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The map label for this gene is yafJ [H]

Identifier: 157369196

GI number: 157369196

Start: 1049234

End: 1050040

Strand: Direct

Name: yafJ [H]

Synonym: Spro_0951

Alternate gene names: 157369196

Gene position: 1049234-1050040 (Clockwise)

Preceding gene: 157369195

Following gene: 157369198

Centisome position: 19.26

GC content: 56.13

Gene sequence:

>807_bases
ATGTGTGAACTGCTCGGGATGAGCGCAAACGTACCGACCGATATTTGCTTTAGCTTTACCGGGCTGGTTCAGCGCGGTGG
CCGTACCGGGCCACATAAAGATGGTTGGGGCATTACCTTCTATGAAGGGAACGGTTGCCGCACGTTTAAGGATCCCCAGC
CGAGTTTCGACTCGCCGATCGCCCGTCTGGTGCAGGAATACCCGATCAAATCCTGCGCGGTGGTGTCGCATATCCGTCAG
GCCAATCGCGGTGAAGTGGCGCTGGAAAATACCCATCCCTTTACCCGTGAACTGTGGGGCCGTAACTGGACCTACGCGCA
CAATGGCCAGTTGAAAGGCTACCGTCAGTTGGAAACCGGCACCTTCCGCCCGGTTGGCCAGACTGACAGCGAATACGCTT
TCTGCTGGTTGCTGCATCAGCTTTCCCTGAAATACCCGCGCACCCCGAGCCGCTGGCCGCCGGTTTTCCGTTATATCGCT
TTGCTGGCGGATCAACTGCGTCAGAAGGGGGTATTCAATATGTTGCTGTCGGATGGGCGCTTTGTGATGGCTTATTGTTC
GACCAACCTGTATTGGATCACGCGTCGCGCGCCGTTTGGCAAAGCCACGTTACTCGATCAGGACGTAGAGATTGATTTTC
AGCAGCAGACCACGCCTAACGATGTGGTCACGGTGATTGCTACCCAGCCGCTGACGGCGAATGAAACCTGGCACAAGATT
GCGCCAGGTGAATTCGCACTATTTCACTTCGGTGAGCGGCTTGGTCTGAGCGAAGGGATTGGTACTGACAGTGGGAGCCG
TGGTTGA

Upstream 100 bases:

>100_bases
TAACGGCTTTAGGGGGCATATTGCCCCCGTTAATAATGCACCGGGGTGCGGCAGGTCGCGCCCTAATAAACGGTGTCAGT
GTGGTTAAGGAGTGCTGGCG

Downstream 100 bases:

>100_bases
CGCATACTGCGTCATCTGGCCGCTGCTCAGCACGGGCTGGCCCAACACGTACTGGCCATTGGCTACGCTGACCATCGGCG
GCTGGTGATTTTTGACGAAG

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MCELLGMSANVPTDICFSFTGLVQRGGRTGPHKDGWGITFYEGNGCRTFKDPQPSFDSPIARLVQEYPIKSCAVVSHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLKGYRQLETGTFRPVGQTDSEYAFCWLLHQLSLKYPRTPSRWPPVFRYIA
LLADQLRQKGVFNMLLSDGRFVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPNDVVTVIATQPLTANETWHKI
APGEFALFHFGERLGLSEGIGTDSGSRG

Sequences:

>Translated_268_residues
MCELLGMSANVPTDICFSFTGLVQRGGRTGPHKDGWGITFYEGNGCRTFKDPQPSFDSPIARLVQEYPIKSCAVVSHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLKGYRQLETGTFRPVGQTDSEYAFCWLLHQLSLKYPRTPSRWPPVFRYIA
LLADQLRQKGVFNMLLSDGRFVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPNDVVTVIATQPLTANETWHKI
APGEFALFHFGERLGLSEGIGTDSGSRG
>Mature_268_residues
MCELLGMSANVPTDICFSFTGLVQRGGRTGPHKDGWGITFYEGNGCRTFKDPQPSFDSPIARLVQEYPIKSCAVVSHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLKGYRQLETGTFRPVGQTDSEYAFCWLLHQLSLKYPRTPSRWPPVFRYIA
LLADQLRQKGVFNMLLSDGRFVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPNDVVTVIATQPLTANETWHKI
APGEFALFHFGERLGLSEGIGTDSGSRG

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=254, Percent_Identity=85.0393700787402, Blast_Score=462, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 30274; Mature: 30274

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGRTGPHKDGWGITFYEGNGCRTFKDPQPSFDSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
ARLVQEYPIKSCAVVSHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLKGYRQLETG
HHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCEEEECCC
TFRPVGQTDSEYAFCWLLHQLSLKYPRTPSRWPPVFRYIALLADQLRQKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC
FVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPNDVVTVIATQPLTANETWHKI
EEEEEECCCEEEEEECCCCCCCEEECCCCCEEECCCCCCCCEEEEEEECCCCCCCCEEEC
APGEFALFHFGERLGLSEGIGTDSGSRG
CCCCEEEEECHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGRTGPHKDGWGITFYEGNGCRTFKDPQPSFDSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
ARLVQEYPIKSCAVVSHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLKGYRQLETG
HHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCEEEECCC
TFRPVGQTDSEYAFCWLLHQLSLKYPRTPSRWPPVFRYIALLADQLRQKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC
FVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPNDVVTVIATQPLTANETWHKI
EEEEEECCCEEEEEECCCCCCCEEECCCCCEEECCCCCCCCEEEEEEECCCCCCCCEEEC
APGEFALFHFGERLGLSEGIGTDSGSRG
CCCCEEEEECHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]