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The map label for this gene is priC [H]

Identifier: 157369375

GI number: 157369375

Start: 1247068

End: 1247604

Strand: Reverse

Name: priC [H]

Synonym: Spro_1132

Alternate gene names: 157369375

Gene position: 1247604-1247068 (Counterclockwise)

Preceding gene: 157369388

Following gene: 157369374

Centisome position: 22.9

GC content: 56.42

Gene sequence:

>537_bases
GTGAGTACTCAGCGTCTTTTACAAGTGTTAGAACAACAGATCACGGTGTTAGCCGGCGAAATCACACCGCAAGGCGACGC
ACCGATCCCTCAGGCACGCTTTGACGCTGCGTTATTTTCCAACCGCGGCACCCGGCTGCGCGATTACCTGACAGAAGTAC
AAAAGAACTTCGCTCAGTTGCAGACCGCAGCCAACGACAGCCGCACCACCCAGGTGGCCTTTCTGGCCGAGAGACTGGTG
GCGCAGATCACCGCCCTGCAGCGCGAATTGGCGACCCAGGCACTGCGCCGAAAAAATCAGCCCAAGGAAGTGGTGGCGGC
CGATTTGTACCATAAGCTGGCTGAACATCAGGATTATGAGCGTCGGATTATTTCGATGATCCAGGATCGGGAAAGCCTGT
TGGGCAGACAGACCACGCTGGCAGCGCAGCAAAAACTGCAGCATGAGCTGGCGGCGCTGGAAGGCCGATTGATGCGTTGC
CGCCAGGCGTTGGCACGAATCGAGCGCAGTATTGAACGTAAAGAAAACGGTTTTTGA

Upstream 100 bases:

>100_bases
CCAGCGTCACCAGTCGCAACGGCCGGAATATGCGTGCAGCGCTTGTTTCACGGTGTGAACGCGTGCAAGCTGAGCCCACT
ATCCTCGTCATGGAGCCAAC

Downstream 100 bases:

>100_bases
AGATTTATCACAATATTTTAATGATTCTTCCCCTCCGCGGATCTTGTTCACTATACTCAGCCGAAGCGAAGCATCACTCA
TTCATCTCAATTTAAACGTG

Product: primosomal replication priB and priC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MSTQRLLQVLEQQITVLAGEITPQGDAPIPQARFDAALFSNRGTRLRDYLTEVQKNFAQLQTAANDSRTTQVAFLAERLV
AQITALQRELATQALRRKNQPKEVVAADLYHKLAEHQDYERRIISMIQDRESLLGRQTTLAAQQKLQHELAALEGRLMRC
RQALARIERSIERKENGF

Sequences:

>Translated_178_residues
MSTQRLLQVLEQQITVLAGEITPQGDAPIPQARFDAALFSNRGTRLRDYLTEVQKNFAQLQTAANDSRTTQVAFLAERLV
AQITALQRELATQALRRKNQPKEVVAADLYHKLAEHQDYERRIISMIQDRESLLGRQTTLAAQQKLQHELAALEGRLMRC
RQALARIERSIERKENGF
>Mature_177_residues
STQRLLQVLEQQITVLAGEITPQGDAPIPQARFDAALFSNRGTRLRDYLTEVQKNFAQLQTAANDSRTTQVAFLAERLVA
QITALQRELATQALRRKNQPKEVVAADLYHKLAEHQDYERRIISMIQDRESLLGRQTTLAAQQKLQHELAALEGRLMRCR
QALARIERSIERKENGF

Specific function: PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase [H]

COG id: COG3923

COG function: function code L; Primosomal replication protein N''

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786673, Length=174, Percent_Identity=39.6551724137931, Blast_Score=103, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010890 [H]

Pfam domain/function: PF07445 priB_priC [H]

EC number: NA

Molecular weight: Translated: 20336; Mature: 20204

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQRLLQVLEQQITVLAGEITPQGDAPIPQARFDAALFSNRGTRLRDYLTEVQKNFAQL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QTAANDSRTTQVAFLAERLVAQITALQRELATQALRRKNQPKEVVAADLYHKLAEHQDYE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHH
RRIISMIQDRESLLGRQTTLAAQQKLQHELAALEGRLMRCRQALARIERSIERKENGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STQRLLQVLEQQITVLAGEITPQGDAPIPQARFDAALFSNRGTRLRDYLTEVQKNFAQL
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QTAANDSRTTQVAFLAERLVAQITALQRELATQALRRKNQPKEVVAADLYHKLAEHQDYE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHH
RRIISMIQDRESLLGRQTTLAAQQKLQHELAALEGRLMRCRQALARIERSIERKENGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1856227; 9278503 [H]