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The map label for this gene is fixJ [H]

Identifier: 157369308

GI number: 157369308

Start: 1172318

End: 1172938

Strand: Reverse

Name: fixJ [H]

Synonym: Spro_1065

Alternate gene names: 157369308

Gene position: 1172938-1172318 (Counterclockwise)

Preceding gene: 157369309

Following gene: 157369302

Centisome position: 21.53

GC content: 56.84

Gene sequence:

>621_bases
ATGGCGTTAATACATCTGGTCGATGACGATTTAGCCGTTACCGACGCCTGCCGCTTTTTACTCGAGGGGCTGAACTATCG
GGTGCAGATCTGGCACGACAGCCAAAAGTTCGTCGACCAGGTCAGTCTTTATCAATGCGGTGTGGTGATGCTGGATATTC
GGATGCCGGGCATGGACGGGCAACAGGTTCATCAACAACTGCGTCGGCAGGAAAGTACCCTGGCGGTGGTGATCGTCACC
GGTCACGGTGATATTGCCATGGCCGTGACGGAGATGAAGCTGGGGGCCGTGGATTTCCTGCAAAAACCGATTGCCGCCGC
GCCGCTGATCGAAGCGCTGGATCGCGGGATCGCCCATTCTCAAAACCGGTTGGAACGCTATCAGCTACAGCAGCGTTTCA
GTGCGCTGACGCCGCGTGAGCGTGAAATAGCCCGCGCCGTGGCTGCGGGAATGACCAATAAACTGATCGCCGATCGTCAC
TTTATCGCGGTGCGTACCGTTGAAGTGCATCGGGCCAGAGTGATGGAAAAAATGCAGGCCGCCAGCCTGGCGGAGCTGGT
GAATTCACTCAATCAACTGACGTTAACCGAACCCTCAGAGAGTGGAGTCAGCCGCCTGTAG

Upstream 100 bases:

>100_bases
CAGGGCGGCGATCTGACACTGGAAAACTATTTTTCCGCTTCTCAACAGCAAGGGTTGCGCGTGACCCTGTACTTCCCCGT
GATCCAACAGGAAACCTGCC

Downstream 100 bases:

>100_bases
GCAAAAAAAATGCCGGTCGGAATAAGCCGACCGGCATCGTATTTCAACTCACGGGCAAATCACGGCATAATCGAAGGCTG
ATCTGCGCCCTCTTTTTCCA

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MALIHLVDDDLAVTDACRFLLEGLNYRVQIWHDSQKFVDQVSLYQCGVVMLDIRMPGMDGQQVHQQLRRQESTLAVVIVT
GHGDIAMAVTEMKLGAVDFLQKPIAAAPLIEALDRGIAHSQNRLERYQLQQRFSALTPREREIARAVAAGMTNKLIADRH
FIAVRTVEVHRARVMEKMQAASLAELVNSLNQLTLTEPSESGVSRL

Sequences:

>Translated_206_residues
MALIHLVDDDLAVTDACRFLLEGLNYRVQIWHDSQKFVDQVSLYQCGVVMLDIRMPGMDGQQVHQQLRRQESTLAVVIVT
GHGDIAMAVTEMKLGAVDFLQKPIAAAPLIEALDRGIAHSQNRLERYQLQQRFSALTPREREIARAVAAGMTNKLIADRH
FIAVRTVEVHRARVMEKMQAASLAELVNSLNQLTLTEPSESGVSRL
>Mature_205_residues
ALIHLVDDDLAVTDACRFLLEGLNYRVQIWHDSQKFVDQVSLYQCGVVMLDIRMPGMDGQQVHQQLRRQESTLAVVIVTG
HGDIAMAVTEMKLGAVDFLQKPIAAAPLIEALDRGIAHSQNRLERYQLQQRFSALTPREREIARAVAAGMTNKLIADRHF
IAVRTVEVHRARVMEKMQAASLAELVNSLNQLTLTEPSESGVSRL

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788712, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=27.906976744186, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=119, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23039; Mature: 22908

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIHLVDDDLAVTDACRFLLEGLNYRVQIWHDSQKFVDQVSLYQCGVVMLDIRMPGMDG
CEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCEEEEEEECCCCCH
QQVHQQLRRQESTLAVVIVTGHGDIAMAVTEMKLGAVDFLQKPIAAAPLIEALDRGIAHS
HHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QNRLERYQLQQRFSALTPREREIARAVAAGMTNKLIADRHFIAVRTVEVHRARVMEKMQA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHH
ASLAELVNSLNQLTLTEPSESGVSRL
HHHHHHHHHHHHEEECCCCCCCCCCC
>Mature Secondary Structure 
ALIHLVDDDLAVTDACRFLLEGLNYRVQIWHDSQKFVDQVSLYQCGVVMLDIRMPGMDG
EEEEEECCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCEEEEEEECCCCCH
QQVHQQLRRQESTLAVVIVTGHGDIAMAVTEMKLGAVDFLQKPIAAAPLIEALDRGIAHS
HHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QNRLERYQLQQRFSALTPREREIARAVAAGMTNKLIADRHFIAVRTVEVHRARVMEKMQA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHH
ASLAELVNSLNQLTLTEPSESGVSRL
HHHHHHHHHHHHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]