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The map label for this gene is nqrF

Identifier: 157369203

GI number: 157369203

Start: 1055846

End: 1057069

Strand: Direct

Name: nqrF

Synonym: Spro_0958

Alternate gene names: 157369203

Gene position: 1055846-1057069 (Clockwise)

Preceding gene: 157369202

Following gene: 157369204

Centisome position: 19.38

GC content: 54.41

Gene sequence:

>1224_bases
ATGGAAATTATTTTAGGCGTCGCGATGTTTACCGGCATCGTTATGGTGTTGGTTTTGCTCATCCTGTTCGCCAAATCCAG
GCTGGTGAACACCGGTGACATTGCGGTAGAAGTTAATGGCGATCTCGACAAGAGCTTTACCGCTCCGGCGGGTGACAAGC
TGCTGAACATGCTCTCCAGCCAGGGGATCTTCGTTTCGTCCGCCTGTGGTGGCGGCGGTTCTTGCGGCCAGTGCCGGGTG
GTGATCAAAGAGGGCGGCGGCGATATCCTGCCGACCGAACTGTCGCACATCAACAAGCGTGAAGCGAAAGAGGGCTGCCG
ACTGGCTTGTCAGGTGAACGTGAAACAGAACCTGAAAATCGAGCTGCCGGAAGAGATCTTCGGGGTGAAAAAGTGGGAGT
GCGAGGTTATCTCCAATGATAACAAAGCCACTTTTATCAAAGAGCTGAAACTGAAAATCCCTGACGGGGAAGACGTGCCG
TTCCGCGCCGGTGGGTTTATCCAGATCGAAGCGCCGGCACACGACATCAGCTACGCCGATTTTGACGTGCCTGACGAGTA
CCGTGGCGATTGGGACAAGTTCAACCTGTTCCGCTACCGCTCGGTAGTGAATGAAACCACGGTACGTGCCTACTCGATGG
CCAACTACCCGGACGAAAAGGGCATTATTATGCTCAACGTGCGTATTGCCACGCCGCCACCGCGCGATCCTGACGTGCCA
CCGGGCATCATGTCCTCTTATATCTGGTCGTTAAAGGCCGGGGACAAGGTGACCATTTCCGGGCCGTTCGGCGAGTTCTT
TGCCAAGGACACCGACGCGGAAATGATCTTTATCGGCGGCGGTGCCGGTATGGCGCCGATGCGTTCGCACATCTTTGACC
AGCTCAATCGCCTGAAGTCGAAGCGTAAAATCACCTTCTGGTACGGCGCGCGTTCACTGCGTGAGATGTTCTACGAGGAG
GATTTCAACCAACTGCAGGCAGAGAACGAGAACTTTACCTGGCATGTGGCGCTTTCCGATCCGCAGCCTGAGGATAACTG
GACGGGTTACACCGGCTTTATCCATAATGTTCTGTTGGAGAACTATCTGCGGAACCATCCGGCCCCGGAAGACTGTGAGT
TTTACATGTGCGGTCCGCCAATGATGAACGCCGCAGTGATCAAGATGCTGAAAGACCTGGGCGTTGAAGACGAAAATATC
ATGCTTGATGACTTTGGTGGCTAA

Upstream 100 bases:

>100_bases
AGGGTTGCGCGGCCTGGGCATTACCTTTATCACCACCGGGCTGATGGCGTTGGGCTTTATGTCCTTCTCCGGTGTTCAGT
TGTAAAGGCGGGAAGAATTT

Downstream 100 bases:

>100_bases
ATGCGCGCATGGGCAATGAAGGGGTTGATGACGACGGTGGCGCTCAGCGCCACCCTGTTATTGACCGGCTGCGGACCGGA
GCAGGTCAATCTCGATGGCA

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MEIILGVAMFTGIVMVLVLLILFAKSRLVNTGDIAVEVNGDLDKSFTAPAGDKLLNMLSSQGIFVSSACGGGGSCGQCRV
VIKEGGGDILPTELSHINKREAKEGCRLACQVNVKQNLKIELPEEIFGVKKWECEVISNDNKATFIKELKLKIPDGEDVP
FRAGGFIQIEAPAHDISYADFDVPDEYRGDWDKFNLFRYRSVVNETTVRAYSMANYPDEKGIIMLNVRIATPPPRDPDVP
PGIMSSYIWSLKAGDKVTISGPFGEFFAKDTDAEMIFIGGGAGMAPMRSHIFDQLNRLKSKRKITFWYGARSLREMFYEE
DFNQLQAENENFTWHVALSDPQPEDNWTGYTGFIHNVLLENYLRNHPAPEDCEFYMCGPPMMNAAVIKMLKDLGVEDENI
MLDDFGG

Sequences:

>Translated_407_residues
MEIILGVAMFTGIVMVLVLLILFAKSRLVNTGDIAVEVNGDLDKSFTAPAGDKLLNMLSSQGIFVSSACGGGGSCGQCRV
VIKEGGGDILPTELSHINKREAKEGCRLACQVNVKQNLKIELPEEIFGVKKWECEVISNDNKATFIKELKLKIPDGEDVP
FRAGGFIQIEAPAHDISYADFDVPDEYRGDWDKFNLFRYRSVVNETTVRAYSMANYPDEKGIIMLNVRIATPPPRDPDVP
PGIMSSYIWSLKAGDKVTISGPFGEFFAKDTDAEMIFIGGGAGMAPMRSHIFDQLNRLKSKRKITFWYGARSLREMFYEE
DFNQLQAENENFTWHVALSDPQPEDNWTGYTGFIHNVLLENYLRNHPAPEDCEFYMCGPPMMNAAVIKMLKDLGVEDENI
MLDDFGG
>Mature_407_residues
MEIILGVAMFTGIVMVLVLLILFAKSRLVNTGDIAVEVNGDLDKSFTAPAGDKLLNMLSSQGIFVSSACGGGGSCGQCRV
VIKEGGGDILPTELSHINKREAKEGCRLACQVNVKQNLKIELPEEIFGVKKWECEVISNDNKATFIKELKLKIPDGEDVP
FRAGGFIQIEAPAHDISYADFDVPDEYRGDWDKFNLFRYRSVVNETTVRAYSMANYPDEKGIIMLNVRIATPPPRDPDVP
PGIMSSYIWSLKAGDKVTISGPFGEFFAKDTDAEMIFIGGGAGMAPMRSHIFDQLNRLKSKRKITFWYGARSLREMFYEE
DFNQLQAENENFTWHVALSDPQPEDNWTGYTGFIHNVLLENYLRNHPAPEDCEFYMCGPPMMNAAVIKMLKDLGVEDENI
MLDDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI1788903, Length=228, Percent_Identity=26.7543859649123, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323552, Length=198, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322146, Length=240, Percent_Identity=25.8333333333333, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323510, Length=154, Percent_Identity=24.025974025974, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NQRF_SERP5 (A8GAC4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477192.1
- ProteinModelPortal:   A8GAC4
- SMR:   A8GAC4
- STRING:   A8GAC4
- GeneID:   5605182
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0958
- eggNOG:   COG2871
- HOGENOM:   HBG680293
- OMA:   VQLECPP
- ProtClustDB:   PRK05464
- BioCyc:   SPRO399741:SPRO_0958-MONOMER
- HAMAP:   MF_00430
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR00371
- TIGRFAMs:   TIGR01941

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1; SSF54292 Ferredoxin; SSF63380 Riboflavin_synthase_like_b-brl

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 45415; Mature: 45415

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2; PS51384 FAD_FR

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x229f4dc0)-;

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIILGVAMFTGIVMVLVLLILFAKSRLVNTGDIAVEVNGDLDKSFTAPAGDKLLNMLSS
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHC
QGIFVSSACGGGGSCGQCRVVIKEGGGDILPTELSHINKREAKEGCRLACQVNVKQNLKI
CCEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEE
ELPEEIFGVKKWECEVISNDNKATFIKELKLKIPDGEDVPFRAGGFIQIEAPAHDISYAD
ECCHHHHCCCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCC
FDVPDEYRGDWDKFNLFRYRSVVNETTVRAYSMANYPDEKGIIMLNVRIATPPPRDPDVP
CCCCHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEEECCCCCCCCCCC
PGIMSSYIWSLKAGDKVTISGPFGEFFAKDTDAEMIFIGGGAGMAPMRSHIFDQLNRLKS
HHHHHHHHHEECCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
KRKITFWYGARSLREMFYEEDFNQLQAENENFTWHVALSDPQPEDNWTGYTGFIHNVLLE
CCEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
NYLRNHPAPEDCEFYMCGPPMMNAAVIKMLKDLGVEDENIMLDDFGG
HHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCH
>Mature Secondary Structure
MEIILGVAMFTGIVMVLVLLILFAKSRLVNTGDIAVEVNGDLDKSFTAPAGDKLLNMLSS
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHC
QGIFVSSACGGGGSCGQCRVVIKEGGGDILPTELSHINKREAKEGCRLACQVNVKQNLKI
CCEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEE
ELPEEIFGVKKWECEVISNDNKATFIKELKLKIPDGEDVPFRAGGFIQIEAPAHDISYAD
ECCHHHHCCCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCC
FDVPDEYRGDWDKFNLFRYRSVVNETTVRAYSMANYPDEKGIIMLNVRIATPPPRDPDVP
CCCCHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEEECCCCCCCCCCC
PGIMSSYIWSLKAGDKVTISGPFGEFFAKDTDAEMIFIGGGAGMAPMRSHIFDQLNRLKS
HHHHHHHHHEECCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
KRKITFWYGARSLREMFYEEDFNQLQAENENFTWHVALSDPQPEDNWTGYTGFIHNVLLE
CCEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
NYLRNHPAPEDCEFYMCGPPMMNAAVIKMLKDLGVEDENIMLDDFGG
HHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA