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The map label for this gene is dacA [H]

Identifier: 157369441

GI number: 157369441

Start: 1313908

End: 1315194

Strand: Reverse

Name: dacA [H]

Synonym: Spro_1198

Alternate gene names: 157369441

Gene position: 1315194-1313908 (Counterclockwise)

Preceding gene: 157369442

Following gene: 157369440

Centisome position: 24.14

GC content: 54.16

Gene sequence:

>1287_bases
ATGCCTGCCGGGATCTCATCTGTTATAGTGTGGCTCGTTTTTAATTTTACCCTCATGGATGTTGTAGTCTCGATCATGAA
ACAAGTAACTTCTTTTCGCTTAATCAAGAGCATAGCGCTCGGCACCGTTATCGCGATGAGTGCAACCTCCGTTGCGCATG
CCGATGACGTTAATATCAAGACCATGATCCCAGGGGTACCGCAAATCGATGCGGAATCCTACGTCCTGATTGATTACAAC
TCCGGTAAAGTTTTGGCGGAAATGAACGCCGATGCCCGCCGCGATCCAGCCAGTCTGACCAAAATGATGACCAGCTACGT
CATCGGCCAGGCGCTGAAAGCAGGTAAAATCGGCCAGGAAGACATGGTTACCGTGGGGCAAGACGCCTGGGCTACCGGCA
ACCCGGTATTCAAGGGTTCTTCGCTGATGTTCCTCAAGCCAGGCGACCGCGTACCGGTCTCCAAGCTGACCCGCGGCATC
AACCTGCAATCCGGTAACGACGCATGCGTGGCGATGGCCGACTACGTAGCCGGCAGCCAGGACGCGTTCGTTAACCTGAT
GAACACTTACGTCAGCAAGCTGGGCCTGCAGAATACCCACTTCCAGACGGTACACGGCCTGGATGCACAGGGTCAGTACA
GTTCGGCGCGCGATATGGCGTTGATCGGCCAGGCGCTGATCCGCGACGTGCCGGACGAATATGCGATTTATAAAGAAAAA
GAGTTCACCTTCAACAATATCCGCCAGATGAACCGTAACGGTCTGCTGTGGGATACCAGCCTGAACGTGGACGGCATCAA
AACCGGTCATACCGATGCGGCAGGCTACAACCTGGTCGCTTCCGCGACCGAAGGTCAGATGCGTCTGATCTCTGCGGTGT
TGGGCGGTCGTACTTATAAAGGCCGTGAAACCGAGAGCAAGAAACTGCTGACCTGGGGCTTCCGCTTCTTCGAAACCGTT
GCGCCACTGAAAGTGGGCAAAGAGTTTGCGTCTGAACCCGTCTGGTTCGGCGATGCGGATCGCGTTGAGTTGGGCGTAGA
CAAAGACGTTTACCTGACCATCCCACGCGGCCGGATGAAAGATCTCAAAGCCAGCTACGTGCTGAACACGCCGGAAATTC
ATGCACCACTGGCGAAAAACCAGGTTGTCGGCAGCATCAACTTCCAGCTCGATGGCAAAACCATCGATCAGCGCCCACTG
GTGGTGATGAACGAAGTGAAAGAAGGCGGTTTCTTCAGCCGTATCGTGGATTACATCAAACTGATGTTCCATCACTGGTT
CGGTTAA

Upstream 100 bases:

>100_bases
AAATATCCGGCAGGCGAACGCGGTTGATAAGCGTTCCTTTAGCTGAAACATTTTCTTTCAGCTAAAGCCAATAAAGCGCG
ATTAGTCAGAGCATTGGCTA

Downstream 100 bases:

>100_bases
CAGTTGAAATGGGCGAAATCGCCCCCATATACTGATTAACATTAACTCCCGCTCCGGCGGGAGTTATAATTTATAGAATC
GCTATACCGATAGCCGCACT

Product: D-alanyl-D-alanine carboxypeptidase fraction A

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPAGISSVIVWLVFNFTLMDVVVSIMKQVTSFRLIKSIALGTVIAMSATSVAHADDVNIKTMIPGVPQIDAESYVLIDYN
SGKVLAEMNADARRDPASLTKMMTSYVIGQALKAGKIGQEDMVTVGQDAWATGNPVFKGSSLMFLKPGDRVPVSKLTRGI
NLQSGNDACVAMADYVAGSQDAFVNLMNTYVSKLGLQNTHFQTVHGLDAQGQYSSARDMALIGQALIRDVPDEYAIYKEK
EFTFNNIRQMNRNGLLWDTSLNVDGIKTGHTDAAGYNLVASATEGQMRLISAVLGGRTYKGRETESKKLLTWGFRFFETV
APLKVGKEFASEPVWFGDADRVELGVDKDVYLTIPRGRMKDLKASYVLNTPEIHAPLAKNQVVGSINFQLDGKTIDQRPL
VVMNEVKEGGFFSRIVDYIKLMFHHWFG

Sequences:

>Translated_428_residues
MPAGISSVIVWLVFNFTLMDVVVSIMKQVTSFRLIKSIALGTVIAMSATSVAHADDVNIKTMIPGVPQIDAESYVLIDYN
SGKVLAEMNADARRDPASLTKMMTSYVIGQALKAGKIGQEDMVTVGQDAWATGNPVFKGSSLMFLKPGDRVPVSKLTRGI
NLQSGNDACVAMADYVAGSQDAFVNLMNTYVSKLGLQNTHFQTVHGLDAQGQYSSARDMALIGQALIRDVPDEYAIYKEK
EFTFNNIRQMNRNGLLWDTSLNVDGIKTGHTDAAGYNLVASATEGQMRLISAVLGGRTYKGRETESKKLLTWGFRFFETV
APLKVGKEFASEPVWFGDADRVELGVDKDVYLTIPRGRMKDLKASYVLNTPEIHAPLAKNQVVGSINFQLDGKTIDQRPL
VVMNEVKEGGFFSRIVDYIKLMFHHWFG
>Mature_427_residues
PAGISSVIVWLVFNFTLMDVVVSIMKQVTSFRLIKSIALGTVIAMSATSVAHADDVNIKTMIPGVPQIDAESYVLIDYNS
GKVLAEMNADARRDPASLTKMMTSYVIGQALKAGKIGQEDMVTVGQDAWATGNPVFKGSSLMFLKPGDRVPVSKLTRGIN
LQSGNDACVAMADYVAGSQDAFVNLMNTYVSKLGLQNTHFQTVHGLDAQGQYSSARDMALIGQALIRDVPDEYAIYKEKE
FTFNNIRQMNRNGLLWDTSLNVDGIKTGHTDAAGYNLVASATEGQMRLISAVLGGRTYKGRETESKKLLTWGFRFFETVA
PLKVGKEFASEPVWFGDADRVELGVDKDVYLTIPRGRMKDLKASYVLNTPEIHAPLAKNQVVGSINFQLDGKTIDQRPLV
VMNEVKEGGFFSRIVDYIKLMFHHWFG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=403, Percent_Identity=82.87841191067, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI1787062, Length=403, Percent_Identity=61.5384615384615, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI87082022, Length=362, Percent_Identity=50.828729281768, Blast_Score=393, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47076; Mature: 46945

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGISSVIVWLVFNFTLMDVVVSIMKQVTSFRLIKSIALGTVIAMSATSVAHADDVNIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TMIPGVPQIDAESYVLIDYNSGKVLAEMNADARRDPASLTKMMTSYVIGQALKAGKIGQE
EECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DMVTVGQDAWATGNPVFKGSSLMFLKPGDRVPVSKLTRGINLQSGNDACVAMADYVAGSQ
CCEEECCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEHHHHCCCH
DAFVNLMNTYVSKLGLQNTHFQTVHGLDAQGQYSSARDMALIGQALIRDVPDEYAIYKEK
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHEEECC
EFTFNNIRQMNRNGLLWDTSLNVDGIKTGHTDAAGYNLVASATEGQMRLISAVLGGRTYK
CCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCC
GRETESKKLLTWGFRFFETVAPLKVGKEFASEPVWFGDADRVELGVDKDVYLTIPRGRMK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEECCCHHH
DLKASYVLNTPEIHAPLAKNQVVGSINFQLDGKTIDQRPLVVMNEVKEGGFFSRIVDYIK
HCCHHEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEHHHHCCCHHHHHHHHHH
LMFHHWFG
HHHHHHCC
>Mature Secondary Structure 
PAGISSVIVWLVFNFTLMDVVVSIMKQVTSFRLIKSIALGTVIAMSATSVAHADDVNIK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TMIPGVPQIDAESYVLIDYNSGKVLAEMNADARRDPASLTKMMTSYVIGQALKAGKIGQE
EECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DMVTVGQDAWATGNPVFKGSSLMFLKPGDRVPVSKLTRGINLQSGNDACVAMADYVAGSQ
CCEEECCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEHHHHCCCH
DAFVNLMNTYVSKLGLQNTHFQTVHGLDAQGQYSSARDMALIGQALIRDVPDEYAIYKEK
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHEEECC
EFTFNNIRQMNRNGLLWDTSLNVDGIKTGHTDAAGYNLVASATEGQMRLISAVLGGRTYK
CCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCC
GRETESKKLLTWGFRFFETVAPLKVGKEFASEPVWFGDADRVELGVDKDVYLTIPRGRMK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEECCCHHH
DLKASYVLNTPEIHAPLAKNQVVGSINFQLDGKTIDQRPLVVMNEVKEGGFFSRIVDYIK
HCCHHEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEHHHHCCCHHHHHHHHHH
LMFHHWFG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]