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The map label for this gene is lacA [C]

Identifier: 157369605

GI number: 157369605

Start: 1491450

End: 1491920

Strand: Reverse

Name: lacA [C]

Synonym: Spro_1362

Alternate gene names: 157369605

Gene position: 1491920-1491450 (Counterclockwise)

Preceding gene: 157369607

Following gene: 157369604

Centisome position: 27.38

GC content: 54.99

Gene sequence:

>471_bases
ATGTCATCCGCCATTCGTGTTCTTCAGGCCGGTATTGTCGATGTCGATGCCGGTCAAAATGTCACCGTAGTCCAGCCCTG
CAATCTGTATGGCTGCCGACTCGGCGATGGGGTGTTCGTCGGGCCCTTTGTCGAGATCCAAAAAAACGTCAGTATTGGTG
CCCGCAGCAAAATTCAGTCACACAGTTTTATCTGTGAATATGTCACTATTGGCGCGGACTGCTTTATCGGCCACAACGTC
ACCTTTGCCAACGATCTGTTTAAAGATGGCGCGCCCAATGCCGATGCCAGCAGTTGGGGCCGCACGCAAATTGGCGATGG
TGTTTCCATCGGCTCCGGCGCCACGGTGCTGGCGGTGGAAATTTGCAGCGGTGCGGTGATCGGTGCCGGTTCGGTGGTCA
CCAAAAATATCACCCATAAAGGGATCTATGCCGGTAACCCGGCGCGTTTGCTGAGGGAGCTTGCACCATGA

Upstream 100 bases:

>100_bases
TACTAATTATAGTCTTTCGCTGAAAAAACGTTCGGAAAGCACAGACGTGGCGACGGTTTGTTTTATAATTCCCCCCTGCG
TCACTGCCGTGAGGAATGTC

Downstream 100 bases:

>100_bases
GCCAGGCGATCCAAATTGCCCCGGTCAGCCGTTTACCCGACGGCTACCTGACCAGTTGCGACTTTAGCTTTCACATCGCC
CACTACGCCCAGCCGGTTTT

Product: hexapaptide repeat-containing transferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: Hexapaptide Repeat-Containing Transferase; Transferase Hexapeptide Repeat Containing Protein; Acyltransferase; UDP-3-O-(3-Hydroxymyristoyl)-Glucosamine N-Acyltransferase; WxcM Domain-Containing Protein; Acetyltransferase Protein; Hexapeptide Repeat-Containing Acetyltransferase; Galactoside O-Acetyltransferase; CysE Serine O-Acetyltransferase; Hexapeptide Repeat Acetyltransferase; Sugar Acetyltransferase; WxcM-Like; WxcM-Like Protein; Acetyl/Acyl Transferase Related Protein; Hexapeptide Repeat-Containing Transferase; Acetyl Transferase/Isomerase; Carbonic Anhydrases/Acetyltransferases

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSSAIRVLQAGIVDVDAGQNVTVVQPCNLYGCRLGDGVFVGPFVEIQKNVSIGARSKIQSHSFICEYVTIGADCFIGHNV
TFANDLFKDGAPNADASSWGRTQIGDGVSIGSGATVLAVEICSGAVIGAGSVVTKNITHKGIYAGNPARLLRELAP

Sequences:

>Translated_156_residues
MSSAIRVLQAGIVDVDAGQNVTVVQPCNLYGCRLGDGVFVGPFVEIQKNVSIGARSKIQSHSFICEYVTIGADCFIGHNV
TFANDLFKDGAPNADASSWGRTQIGDGVSIGSGATVLAVEICSGAVIGAGSVVTKNITHKGIYAGNPARLLRELAP
>Mature_155_residues
SSAIRVLQAGIVDVDAGQNVTVVQPCNLYGCRLGDGVFVGPFVEIQKNVSIGARSKIQSHSFICEYVTIGADCFIGHNVT
FANDLFKDGAPNADASSWGRTQIGDGVSIGSGATVLAVEICSGAVIGAGSVVTKNITHKGIYAGNPARLLRELAP

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 16123; Mature: 15991

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAIRVLQAGIVDVDAGQNVTVVQPCNLYGCRLGDGVFVGPFVEIQKNVSIGARSKIQS
CCHHHHHHHCCEEEECCCCCEEEEECCCCEEEEECCCEEECCCEEEECCCCCCCCCCCCC
HSFICEYVTIGADCFIGHNVTFANDLFKDGAPNADASSWGRTQIGDGVSIGSGATVLAVE
CCEEEEEEEECCEEEECCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEE
ICSGAVIGAGSVVTKNITHKGIYAGNPARLLRELAP
ECCCEEECCCCEEECCCCCCCEECCCHHHHHHHHCC
>Mature Secondary Structure 
SSAIRVLQAGIVDVDAGQNVTVVQPCNLYGCRLGDGVFVGPFVEIQKNVSIGARSKIQS
CHHHHHHHCCEEEECCCCCEEEEECCCCEEEEECCCEEECCCEEEECCCCCCCCCCCCC
HSFICEYVTIGADCFIGHNVTFANDLFKDGAPNADASSWGRTQIGDGVSIGSGATVLAVE
CCEEEEEEEECCEEEECCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEE
ICSGAVIGAGSVVTKNITHKGIYAGNPARLLRELAP
ECCCEEECCCCEEECCCCCCCEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA