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The map label for this gene is dcuA [H]

Identifier: 157368652

GI number: 157368652

Start: 454263

End: 455564

Strand: Reverse

Name: dcuA [H]

Synonym: Spro_0405

Alternate gene names: 157368652

Gene position: 455564-454263 (Counterclockwise)

Preceding gene: 157368653

Following gene: 157368651

Centisome position: 8.36

GC content: 56.91

Gene sequence:

>1302_bases
ATGTTAGCCGTAGAATTGGTTATCGTTCTGCTGGCCATTTTTTTAGGGGCCAGGCTGGGCGGTATCGGAATTGGATTTGC
AGGAGGGCTGGGGGTGTTGGTCCTGGCGCTGATTGGCGTGAAGCCGGGCAGCATTCCGTTCGACGTGATATCCATCATCA
TGGCCGTCATTGCCGCGATCTCGGCAATGCAGGTCGCCGGTGGCATGGATTATCTGGTTCAGCAAACGGAAAAACTGCTG
CGCAAGAACCCTAAGCACATCACCATTCTGGCACCGATCGTCACCTATTTTCTGACCATCTTCGCCGGTACTGGCAACAT
TTCGCTTTCCGCTCTGCCGGTGATTGCAGAAGTGGCGAAAGAACAGGGCATCAAGCCTTGCCGCCCGCTGTCTACCGCCG
TGGTCTCCGCCCAGATTGCCATTACCGCCTCGCCAATTTCCGCAGCGGTAGTCTACATGTCCTCAGTAATGGAAGGTCAC
GGCGTCAGCTACCTGCACCTGCTGATGGTGGTGATGCCTTCGACCTTCTGCGCCGTGGTACTGATGTCGCTGCTGGTAAG
CTGGCTGTTTGACTCCAAACTGTCTGACGATCCGGTTTACCTGAAACGCCTTGAAGAAGGCCTGGTCACCCTGCGCGGCA
ACAAAGTGATGGAAATCAAACCGCGCGCCAAAGTGTCTGTGCTGCTGTTCCTGCTGGGCGTGCTGTGCGTGGTGGCCTAC
GCCATTATCAACAGTCCGGGTCTGGGCCTGGTGGCCAAACCCTTGATGAACACCACCAACGCGATTTTGATCATCATGCT
GAGCGTCGCCACCCTGACCACCTTACTGTGTAAAGTCGATACCGATATGGTACTCAACTCCAGCACCTTTAAGGCCGGCA
TGAGCGCCTGTATCTGTATTCTGGGCGTGGCCTGGCTGGGTGACACCTTTGTGCAGGCCAACATCGACTGGATCAAAGAT
ACCGCAGGCAGCGTGATCCAATCACACCCGTGGTTGTTGGCGGTGATTTTCTTCTTCTGCTCGGCGCTGCTGTACTCTCA
GGCCGCCACCGCCAAGGCGCTGATGCCAATGGCACTGGCGCTGAACGTCTCGCCATTGACCGCCGTCGCGTCCTTTGCCG
CCGTCTCCGGGCTGTTTATCCTGCCGACCTACCCTACGCTGGTCGCCGCAGTACAGATGGATGACACCGGCACCACACGT
ATCGGCCGCTTTGTGTTTAACCACCCGTTCTTTATTCCCGGCACCATCGGGGTGATATTGGCGGTGCTGTTCGGCTTCCT
GCTGGGCAGCGTCATGCTGTAA

Upstream 100 bases:

>100_bases
GCACGCTAAATCCTCTGGATAGCGTGCCTTTTTACTTTCCATCGCCCACAAAATTTTAACTTTTTATTTTCAAATAATCT
TATTTTAAGGAGTAGACACT

Downstream 100 bases:

>100_bases
TGCTTTCACCGGGGCTACGGCCCCGGTTTCCCCAACCCGCCCCACTGGCGATAAACTCGCCTTCTAGCCGACCGTCCTTC
AAGGTATAGTGCCCACTGAT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLAVELVIVLLAIFLGARLGGIGIGFAGGLGVLVLALIGVKPGSIPFDVISIIMAVIAAISAMQVAGGMDYLVQQTEKLL
RKNPKHITILAPIVTYFLTIFAGTGNISLSALPVIAEVAKEQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GVSYLHLLMVVMPSTFCAVVLMSLLVSWLFDSKLSDDPVYLKRLEEGLVTLRGNKVMEIKPRAKVSVLLFLLGVLCVVAY
AIINSPGLGLVAKPLMNTTNAILIIMLSVATLTTLLCKVDTDMVLNSSTFKAGMSACICILGVAWLGDTFVQANIDWIKD
TAGSVIQSHPWLLAVIFFFCSALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGRFVFNHPFFIPGTIGVILAVLFGFLLGSVML

Sequences:

>Translated_433_residues
MLAVELVIVLLAIFLGARLGGIGIGFAGGLGVLVLALIGVKPGSIPFDVISIIMAVIAAISAMQVAGGMDYLVQQTEKLL
RKNPKHITILAPIVTYFLTIFAGTGNISLSALPVIAEVAKEQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GVSYLHLLMVVMPSTFCAVVLMSLLVSWLFDSKLSDDPVYLKRLEEGLVTLRGNKVMEIKPRAKVSVLLFLLGVLCVVAY
AIINSPGLGLVAKPLMNTTNAILIIMLSVATLTTLLCKVDTDMVLNSSTFKAGMSACICILGVAWLGDTFVQANIDWIKD
TAGSVIQSHPWLLAVIFFFCSALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGRFVFNHPFFIPGTIGVILAVLFGFLLGSVML
>Mature_433_residues
MLAVELVIVLLAIFLGARLGGIGIGFAGGLGVLVLALIGVKPGSIPFDVISIIMAVIAAISAMQVAGGMDYLVQQTEKLL
RKNPKHITILAPIVTYFLTIFAGTGNISLSALPVIAEVAKEQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GVSYLHLLMVVMPSTFCAVVLMSLLVSWLFDSKLSDDPVYLKRLEEGLVTLRGNKVMEIKPRAKVSVLLFLLGVLCVVAY
AIINSPGLGLVAKPLMNTTNAILIIMLSVATLTTLLCKVDTDMVLNSSTFKAGMSACICILGVAWLGDTFVQANIDWIKD
TAGSVIQSHPWLLAVIFFFCSALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGRFVFNHPFFIPGTIGVILAVLFGFLLGSVML

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=433, Percent_Identity=87.7598152424942, Blast_Score=723, Evalue=0.0,
Organism=Escherichia coli, GI1790565, Length=405, Percent_Identity=35.8024691358025, Blast_Score=238, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45676; Mature: 45676

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAVELVIVLLAIFLGARLGGIGIGFAGGLGVLVLALIGVKPGSIPFDVISIIMAVIAAI
CHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SAMQVAGGMDYLVQQTEKLLRKNPKHITILAPIVTYFLTIFAGTGNISLSALPVIAEVAK
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGHGVSYLHLLMVVMPSTFCAVV
HCCCCCCCCHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LMSLLVSWLFDSKLSDDPVYLKRLEEGLVTLRGNKVMEIKPRAKVSVLLFLLGVLCVVAY
HHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
AIINSPGLGLVAKPLMNTTNAILIIMLSVATLTTLLCKVDTDMVLNSSTFKAGMSACICI
HHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHH
LGVAWLGDTFVQANIDWIKDTAGSVIQSHPWLLAVIFFFCSALLYSQAATAKALMPMALA
HHHHHHCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTRIGRFVFNHPFFIPGTIGVIL
CCCCHHHHHHHHHHHCCEEEECCCHHHHEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHH
AVLFGFLLGSVML
HHHHHHHHHHHCC
>Mature Secondary Structure
MLAVELVIVLLAIFLGARLGGIGIGFAGGLGVLVLALIGVKPGSIPFDVISIIMAVIAAI
CHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SAMQVAGGMDYLVQQTEKLLRKNPKHITILAPIVTYFLTIFAGTGNISLSALPVIAEVAK
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGHGVSYLHLLMVVMPSTFCAVV
HCCCCCCCCHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LMSLLVSWLFDSKLSDDPVYLKRLEEGLVTLRGNKVMEIKPRAKVSVLLFLLGVLCVVAY
HHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
AIINSPGLGLVAKPLMNTTNAILIIMLSVATLTTLLCKVDTDMVLNSSTFKAGMSACICI
HHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHH
LGVAWLGDTFVQANIDWIKDTAGSVIQSHPWLLAVIFFFCSALLYSQAATAKALMPMALA
HHHHHHCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTRIGRFVFNHPFFIPGTIGVIL
CCCCHHHHHHHHHHHCCEEEECCCHHHHEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHH
AVLFGFLLGSVML
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]