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The map label for this gene is map [H]

Identifier: 157369608

GI number: 157369608

Start: 1494669

End: 1495463

Strand: Reverse

Name: map [H]

Synonym: Spro_1365

Alternate gene names: 157369608

Gene position: 1495463-1494669 (Counterclockwise)

Preceding gene: 157369609

Following gene: 157369607

Centisome position: 27.45

GC content: 55.85

Gene sequence:

>795_bases
GTGAAAGAGATTGTGATCAAAACGCCGGAACAGATTGCCAAAATGCGTCACTCTGGCGCCCTGCTGGCGCAGGTGTTTGC
TATGCTCGATCAGGTGATTGTCGAGGGCATCTCCACTATGGAGATCAACGACCGCGCCGAGGCCTTTATTGTCAATGAGC
TGAAATCGCGCCCGGCCAGCAAAGGGCAGTACGATTTTCCTTATGTGCTCAATACGTCGATCGACGACGTTATCTGCCAC
GGCATTCCGTCGGTCAGCAAAATCTTGCGTTCCGGCATGATTGTTAACGTCGATATTACGCTGGAAAACGACGGCTACAT
TGCCGACTCCAGCAAGATGTACTGCATCGGCCAGACCACGCCGGTCGCCAAACGCCTGGTGAACAACGTGTATGAGTCGT
TGTGGCAAGGCATCCGGGCAGTAAAACCGGGGGCGACGCTGGGTGATATCGGCCATGCAATTCAGCAGCACGCCGAACAG
GCCGGCTACAGTATTGTGCGTGAGTACTGCGGTCACGGTATCGGCCGGGATATGCACGAAGAGCCGGCGGTGCTGCACTT
TGGCCAACCGGGAACCGGTATGGTGTTGCAGGAAGGCATGGTGTTTACCATCGAACCGATGATTAACCAGGGCGACCACC
GGATTAAACAGAAGAAAGACGGTTGGACGGTGGTAACGCGCGACAAGAAACTTTCGGCCCAATGGGAACACACCGTGGCA
GTCACCGCCGACGGCGTGGAGATCCTGACGCTGCGGGACGAAGAACGCCAGGCCGGTTTTGCAGAACGTTACTAA

Upstream 100 bases:

>100_bases
AGATTGGCATGTTGGCGGAGTTCCATCCGGAACTGACCTATCCCCAACTGGTAAAAAAAATGATGAAAGATAACGCGCTG
ACGTTGAAGGAGATTGTCGG

Downstream 100 bases:

>100_bases
GCCACCAGGCTAGCAACCTAATAAGTTAGTAATTCAATTCATACCCTATAGCTTTCAAGTTGTAGCTAGGCGGCCAACTC
GCTCATCCCCAGGCGCTTAC

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKEIVIKTPEQIAKMRHSGALLAQVFAMLDQVIVEGISTMEINDRAEAFIVNELKSRPASKGQYDFPYVLNTSIDDVICH
GIPSVSKILRSGMIVNVDITLENDGYIADSSKMYCIGQTTPVAKRLVNNVYESLWQGIRAVKPGATLGDIGHAIQQHAEQ
AGYSIVREYCGHGIGRDMHEEPAVLHFGQPGTGMVLQEGMVFTIEPMINQGDHRIKQKKDGWTVVTRDKKLSAQWEHTVA
VTADGVEILTLRDEERQAGFAERY

Sequences:

>Translated_264_residues
MKEIVIKTPEQIAKMRHSGALLAQVFAMLDQVIVEGISTMEINDRAEAFIVNELKSRPASKGQYDFPYVLNTSIDDVICH
GIPSVSKILRSGMIVNVDITLENDGYIADSSKMYCIGQTTPVAKRLVNNVYESLWQGIRAVKPGATLGDIGHAIQQHAEQ
AGYSIVREYCGHGIGRDMHEEPAVLHFGQPGTGMVLQEGMVFTIEPMINQGDHRIKQKKDGWTVVTRDKKLSAQWEHTVA
VTADGVEILTLRDEERQAGFAERY
>Mature_264_residues
MKEIVIKTPEQIAKMRHSGALLAQVFAMLDQVIVEGISTMEINDRAEAFIVNELKSRPASKGQYDFPYVLNTSIDDVICH
GIPSVSKILRSGMIVNVDITLENDGYIADSSKMYCIGQTTPVAKRLVNNVYESLWQGIRAVKPGATLGDIGHAIQQHAEQ
AGYSIVREYCGHGIGRDMHEEPAVLHFGQPGTGMVLQEGMVFTIEPMINQGDHRIKQKKDGWTVVTRDKKLSAQWEHTVA
VTADGVEILTLRDEERQAGFAERY

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=262, Percent_Identity=41.2213740458015, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI40385867, Length=247, Percent_Identity=33.6032388663968, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1786364, Length=252, Percent_Identity=49.6031746031746, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1788728, Length=220, Percent_Identity=28.1818181818182, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71996291, Length=254, Percent_Identity=38.5826771653543, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323273, Length=269, Percent_Identity=38.6617100371747, Blast_Score=185, Evalue=6e-48,
Organism=Drosophila melanogaster, GI21355531, Length=245, Percent_Identity=37.5510204081633, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24583427, Length=251, Percent_Identity=35.4581673306773, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 29309; Mature: 29309

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIVIKTPEQIAKMRHSGALLAQVFAMLDQVIVEGISTMEINDRAEAFIVNELKSRPAS
CCCEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCC
KGQYDFPYVLNTSIDDVICHGIPSVSKILRSGMIVNVDITLENDGYIADSSKMYCIGQTT
CCCCCCCEEECCCHHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCEEECCCCEEEEECCC
PVAKRLVNNVYESLWQGIRAVKPGATLGDIGHAIQQHAEQAGYSIVREYCGHGIGRDMHE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPAVLHFGQPGTGMVLQEGMVFTIEPMINQGDHRIKQKKDGWTVVTRDKKLSAQWEHTVA
CCCEEEECCCCCCCEEECCEEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCCCEEEE
VTADGVEILTLRDEERQAGFAERY
EEECCEEEEEECCHHHHCCCCCCC
>Mature Secondary Structure
MKEIVIKTPEQIAKMRHSGALLAQVFAMLDQVIVEGISTMEINDRAEAFIVNELKSRPAS
CCCEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCC
KGQYDFPYVLNTSIDDVICHGIPSVSKILRSGMIVNVDITLENDGYIADSSKMYCIGQTT
CCCCCCCEEECCCHHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCEEECCCCEEEEECCC
PVAKRLVNNVYESLWQGIRAVKPGATLGDIGHAIQQHAEQAGYSIVREYCGHGIGRDMHE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPAVLHFGQPGTGMVLQEGMVFTIEPMINQGDHRIKQKKDGWTVVTRDKKLSAQWEHTVA
CCCEEEECCCCCCCEEECCEEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCCCEEEE
VTADGVEILTLRDEERQAGFAERY
EEECCEEEEEECCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]