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The map label for this gene is mltD [H]

Identifier: 157369153

GI number: 157369153

Start: 1007395

End: 1008822

Strand: Reverse

Name: mltD [H]

Synonym: Spro_0908

Alternate gene names: 157369153

Gene position: 1008822-1007395 (Counterclockwise)

Preceding gene: 157369154

Following gene: 157369150

Centisome position: 18.51

GC content: 51.61

Gene sequence:

>1428_bases
ATGATTGCTCGTCTTTTAAGCAACTACATTGACACACACATGAAGGCTAAAGCGATATTTCTCGCCTCAGTCTTGCTAGT
GGGGTGCCAGTCGTCCAGGCAGGACGCACCGGCCCCAGAACAGCATGCACAGAGTTTGTCTTCGGCAGGTCAAGAAGGTG
AAGCAGGAGAGTACACAGCGAATGGTCGAGCGAGCTCGGCGCGGTGGCTAGATAACAATAGTGGTGCCGCGCAACAGAAC
CTGTGGAACTTCATTAGCGACGAGCTGAAGATGGAGGTTCCGGAAAATTCCCGGATCCGTGATCAAAAAAGAAAGTACCT
AAAAAGTAAGAGCTATCTCCACGATGTAACATTACGGGCAGAGCCGTACATGTACTGGATAGTCGGGCAGATTAAGAAAC
GCAATATGCCGATGGAACTGGTACTGCTACCCATAGTGGAGAGCGCTTTTGACCCCCACGCCACGTCAAGTGCCAACGCC
GCAGGGCTATGGCAAATCGTGCCGCAAACGGGTCGGAATTATGGTTTGAAAAACAACCAGTGGTATGACGGGCGACGCGA
TGTCGTGGCCTCGACGACTGCTGCGCTTGATATGATGCAGTATTTGAACCGCATGTTTAACGGTGACTGGTTACTGACCA
TTGCCGCTTATAACAGTGGTGAAGGCCGTGTCATGCAGGCGGTTAAGGCGAATAAACGCCAGGGTAAGCCAACCAATTTC
TGGGCATTGTCGCTTCCGCGTGAAACGTCAATTTATGTCCCGAAAATGCTGGCGCTGAGCGACATCATTAAAAACAGCAA
GAAGTACGGTATCAATCTGCCTAAGACTGATAACACCCGTGCACTGGCGCGTATTGATGTTGGACAGCAAATACAGTTAA
CTCAGGCGGCTGAGATGGCTGGGCTTTCATTGACCAAGATGAAGGCTTATAACCCAGGCTACAAAAAAGGCGTTACGGCA
CCCAACGGGCCCCATTATATTATGGTTCCCAAAGGTCATGCCGATCAGCTGAAAGACTCGTTGGCCGATGGCCAGATCGC
AGTGACTCAGCCAACCACTCAGTTGGCGAAGAACAGCGGCCTGTCTGGTGGCAGCTCGTATAAAGTGCGCTCCGGCGACA
CGGTATCGGGCATTGCCAAGCGGTTGAATATCAAGTCCAGCGATTTGCAGAGTTGGAACAATTTGCGCGCCAAGAGCACG
CTAAAAGTTGGGCAAACCCTGCAAGTGGCCAGCAATACCGGCAGTAACAGCAGCATCACCTATCAGGTTCGTAAAGGTGA
TTCGCTCGCCAGTATTGCACGTCGTCACGGCGTCGATATTAACGACGTGATGCGTTGGAACTCAACGCTTGCCAAAGCCA
ACAGCAACCTGCAGCCGGGTCTGAAATTGACCCTGTTTGTCAGCGATAAAACGTCGCCGGATACCTAA

Upstream 100 bases:

>100_bases
TTCATACGCCACCAGAGCATCTTCATTCGGTTTTTTCCGAATTACGCCTGCGTAAAGACAACTTCTGAGCTTTTAGTTGT
GTTGTTTGGCGAAGCAAAGT

Downstream 100 bases:

>100_bases
ATTTTCGGCACAAAAAAAGCACCCTTCGGGGTGCTTTTTTATTTGTCTGAATATGGTGCGAATGTTACCAATGCGGCTTG
TCGGCTTCAGGGTGAAACTC

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MIARLLSNYIDTHMKAKAIFLASVLLVGCQSSRQDAPAPEQHAQSLSSAGQEGEAGEYTANGRASSARWLDNNSGAAQQN
LWNFISDELKMEVPENSRIRDQKRKYLKSKSYLHDVTLRAEPYMYWIVGQIKKRNMPMELVLLPIVESAFDPHATSSANA
AGLWQIVPQTGRNYGLKNNQWYDGRRDVVASTTAALDMMQYLNRMFNGDWLLTIAAYNSGEGRVMQAVKANKRQGKPTNF
WALSLPRETSIYVPKMLALSDIIKNSKKYGINLPKTDNTRALARIDVGQQIQLTQAAEMAGLSLTKMKAYNPGYKKGVTA
PNGPHYIMVPKGHADQLKDSLADGQIAVTQPTTQLAKNSGLSGGSSYKVRSGDTVSGIAKRLNIKSSDLQSWNNLRAKST
LKVGQTLQVASNTGSNSSITYQVRKGDSLASIARRHGVDINDVMRWNSTLAKANSNLQPGLKLTLFVSDKTSPDT

Sequences:

>Translated_475_residues
MIARLLSNYIDTHMKAKAIFLASVLLVGCQSSRQDAPAPEQHAQSLSSAGQEGEAGEYTANGRASSARWLDNNSGAAQQN
LWNFISDELKMEVPENSRIRDQKRKYLKSKSYLHDVTLRAEPYMYWIVGQIKKRNMPMELVLLPIVESAFDPHATSSANA
AGLWQIVPQTGRNYGLKNNQWYDGRRDVVASTTAALDMMQYLNRMFNGDWLLTIAAYNSGEGRVMQAVKANKRQGKPTNF
WALSLPRETSIYVPKMLALSDIIKNSKKYGINLPKTDNTRALARIDVGQQIQLTQAAEMAGLSLTKMKAYNPGYKKGVTA
PNGPHYIMVPKGHADQLKDSLADGQIAVTQPTTQLAKNSGLSGGSSYKVRSGDTVSGIAKRLNIKSSDLQSWNNLRAKST
LKVGQTLQVASNTGSNSSITYQVRKGDSLASIARRHGVDINDVMRWNSTLAKANSNLQPGLKLTLFVSDKTSPDT
>Mature_475_residues
MIARLLSNYIDTHMKAKAIFLASVLLVGCQSSRQDAPAPEQHAQSLSSAGQEGEAGEYTANGRASSARWLDNNSGAAQQN
LWNFISDELKMEVPENSRIRDQKRKYLKSKSYLHDVTLRAEPYMYWIVGQIKKRNMPMELVLLPIVESAFDPHATSSANA
AGLWQIVPQTGRNYGLKNNQWYDGRRDVVASTTAALDMMQYLNRMFNGDWLLTIAAYNSGEGRVMQAVKANKRQGKPTNF
WALSLPRETSIYVPKMLALSDIIKNSKKYGINLPKTDNTRALARIDVGQQIQLTQAAEMAGLSLTKMKAYNPGYKKGVTA
PNGPHYIMVPKGHADQLKDSLADGQIAVTQPTTQLAKNSGLSGGSSYKVRSGDTVSGIAKRLNIKSSDLQSWNNLRAKST
LKVGQTLQVASNTGSNSSITYQVRKGDSLASIARRHGVDINDVMRWNSTLAKANSNLQPGLKLTLFVSDKTSPDT

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=469, Percent_Identity=62.8997867803838, Blast_Score=588, Evalue=1e-169,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 52215; Mature: 52215

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARLLSNYIDTHMKAKAIFLASVLLVGCQSSRQDAPAPEQHAQSLSSAGQEGEAGEYTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEC
NGRASSARWLDNNSGAAQQNLWNFISDELKMEVPENSRIRDQKRKYLKSKSYLHDVTLRA
CCCCCCCEEECCCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHEEEEEEC
EPYMYWIVGQIKKRNMPMELVLLPIVESAFDPHATSSANAAGLWQIVPQTGRNYGLKNNQ
CCEEEEEEEHHHCCCCCCEEEEHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
WYDGRRDVVASTTAALDMMQYLNRMFNGDWLLTIAAYNSGEGRVMQAVKANKRQGKPTNF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCE
WALSLPRETSIYVPKMLALSDIIKNSKKYGINLPKTDNTRALARIDVGQQIQLTQAAEMA
EEEECCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHH
GLSLTKMKAYNPGYKKGVTAPNGPHYIMVPKGHADQLKDSLADGQIAVTQPTTQLAKNSG
CCEEEEEECCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEECCHHHHHHHCC
LSGGSSYKVRSGDTVSGIAKRLNIKSSDLQSWNNLRAKSTLKVGQTLQVASNTGSNSSIT
CCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCEEEEECCCCCCCEEE
YQVRKGDSLASIARRHGVDINDVMRWNSTLAKANSNLQPGLKLTLFVSDKTSPDT
EEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MIARLLSNYIDTHMKAKAIFLASVLLVGCQSSRQDAPAPEQHAQSLSSAGQEGEAGEYTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEC
NGRASSARWLDNNSGAAQQNLWNFISDELKMEVPENSRIRDQKRKYLKSKSYLHDVTLRA
CCCCCCCEEECCCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHEEEEEEC
EPYMYWIVGQIKKRNMPMELVLLPIVESAFDPHATSSANAAGLWQIVPQTGRNYGLKNNQ
CCEEEEEEEHHHCCCCCCEEEEHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
WYDGRRDVVASTTAALDMMQYLNRMFNGDWLLTIAAYNSGEGRVMQAVKANKRQGKPTNF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCE
WALSLPRETSIYVPKMLALSDIIKNSKKYGINLPKTDNTRALARIDVGQQIQLTQAAEMA
EEEECCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHH
GLSLTKMKAYNPGYKKGVTAPNGPHYIMVPKGHADQLKDSLADGQIAVTQPTTQLAKNSG
CCEEEEEECCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEECCHHHHHHHCC
LSGGSSYKVRSGDTVSGIAKRLNIKSSDLQSWNNLRAKSTLKVGQTLQVASNTGSNSSIT
CCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCEEEEECCCCCCCEEE
YQVRKGDSLASIARRHGVDINDVMRWNSTLAKANSNLQPGLKLTLFVSDKTSPDT
EEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]