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The map label for this gene is murF [H]

Identifier: 157369002

GI number: 157369002

Start: 844625

End: 845986

Strand: Direct

Name: murF [H]

Synonym: Spro_0757

Alternate gene names: 157369002

Gene position: 844625-845986 (Clockwise)

Preceding gene: 157369001

Following gene: 157369003

Centisome position: 15.5

GC content: 58.81

Gene sequence:

>1362_bases
ATGATCCCTGTCTCCCTTCGGACACTGGCCGATGTATTGAGCGCTGAGTTGATCGGCGCCGATTGCCAAATCGTTGAGGT
GACGACAGATACCCGTCAGGTAACCACAGGTTGCCTGTTTGTGGCACTGAAAGGCGAGCGTTTCGACGCTCACGATTTTG
CTGTTGATGCGGTAGCCGCTGGCGCCGGGGCACTGCTGGTAAGTAAGCGCTTATTGGTGGAGGTTCCGCAACTGGTGGTA
AAAGACACCCGCCTGGCACTGGGACAGCTCGCTGCCTGGGTTCGCCAGCAGGTGCCGACCCGCGTAGTGGCGTTAACCGG
TTCATCGGGCAAGACCTCGGTGAAGGAAATGGCCGCGGCCATTCTGCGTGAATGCGGTGAAGTGCTGTATACCGCCGGTA
ACTTCAACAACGATATCGGCGTGCCGCTGACGCTGTTGCGTTTGCAGCCACAGCATGACTTTGCCGTGATTGAACTGGGT
GCCAACCATATTGGCGAAATTGCTTACACCACCGCTTTAACCCGTCCGCAAACCGCCCTGGTGAATAACCTGGCGGCCGC
CCACCTGGAAGGCTTTGGCTCTTTGGCCGGTGTCGCTCAGGCGAAAGGCGAAATTTTTGCCGGTTTACCGGCTGACGGCG
TGGCGATCCTCAATGCCGACAACAATGACTGGCCGCACTGGCAGAGCATGCTGGACGGCAAAACCGTCTGGCGCTTCTCG
CCGCAGGCGGCAGAAGGCGTTGATTTCTTTGCCAGCGACGTGCAGGTGAACGGCAAGGGCACGCAGTTTAGGCTGCACAG
TCCGTTCGGCACCGCAGATATTGCACTGCCGTTGCCGGGTCGCCATAACGTAGCCAATGCGTTGGCCGCTGCGGCGCTGG
CCATGTCGGCCGGAGCATCGCTTGAGGCGGTGCGTCAGGGGCTCAAACAATTACAGGCGGTGCCGGGGCGTTTGTTCCCG
ATTGCACTTTCTGACCATCAGCTGCTGCTGGATGACAGCTATAACGCCAACGTCGGTTCAATGACCGCCGCCGCACAGGT
CCTGGCGGAAATGCCGGGCTACCGCGTGATGGTGGTGGGTGACATGGCAGAGTTGGGGGCAGAAGCCGAAGAGTGTCACC
GTCAGGTGGGCGAAGCCGCCCGGTTGGCAGGGGTCGACAAAGTATTGGGTGTCGGTCCGTTAAGCCAGGTGCTGACTGAA
GCTTCGGGTAATGGCGAACACTTTCAGGATAAGGCAGCAGTGATCGCGCGCGTGGCGGAATTACTGTCAGAACATGCGGT
AATTACCGTGTTAATCAAGGGTTCACGTAGTGCCGCAATGGAGCAGGTAGTACGCGCGTTACAGGAGAAAGCACCATGTT
AG

Upstream 100 bases:

>100_bases
TGGTGGCGGGTAAAGGCCACGAGGACTACCAACTGGTGGGGAATCGCCGTTTGGATTACTCCGATCGTACCACCGTAGCG
CGTCTGCTGGGGGTAGTAGC

Downstream 100 bases:

>100_bases
TTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTCAACGTCTTTTCCTACCTGACGTTCCGAGCCATTGTCAGC
CTGCTGACCGCGCTGTTCCT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MIPVSLRTLADVLSAELIGADCQIVEVTTDTRQVTTGCLFVALKGERFDAHDFAVDAVAAGAGALLVSKRLLVEVPQLVV
KDTRLALGQLAAWVRQQVPTRVVALTGSSGKTSVKEMAAAILRECGEVLYTAGNFNNDIGVPLTLLRLQPQHDFAVIELG
ANHIGEIAYTTALTRPQTALVNNLAAAHLEGFGSLAGVAQAKGEIFAGLPADGVAILNADNNDWPHWQSMLDGKTVWRFS
PQAAEGVDFFASDVQVNGKGTQFRLHSPFGTADIALPLPGRHNVANALAAAALAMSAGASLEAVRQGLKQLQAVPGRLFP
IALSDHQLLLDDSYNANVGSMTAAAQVLAEMPGYRVMVVGDMAELGAEAEECHRQVGEAARLAGVDKVLGVGPLSQVLTE
ASGNGEHFQDKAAVIARVAELLSEHAVITVLIKGSRSAAMEQVVRALQEKAPC

Sequences:

>Translated_453_residues
MIPVSLRTLADVLSAELIGADCQIVEVTTDTRQVTTGCLFVALKGERFDAHDFAVDAVAAGAGALLVSKRLLVEVPQLVV
KDTRLALGQLAAWVRQQVPTRVVALTGSSGKTSVKEMAAAILRECGEVLYTAGNFNNDIGVPLTLLRLQPQHDFAVIELG
ANHIGEIAYTTALTRPQTALVNNLAAAHLEGFGSLAGVAQAKGEIFAGLPADGVAILNADNNDWPHWQSMLDGKTVWRFS
PQAAEGVDFFASDVQVNGKGTQFRLHSPFGTADIALPLPGRHNVANALAAAALAMSAGASLEAVRQGLKQLQAVPGRLFP
IALSDHQLLLDDSYNANVGSMTAAAQVLAEMPGYRVMVVGDMAELGAEAEECHRQVGEAARLAGVDKVLGVGPLSQVLTE
ASGNGEHFQDKAAVIARVAELLSEHAVITVLIKGSRSAAMEQVVRALQEKAPC
>Mature_453_residues
MIPVSLRTLADVLSAELIGADCQIVEVTTDTRQVTTGCLFVALKGERFDAHDFAVDAVAAGAGALLVSKRLLVEVPQLVV
KDTRLALGQLAAWVRQQVPTRVVALTGSSGKTSVKEMAAAILRECGEVLYTAGNFNNDIGVPLTLLRLQPQHDFAVIELG
ANHIGEIAYTTALTRPQTALVNNLAAAHLEGFGSLAGVAQAKGEIFAGLPADGVAILNADNNDWPHWQSMLDGKTVWRFS
PQAAEGVDFFASDVQVNGKGTQFRLHSPFGTADIALPLPGRHNVANALAAAALAMSAGASLEAVRQGLKQLQAVPGRLFP
IALSDHQLLLDDSYNANVGSMTAAAQVLAEMPGYRVMVVGDMAELGAEAEECHRQVGEAARLAGVDKVLGVGPLSQVLTE
ASGNGEHFQDKAAVIARVAELLSEHAVITVLIKGSRSAAMEQVVRALQEKAPC

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=453, Percent_Identity=70.6401766004415, Blast_Score=629, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 47575; Mature: 47575

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVSLRTLADVLSAELIGADCQIVEVTTDTRQVTTGCLFVALKGERFDAHDFAVDAVAA
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCEEEEEEEECCCCCCHHHHHHHHHC
GAGALLVSKRLLVEVPQLVVKDTRLALGQLAAWVRQQVPTRVVALTGSSGKTSVKEMAAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
ILRECGEVLYTAGNFNNDIGVPLTLLRLQPQHDFAVIELGANHIGEIAYTTALTRPQTAL
HHHHHHHHEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCHHCHHHHHHHCCCCHHHH
VNNLAAAHLEGFGSLAGVAQAKGEIFAGLPADGVAILNADNNDWPHWQSMLDGKTVWRFS
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCHHHHHHCCCEEEEEC
PQAAEGVDFFASDVQVNGKGTQFRLHSPFGTADIALPLPGRHNVANALAAAALAMSAGAS
CCHHCCCHHEECCEEECCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
LEAVRQGLKQLQAVPGRLFPIALSDHQLLLDDSYNANVGSMTAAAQVLAEMPGYRVMVVG
HHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEE
DMAELGAEAEECHRQVGEAARLAGVDKVLGVGPLSQVLTEASGNGEHFQDKAAVIARVAE
CHHHHCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LLSEHAVITVLIKGSRSAAMEQVVRALQEKAPC
HHHCCCEEEEEEECCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIPVSLRTLADVLSAELIGADCQIVEVTTDTRQVTTGCLFVALKGERFDAHDFAVDAVAA
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCEEEEEEEECCCCCCHHHHHHHHHC
GAGALLVSKRLLVEVPQLVVKDTRLALGQLAAWVRQQVPTRVVALTGSSGKTSVKEMAAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
ILRECGEVLYTAGNFNNDIGVPLTLLRLQPQHDFAVIELGANHIGEIAYTTALTRPQTAL
HHHHHHHHEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCHHCHHHHHHHCCCCHHHH
VNNLAAAHLEGFGSLAGVAQAKGEIFAGLPADGVAILNADNNDWPHWQSMLDGKTVWRFS
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCHHHHHHCCCEEEEEC
PQAAEGVDFFASDVQVNGKGTQFRLHSPFGTADIALPLPGRHNVANALAAAALAMSAGAS
CCHHCCCHHEECCEEECCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
LEAVRQGLKQLQAVPGRLFPIALSDHQLLLDDSYNANVGSMTAAAQVLAEMPGYRVMVVG
HHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEE
DMAELGAEAEECHRQVGEAARLAGVDKVLGVGPLSQVLTEASGNGEHFQDKAAVIARVAE
CHHHHCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LLSEHAVITVLIKGSRSAAMEQVVRALQEKAPC
HHHCCCEEEEEEECCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]