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The map label for this gene is puuB [H]

Identifier: 157368858

GI number: 157368858

Start: 679278

End: 680642

Strand: Direct

Name: puuB [H]

Synonym: Spro_0613

Alternate gene names: 157368858

Gene position: 679278-680642 (Clockwise)

Preceding gene: 157368857

Following gene: 157368859

Centisome position: 12.47

GC content: 56.48

Gene sequence:

>1365_bases
ATGGTGGCCGCCCGGCCGCTTGCCGATAATCCGATGACAGAGCAGGGCATCGGGAGTGCGGCAGTGAATAATAATATCGA
ATCCTTAACCTATTACGCGGCAACCAAAAAGTACGATCTGCGCTTCCCGACGCTGGAAGAAGATCTGGACGTTGACGTGG
TGATCATCGGCGGTGGTTTCTCCGGCATCAACACTGCGCTGGAACTGGCGGAACGGGGCATCACCAATATCGCTATTCTG
GAAGGCCGCCATTTGGGCTACGGCGGCACCGGACGCAACGGCGGGCAGGTGATGGCCGGCATCGGCCACGATCTGGAGAA
GATCAAACGTCACGTCGGCCCGGCCGGGTTGGAAACCATCTTCAAAATCAGCAATCTCGGCGCCGGGATCATTCGCGAAC
GCATCAAAAAATACCAAATCGATGCTGATTTCTGCTTTGGCTACGGCTACCTCGGCAGCAATGCGCGTCAGGAGAAAACC
CTGCGCAGTTGGCTGAAGGAGTTTAAGGCGGTCTCGCCGGATGAAGAGATCGAGCTCTATACCGGTGCCGAGGTGAAGCA
GGTGGTGGGATCGGACGCTTACACCTGCGCATTGAAACACATGGGCGGCGGCCACGTACATTCGCTCAATCTGCTACTGG
GCGAGGCCCAGGCGTTGAGTGGCTACGGCGTGAAGATCTTCGAAAACAGCAGTGTGATTGACGTGGAGTATGGCCAGCGC
ATAAAAGTGCGTACCGCCATGGGGTCAGTGCGTGCCAACAAAATGCTGTGGGCATGCAACGGTTTTCTCAACGGCATGGA
GCCGTTCATCTACAACAAAACCATTAATACCTATGCTTTCCAACTGGCGACCGAACCGCTGTCTGACGAGCTGATCCGCC
AGATCAGCCCGATCCGCGGGGCCTACAGCGATATCCGCCCGGTGATCGATTATTACCGGGTGACCAACGAAAACCGCCTG
CTGTTCGGTAGCGCTACCCGATTGATCGAATACATTCCGGCGGATCTCAAAGCCTGGAACCGCAACCTGATGCTGAAGGT
GTTCCCCTATTTGCACGACGTCAAAATTGACCTGGCCTGGGGCGGGCCGCTGTGCTGCAGCGCCAACCTTTTCCCACAGA
TCGGTACCTTGCCGCAGCACGATAACGTGTTCTACGTGCAGGGTTATTCCGGCTTTGGCGTGACCCCAAGCCATATCGTT
TGCAAGGTGTTGGCGGAGGGCATGAGTGAAGGATCCGATCGTTACGATCTGATGAGCTCGATCCCACACGTCAATATCTT
CGGCAAGGACAAACTACGGCGGGTGATGACTACCGCCGGCAAGGTGTGGCACCAGACTTCCGGCTACTGGAAAGGCCGCC
GTTAA

Upstream 100 bases:

>100_bases
TATTGGTTCGCAGGCCGAACTGTTCAGCCTGCTGCTGGCCACGCCGCTGCGTCGCAGCGCCTGAGTGTTAATTATTTGTT
GCGAATGTCCCTAAGGGGAC

Downstream 100 bases:

>100_bases
TTGTTACCTGAGGAGAGAGAAATGAAACCATTACTGCTTAAACAACCGCTGCCTGAACTGCTGGAAATTGGCAGCGTCAG
CAACCTGGGCGCCACCGTGA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MVAARPLADNPMTEQGIGSAAVNNNIESLTYYAATKKYDLRFPTLEEDLDVDVVIIGGGFSGINTALELAERGITNIAIL
EGRHLGYGGTGRNGGQVMAGIGHDLEKIKRHVGPAGLETIFKISNLGAGIIRERIKKYQIDADFCFGYGYLGSNARQEKT
LRSWLKEFKAVSPDEEIELYTGAEVKQVVGSDAYTCALKHMGGGHVHSLNLLLGEAQALSGYGVKIFENSSVIDVEYGQR
IKVRTAMGSVRANKMLWACNGFLNGMEPFIYNKTINTYAFQLATEPLSDELIRQISPIRGAYSDIRPVIDYYRVTNENRL
LFGSATRLIEYIPADLKAWNRNLMLKVFPYLHDVKIDLAWGGPLCCSANLFPQIGTLPQHDNVFYVQGYSGFGVTPSHIV
CKVLAEGMSEGSDRYDLMSSIPHVNIFGKDKLRRVMTTAGKVWHQTSGYWKGRR

Sequences:

>Translated_454_residues
MVAARPLADNPMTEQGIGSAAVNNNIESLTYYAATKKYDLRFPTLEEDLDVDVVIIGGGFSGINTALELAERGITNIAIL
EGRHLGYGGTGRNGGQVMAGIGHDLEKIKRHVGPAGLETIFKISNLGAGIIRERIKKYQIDADFCFGYGYLGSNARQEKT
LRSWLKEFKAVSPDEEIELYTGAEVKQVVGSDAYTCALKHMGGGHVHSLNLLLGEAQALSGYGVKIFENSSVIDVEYGQR
IKVRTAMGSVRANKMLWACNGFLNGMEPFIYNKTINTYAFQLATEPLSDELIRQISPIRGAYSDIRPVIDYYRVTNENRL
LFGSATRLIEYIPADLKAWNRNLMLKVFPYLHDVKIDLAWGGPLCCSANLFPQIGTLPQHDNVFYVQGYSGFGVTPSHIV
CKVLAEGMSEGSDRYDLMSSIPHVNIFGKDKLRRVMTTAGKVWHQTSGYWKGRR
>Mature_454_residues
MVAARPLADNPMTEQGIGSAAVNNNIESLTYYAATKKYDLRFPTLEEDLDVDVVIIGGGFSGINTALELAERGITNIAIL
EGRHLGYGGTGRNGGQVMAGIGHDLEKIKRHVGPAGLETIFKISNLGAGIIRERIKKYQIDADFCFGYGYLGSNARQEKT
LRSWLKEFKAVSPDEEIELYTGAEVKQVVGSDAYTCALKHMGGGHVHSLNLLLGEAQALSGYGVKIFENSSVIDVEYGQR
IKVRTAMGSVRANKMLWACNGFLNGMEPFIYNKTINTYAFQLATEPLSDELIRQISPIRGAYSDIRPVIDYYRVTNENRL
LFGSATRLIEYIPADLKAWNRNLMLKVFPYLHDVKIDLAWGGPLCCSANLFPQIGTLPQHDNVFYVQGYSGFGVTPSHIV
CKVLAEGMSEGSDRYDLMSSIPHVNIFGKDKLRRVMTTAGKVWHQTSGYWKGRR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=416, Percent_Identity=34.6153846153846, Blast_Score=250, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 50225; Mature: 50225

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAARPLADNPMTEQGIGSAAVNNNIESLTYYAATKKYDLRFPTLEEDLDVDVVIIGGGF
CCCCCCCCCCCCCCCCCCCHHHCCCHHHEEEEEECCEEECCCCCCCCCCCEEEEEECCCC
SGINTALELAERGITNIAILEGRHLGYGGTGRNGGQVMAGIGHDLEKIKRHVGPAGLETI
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHH
FKISNLGAGIIRERIKKYQIDADFCFGYGYLGSNARQEKTLRSWLKEFKAVSPDEEIELY
HHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
TGAEVKQVVGSDAYTCALKHMGGGHVHSLNLLLGEAQALSGYGVKIFENSSVIDVEYGQR
CCCHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCHHHCCCCCEEEECCCCEEEECCCCE
IKVRTAMGSVRANKMLWACNGFLNGMEPFIYNKTINTYAFQLATEPLSDELIRQISPIRG
EEEEEHHCCHHHCCEEEEECCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHC
AYSDIRPVIDYYRVTNENRLLFGSATRLIEYIPADLKAWNRNLMLKVFPYLHDVKIDLAW
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHCCCCEEEEEECCEEEEEEEEEE
GGPLCCSANLFPQIGTLPQHDNVFYVQGYSGFGVTPSHIVCKVLAEGMSEGSDRYDLMSS
CCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHC
IPHVNIFGKDKLRRVMTTAGKVWHQTSGYWKGRR
CCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVAARPLADNPMTEQGIGSAAVNNNIESLTYYAATKKYDLRFPTLEEDLDVDVVIIGGGF
CCCCCCCCCCCCCCCCCCCHHHCCCHHHEEEEEECCEEECCCCCCCCCCCEEEEEECCCC
SGINTALELAERGITNIAILEGRHLGYGGTGRNGGQVMAGIGHDLEKIKRHVGPAGLETI
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHH
FKISNLGAGIIRERIKKYQIDADFCFGYGYLGSNARQEKTLRSWLKEFKAVSPDEEIELY
HHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
TGAEVKQVVGSDAYTCALKHMGGGHVHSLNLLLGEAQALSGYGVKIFENSSVIDVEYGQR
CCCHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCHHHCCCCCEEEECCCCEEEECCCCE
IKVRTAMGSVRANKMLWACNGFLNGMEPFIYNKTINTYAFQLATEPLSDELIRQISPIRG
EEEEEHHCCHHHCCEEEEECCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHC
AYSDIRPVIDYYRVTNENRLLFGSATRLIEYIPADLKAWNRNLMLKVFPYLHDVKIDLAW
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHCCCCEEEEEECCEEEEEEEEEE
GGPLCCSANLFPQIGTLPQHDNVFYVQGYSGFGVTPSHIVCKVLAEGMSEGSDRYDLMSS
CCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHC
IPHVNIFGKDKLRRVMTTAGKVWHQTSGYWKGRR
CCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]