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The map label for this gene is yfkN [H]

Identifier: 157368903

GI number: 157368903

Start: 722150

End: 723703

Strand: Direct

Name: yfkN [H]

Synonym: Spro_0658

Alternate gene names: 157368903

Gene position: 722150-723703 (Clockwise)

Preceding gene: 157368902

Following gene: 157368904

Centisome position: 13.25

GC content: 48.26

Gene sequence:

>1554_bases
GTGAAAATATCAAAATTTATCTTTCCATTAGTCACAGTGGCATTTTTTACCAGCGCCTCTGTTCAGGCTGTTGACCTTAC
GATATATCATACCAACGACTTGCATGCCAATGTGCTCCCTTTTAAAGCCCCTTATGTCAGTAAAGAAAAACCAGTGGGGG
GATTCGCCAATATTGCCACTATCGTCAAACAGGCCAAAGCCAAAGATGATGGCGTCTTCTTCTTCGATGCCGGGGATTTT
TTTACCGGTCCTTATATCAGCACGCTAACGAAGGGTGAGGCTATCATTGATGTGATGAATCAAATGTCGTTTGATGCTGT
TTCTATCGGTAATCATGAATTTGATCATGGTGTACCTAATATGATTCAGCAGATGAATAAGGCCACCTTTCCTGCGTTAT
TAGGCAACCTGTTCTATGAAAATAGCGATAAACCGGTGTGGGATAAACCCTGGGTTATTTTGGAGAAGGCCGGCGTTAAG
GTTGGCGTGATTGGTTTGCATGGGAAATTCGCTTTCTACGATACGGTAAATAAAAATATGCGCAAGGGTGTCGAGATGCG
CGACGAAGTGGAATACCTGAATAAATATCTTGGGCAGATCCGCGACAAGGTGGACATTACCGTATTGTTAATTCATGAAG
GCGTGCCGGCACGGCAATCCAGTTTTGGTGCGACGGACGTAAGTCGGAGGTTAAATAAAGACATAGAGTTGGCAAAGCAG
GTGCAGGGGCTGGATATCATTATTACCGGTCATGCTCATGTGGGTACGCCGGAACCCATCAAGGTGAACAATACATTAAT
CGTTTCCACCGATGCCTATGGCATCGATCTGGGAAAACTGGTGCTGAATATTGACGATAAAAGTAAAAAGATCACCGGCT
ATAAAGGGCAGCTAATCAATATTTACGCTGGTGATGTTGAACCCGATCGGGCAACGGCGGATAAGATCGCGCTATGGACG
GATAAGCTGAAAGAGATCACTCAGCAGAAAATCGGTAAGGCCAGCGCAGAATTGACGCGTTCTTATGGTGAGTCTTCTCC
CGTAGGCAATATGGTCGGGGATGCCTTTTTGGCCATGGATCCACGGGCGCAATTCGCCTTCGTCAACAGTGGCGGTTTGA
GAAGTGAGCTGGCTCAGGGGGAAATTAAACTGGGCGATATTATCACCCTGTTTCCTTTCCCGGACGACTTGAGCTACATG
GAAATCAACGGCAAACAGCTACGTTCGTTAATGAATCATGCGGCAAATCTGACCAATGGCATCCTGCAAGTCTCGAAGGG
CATCGAGATGAAATACGATAGCCGCAAGCCGCTCAATCAGCGCATCGTCTCGTTAACGGTGCAGGGGCAGCCGCTCAAGG
ATGACGCCTGGTACCCGATTGTGGTGAATTCATTCCTGGCCACGGGTGGGGATGGGTTCAGCGCCTTTACCGAAGGGCGT
AACATCAAGACGGTGGCCGGCAGCGGTGCGGCAGAAGCGGTGGTGGACTATATCAAGTCCCAAGCGGAGATCTCCCCCAA
CGAGCAAAAACGGATTAAGGACGTGGCACTGTAG

Upstream 100 bases:

>100_bases
TCAGGTATTACAATACACTTAACGCGTTTAAATAAAGGGTGATTCAAAAACCGCATATTGGATCAGGCTGTGCCCTACAC
ATGGATTGAGGGAATATTAC

Downstream 100 bases:

>100_bases
CCAATTAGGCCGATGAGCCAAGGTCATGCAGAAGGATTAAACCGTGACGCATGTCACGTACTGGTGTTTTCTATTGCGTC
TGGTTGTATATATTTGTGAC

Product: 5'-nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKISKFIFPLVTVAFFTSASVQAVDLTIYHTNDLHANVLPFKAPYVSKEKPVGGFANIATIVKQAKAKDDGVFFFDAGDF
FTGPYISTLTKGEAIIDVMNQMSFDAVSIGNHEFDHGVPNMIQQMNKATFPALLGNLFYENSDKPVWDKPWVILEKAGVK
VGVIGLHGKFAFYDTVNKNMRKGVEMRDEVEYLNKYLGQIRDKVDITVLLIHEGVPARQSSFGATDVSRRLNKDIELAKQ
VQGLDIIITGHAHVGTPEPIKVNNTLIVSTDAYGIDLGKLVLNIDDKSKKITGYKGQLINIYAGDVEPDRATADKIALWT
DKLKEITQQKIGKASAELTRSYGESSPVGNMVGDAFLAMDPRAQFAFVNSGGLRSELAQGEIKLGDIITLFPFPDDLSYM
EINGKQLRSLMNHAANLTNGILQVSKGIEMKYDSRKPLNQRIVSLTVQGQPLKDDAWYPIVVNSFLATGGDGFSAFTEGR
NIKTVAGSGAAEAVVDYIKSQAEISPNEQKRIKDVAL

Sequences:

>Translated_517_residues
MKISKFIFPLVTVAFFTSASVQAVDLTIYHTNDLHANVLPFKAPYVSKEKPVGGFANIATIVKQAKAKDDGVFFFDAGDF
FTGPYISTLTKGEAIIDVMNQMSFDAVSIGNHEFDHGVPNMIQQMNKATFPALLGNLFYENSDKPVWDKPWVILEKAGVK
VGVIGLHGKFAFYDTVNKNMRKGVEMRDEVEYLNKYLGQIRDKVDITVLLIHEGVPARQSSFGATDVSRRLNKDIELAKQ
VQGLDIIITGHAHVGTPEPIKVNNTLIVSTDAYGIDLGKLVLNIDDKSKKITGYKGQLINIYAGDVEPDRATADKIALWT
DKLKEITQQKIGKASAELTRSYGESSPVGNMVGDAFLAMDPRAQFAFVNSGGLRSELAQGEIKLGDIITLFPFPDDLSYM
EINGKQLRSLMNHAANLTNGILQVSKGIEMKYDSRKPLNQRIVSLTVQGQPLKDDAWYPIVVNSFLATGGDGFSAFTEGR
NIKTVAGSGAAEAVVDYIKSQAEISPNEQKRIKDVAL
>Mature_517_residues
MKISKFIFPLVTVAFFTSASVQAVDLTIYHTNDLHANVLPFKAPYVSKEKPVGGFANIATIVKQAKAKDDGVFFFDAGDF
FTGPYISTLTKGEAIIDVMNQMSFDAVSIGNHEFDHGVPNMIQQMNKATFPALLGNLFYENSDKPVWDKPWVILEKAGVK
VGVIGLHGKFAFYDTVNKNMRKGVEMRDEVEYLNKYLGQIRDKVDITVLLIHEGVPARQSSFGATDVSRRLNKDIELAKQ
VQGLDIIITGHAHVGTPEPIKVNNTLIVSTDAYGIDLGKLVLNIDDKSKKITGYKGQLINIYAGDVEPDRATADKIALWT
DKLKEITQQKIGKASAELTRSYGESSPVGNMVGDAFLAMDPRAQFAFVNSGGLRSELAQGEIKLGDIITLFPFPDDLSYM
EINGKQLRSLMNHAANLTNGILQVSKGIEMKYDSRKPLNQRIVSLTVQGQPLKDDAWYPIVVNSFLATGGDGFSAFTEGR
NIKTVAGSGAAEAVVDYIKSQAEISPNEQKRIKDVAL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=549, Percent_Identity=26.4116575591985, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1786687, Length=528, Percent_Identity=23.8636363636364, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790658, Length=611, Percent_Identity=23.0769230769231, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922446, Length=555, Percent_Identity=27.9279279279279, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24654424, Length=555, Percent_Identity=27.9279279279279, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28573524, Length=552, Percent_Identity=25.1811594202899, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922444, Length=558, Percent_Identity=25.9856630824373, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921980, Length=468, Percent_Identity=25.4273504273504, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652512, Length=468, Percent_Identity=25.4273504273504, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161076508, Length=465, Percent_Identity=25.5913978494624, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24641187, Length=533, Percent_Identity=25.328330206379, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI221329836, Length=563, Percent_Identity=23.9786856127886, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 56706; Mature: 56706

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISKFIFPLVTVAFFTSASVQAVDLTIYHTNDLHANVLPFKAPYVSKEKPVGGFANIAT
CCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
IVKQAKAKDDGVFFFDAGDFFTGPYISTLTKGEAIIDVMNQMSFDAVSIGNHEFDHGVPN
HHHHHCCCCCCEEEEECCCCCCCCHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCHHH
MIQQMNKATFPALLGNLFYENSDKPVWDKPWVILEKAGVKVGVIGLHGKFAFYDTVNKNM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEHHHHHHH
RKGVEMRDEVEYLNKYLGQIRDKVDITVLLIHEGVPARQSSFGATDVSRRLNKDIELAKQ
HCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCHHHHHHH
VQGLDIIITGHAHVGTPEPIKVNNTLIVSTDAYGIDLGKLVLNIDDKSKKITGYKGQLIN
CCCCEEEEECCCCCCCCCCEEECCEEEEECCCCCCEEEEEEEEECCCCCEECCCCCEEEE
IYAGDVEPDRATADKIALWTDKLKEITQQKIGKASAELTRSYGESSPVGNMVGDAFLAMD
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHEEEC
PRAQFAFVNSGGLRSELAQGEIKLGDIITLFPFPDDLSYMEINGKQLRSLMNHAANLTNG
CCCEEEEEECCCCHHHHHCCCEEECCEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHH
ILQVSKGIEMKYDSRKPLNQRIVSLTVQGQPLKDDAWYPIVVNSFLATGGDGFSAFTEGR
HHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCC
NIKTVAGSGAAEAVVDYIKSQAEISPNEQKRIKDVAL
CEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKISKFIFPLVTVAFFTSASVQAVDLTIYHTNDLHANVLPFKAPYVSKEKPVGGFANIAT
CCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
IVKQAKAKDDGVFFFDAGDFFTGPYISTLTKGEAIIDVMNQMSFDAVSIGNHEFDHGVPN
HHHHHCCCCCCEEEEECCCCCCCCHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCHHH
MIQQMNKATFPALLGNLFYENSDKPVWDKPWVILEKAGVKVGVIGLHGKFAFYDTVNKNM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEHHHHHHH
RKGVEMRDEVEYLNKYLGQIRDKVDITVLLIHEGVPARQSSFGATDVSRRLNKDIELAKQ
HCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCHHHHHHH
VQGLDIIITGHAHVGTPEPIKVNNTLIVSTDAYGIDLGKLVLNIDDKSKKITGYKGQLIN
CCCCEEEEECCCCCCCCCCEEECCEEEEECCCCCCEEEEEEEEECCCCCEECCCCCEEEE
IYAGDVEPDRATADKIALWTDKLKEITQQKIGKASAELTRSYGESSPVGNMVGDAFLAMD
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHEEEC
PRAQFAFVNSGGLRSELAQGEIKLGDIITLFPFPDDLSYMEINGKQLRSLMNHAANLTNG
CCCEEEEEECCCCHHHHHCCCEEECCEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHH
ILQVSKGIEMKYDSRKPLNQRIVSLTVQGQPLKDDAWYPIVVNSFLATGGDGFSAFTEGR
HHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCC
NIKTVAGSGAAEAVVDYIKSQAEISPNEQKRIKDVAL
CEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]