVADKPV
QNFGGKNNELWCEGGEEAFVRKMVEESVSKAQNCLWFTSLISKHTTLPSIYHAAKLAGVAEVRTIEMAQGQKISRFVAWT
FHDAEQQAAWAAERWR

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=298, Percent_Identity=30.2013422818792, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI87081786, Length=303, Percent_Identity=54.7854785478548, Blast_Score=343, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI32564931, Length=317, Percent_Identity=24.9211356466877, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI32564933, Length=317, Percent_Identity=23.0283911671924, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281363415, Length=304, Percent_Identity=27.9605263157895, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI19922302, Length=304, Percent_Identity=27.9605263157895, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_SERP5 (A8GBT4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477702.1
- ProteinModelPortal:   A8GBT4
- SMR:   A8GBT4
- STRING:   A8GBT4
- GeneID:   5607306
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1470
- eggNOG:   COG3129
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- ProtClustDB:   PRK11727
- BioCyc:   SPRO399741:SPRO_1470-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 37640; Mature: 37640

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSYVPEIPLREPVTVEKKKSFPQQKSGLHPRNRHRSRYDFPALIASCPALEPFVKPNAW
CCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCCHHCCCCCCHHHHHHCCCCCCCCCCCCCC
GDISVDFADPAAVKMLNRALLQHFYGIEHWDIPADYLCPPIPGRADYLHHLADLLATSND
CCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCC
GEIPRGKGVAILDVGIGANCIYPIVGLREYGWRFTGSEIDPVSLNSAKMIVEMNPTLRNS
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCEECCCCEEEEEECCCHHHH
VRLRLQKQPEFIFNGIIGVAEKFDATLCNPPFHGSEQEAQASTRRKLHKLGKGEVADKPV
HHEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCH
QNFGGKNNELWCEGGEEAFVRKMVEESVSKAQNCLWFTSLISKHTTLPSIYHAAKLAGVA
HHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EVRTIEMAQGQKISRFVAWTFHDAEQQAAWAAERWR
HHHHHHHHCCCCHHHEEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MVSYVPEIPLREPVTVEKKKSFPQQKSGLHPRNRHRSRYDFPALIASCPALEPFVKPNAW
CCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCCHHCCCCCCHHHHHHCCCCCCCCCCCCCC
GDISVDFADPAAVKMLNRALLQHFYGIEHWDIPADYLCPPIPGRADYLHHLADLLATSND
CCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCC
GEIPRGKGVAILDVGIGANCIYPIVGLREYGWRFTGSEIDPVSLNSAKMIVEMNPTLRNS
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCEECCCCEEEEEECCCHHHH
VRLRLQKQPEFIFNGIIGVAEKFDATLCNPPFHGSEQEAQASTRRKLHKLGKGEVADKPV
HHEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCH
QNFGGKNNELWCEGGEEAFVRKMVEESVSKAQNCLWFTSLISKHTTLPSIYHAAKLAGVA
HHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EVRTIEMAQGQKISRFVAWTFHDAEQQAAWAAERWR
HHHHHHHHCCCCHHHEEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA