Click here to switch to the map view.

The map label for this gene is lipB

Identifier: 157369439

GI number: 157369439

Start: 1312730

End: 1313398

Strand: Reverse

Name: lipB

Synonym: Spro_1196

Alternate gene names: 157369439

Gene position: 1313398-1312730 (Counterclockwise)

Preceding gene: 157369440

Following gene: 157369438

Centisome position: 24.1

GC content: 55.75

Gene sequence:

>669_bases
ATGACTCTTTTGCAACCAGACAAGATCATTTTGCGTCAGCTGGGGTTGCAGCCCTATGAGCCTGTATCCCAAGCCATGCA
CAACTTCACCGATCGCCGAACCGAAACCACGCCCGATGAGCTGTGGCTGGTTCAGCATCAGCCCGTATTTACCCAGGGCC
AGGCCGGTAAAGCAGAGCATCTGTTAATGCCGGGCGACATTCCGGTTGTCCAGAGCGATCGCGGTGGCCAGGTAACCTAC
CATGGGCCGGGTCAGCAGGTGATGTACGTGATGATCGATCTGAAACGCAACAAGGTTGGCGTTCGCCAACTGGTCACGGC
TATCGAAGATACCGTTATTAACACCCTCGCCCACTTTCACATTGAATCGCGCGCCCGTCCGGATGCGCCCGGTGTTTACG
TGGGGGAGCAAAAAATCTGTTCGTTGGGTTTGCGGATCCGTAAAGGCAGTTCATTCCACGGCCTGGCCCTGAATGTGGCC
ATGGATCTCAGCCCCTTCCAGCGTATCAACCCTTGCGGTTACGCCGGCATGCAGATGGCGCAGGTCAGCGCGCTGGCACC
CGGTGTTGGCATAGAAGACGTACACCCAGTCCTGGTACAGGAATTTGTTCATTTACTCGGCTACCCGAAGGTCGAGCTTC
GTAACTGGAACCTGCACGATTATGAGTAA

Upstream 100 bases:

>100_bases
AGAGCATCCTCTGCCCGGGTTGCGCATTTTTGGTCAGCGAAATGCTGGCCGCGGGCCAGAGCGCCCGGTATAATGGCGAC
ACCACTTCTGTAACCTGACG

Downstream 100 bases:

>100_bases
ACCGATTCAGATGGAACGCGGCGTTAAATACCGCGATGCAGACAAAATGGCTTTGATCCCGGTTAAAACGGTGGTCACTG
AACGGCAGGAGCTGTTACGT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MTLLQPDKIILRQLGLQPYEPVSQAMHNFTDRRTETTPDELWLVQHQPVFTQGQAGKAEHLLMPGDIPVVQSDRGGQVTY
HGPGQQVMYVMIDLKRNKVGVRQLVTAIEDTVINTLAHFHIESRARPDAPGVYVGEQKICSLGLRIRKGSSFHGLALNVA
MDLSPFQRINPCGYAGMQMAQVSALAPGVGIEDVHPVLVQEFVHLLGYPKVELRNWNLHDYE

Sequences:

>Translated_222_residues
MTLLQPDKIILRQLGLQPYEPVSQAMHNFTDRRTETTPDELWLVQHQPVFTQGQAGKAEHLLMPGDIPVVQSDRGGQVTY
HGPGQQVMYVMIDLKRNKVGVRQLVTAIEDTVINTLAHFHIESRARPDAPGVYVGEQKICSLGLRIRKGSSFHGLALNVA
MDLSPFQRINPCGYAGMQMAQVSALAPGVGIEDVHPVLVQEFVHLLGYPKVELRNWNLHDYE
>Mature_221_residues
TLLQPDKIILRQLGLQPYEPVSQAMHNFTDRRTETTPDELWLVQHQPVFTQGQAGKAEHLLMPGDIPVVQSDRGGQVTYH
GPGQQVMYVMIDLKRNKVGVRQLVTAIEDTVINTLAHFHIESRARPDAPGVYVGEQKICSLGLRIRKGSSFHGLALNVAM
DLSPFQRINPCGYAGMQMAQVSALAPGVGIEDVHPVLVQEFVHLLGYPKVELRNWNLHDYE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=134, Percent_Identity=35.0746268656716, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI87081767, Length=206, Percent_Identity=70.3883495145631, Blast_Score=313, Evalue=8e-87,
Organism=Saccharomyces cerevisiae, GI6323268, Length=158, Percent_Identity=33.5443037974684, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355483, Length=172, Percent_Identity=30.2325581395349, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_SERP5 (A8GB10)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477428.1
- ProteinModelPortal:   A8GB10
- SMR:   A8GB10
- STRING:   A8GB10
- GeneID:   5602900
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1196
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   GDEIWVV
- ProtClustDB:   PRK14342
- BioCyc:   SPRO399741:SPRO_1196-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24807; Mature: 24676

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLQPDKIILRQLGLQPYEPVSQAMHNFTDRRTETTPDELWLVQHQPVFTQGQAGKAEH
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHEEEEECCCEEECCCCCCCCE
LLMPGDIPVVQSDRGGQVTYHGPGQQVMYVMIDLKRNKVGVRQLVTAIEDTVINTLAHFH
EECCCCCCEEECCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IESRARPDAPGVYVGEQKICSLGLRIRKGSSFHGLALNVAMDLSPFQRINPCGYAGMQMA
HHCCCCCCCCCEEECHHHHHHHCEEEECCCCCCCEEEEEEECCCCHHHCCCCCCCHHHHH
QVSALAPGVGIEDVHPVLVQEFVHLLGYPKVELRNWNLHDYE
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCC
>Mature Secondary Structure 
TLLQPDKIILRQLGLQPYEPVSQAMHNFTDRRTETTPDELWLVQHQPVFTQGQAGKAEH
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHEEEEECCCEEECCCCCCCCE
LLMPGDIPVVQSDRGGQVTYHGPGQQVMYVMIDLKRNKVGVRQLVTAIEDTVINTLAHFH
EECCCCCCEEECCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IESRARPDAPGVYVGEQKICSLGLRIRKGSSFHGLALNVAMDLSPFQRINPCGYAGMQMA
HHCCCCCCCCCEEECHHHHHHHCEEEECCCCCCCEEEEEEECCCCHHHCCCCCCCHHHHH
QVSALAPGVGIEDVHPVLVQEFVHLLGYPKVELRNWNLHDYE
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA