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The map label for this gene is tesA [H]

Identifier: 157369400

GI number: 157369400

Start: 1273131

End: 1273721

Strand: Reverse

Name: tesA [H]

Synonym: Spro_1157

Alternate gene names: 157369400

Gene position: 1273721-1273131 (Counterclockwise)

Preceding gene: 157369405

Following gene: 157369399

Centisome position: 23.38

GC content: 57.02

Gene sequence:

>591_bases
TTGGGATTATTCAGTTTACGCGCTGTCGCCGCCGATACCTTATTGATATTAGGCGACAGTTTAAGCGCCGGTTACCGCTT
GCCGGTGGCGCAAGCCTGGCCGACGCTGCTGGCCGACCAATGGCAGAAAAAACCGGGTGACCCGCAACTGGTCAACGCCA
GCATCAGTGGCGATACCGCCGCTCAGGGGCTGGCACGTCTGCCCGAGCTGCTGAAACAACATCAGCCACGCTGGGTACTG
ATTGAACTGGGTGCCAACGACGGTCTGCGCGGTTTCCCGGCGCAGGATCTGCAACGCGACCTCAGCCAAATCATCACGCT
GGTGCAACAAGCCGGCGCGCAGCCGCTGCTGATGCAAATCCGTATTCCGCCCAACTATGGCCGCCGCTATACCGAGGCCT
TCAGTGCGATTTACCCGCAGCTTGCCAAACAATTCGACATTCCACTGCTGCCGTTCTATATGGAACAGGTGGTGGTGAAA
GCGGAATGGATGCAGGATGACGGATTGCATCCCAATAAGGATGCCCAACCGTTTATCGCCACCTGGATGGCGGAACGCCT
GGAACCTCTAGTAAAACATGAGTCTAACTAA

Upstream 100 bases:

>100_bases
CGGTAAGGATGGAGAGCTGATGCTCACCCTGACCAACGTGTTTACTAAGATGATGAACTTCAAGAACGTTTTCCGCTGGC
ATCTTCCCTTCCTTTTGCTG

Downstream 100 bases:

>100_bases
ATAGGGTTGTACACTAGGTAAAGTTATGCAAAAAGCAGTATTGATAACCGGCTGTTCCAGCGGGATTGGATTGATTGCCG
CGCAGGATCTGCGTAACCGT

Product: multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MGLFSLRAVAADTLLILGDSLSAGYRLPVAQAWPTLLADQWQKKPGDPQLVNASISGDTAAQGLARLPELLKQHQPRWVL
IELGANDGLRGFPAQDLQRDLSQIITLVQQAGAQPLLMQIRIPPNYGRRYTEAFSAIYPQLAKQFDIPLLPFYMEQVVVK
AEWMQDDGLHPNKDAQPFIATWMAERLEPLVKHESN

Sequences:

>Translated_196_residues
MGLFSLRAVAADTLLILGDSLSAGYRLPVAQAWPTLLADQWQKKPGDPQLVNASISGDTAAQGLARLPELLKQHQPRWVL
IELGANDGLRGFPAQDLQRDLSQIITLVQQAGAQPLLMQIRIPPNYGRRYTEAFSAIYPQLAKQFDIPLLPFYMEQVVVK
AEWMQDDGLHPNKDAQPFIATWMAERLEPLVKHESN
>Mature_195_residues
GLFSLRAVAADTLLILGDSLSAGYRLPVAQAWPTLLADQWQKKPGDPQLVNASISGDTAAQGLARLPELLKQHQPRWVLI
ELGANDGLRGFPAQDLQRDLSQIITLVQQAGAQPLLMQIRIPPNYGRRYTEAFSAIYPQLAKQFDIPLLPFYMEQVVVKA
EWMQDDGLHPNKDAQPFIATWMAERLEPLVKHESN

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=193, Percent_Identity=69.4300518134715, Blast_Score=281, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 21891; Mature: 21760

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLFSLRAVAADTLLILGDSLSAGYRLPVAQAWPTLLADQWQKKPGDPQLVNASISGDTA
CCCHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHH
AQGLARLPELLKQHQPRWVLIELGANDGLRGFPAQDLQRDLSQIITLVQQAGAQPLLMQI
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
RIPPNYGRRYTEAFSAIYPQLAKQFDIPLLPFYMEQVVVKAEWMQDDGLHPNKDAQPFIA
EECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
TWMAERLEPLVKHESN
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GLFSLRAVAADTLLILGDSLSAGYRLPVAQAWPTLLADQWQKKPGDPQLVNASISGDTA
CCHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHH
AQGLARLPELLKQHQPRWVLIELGANDGLRGFPAQDLQRDLSQIITLVQQAGAQPLLMQI
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
RIPPNYGRRYTEAFSAIYPQLAKQFDIPLLPFYMEQVVVKAEWMQDDGLHPNKDAQPFIA
EECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
TWMAERLEPLVKHESN
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]