Click here to switch to the map view.

The map label for this gene is rffA [H]

Identifier: 157368417

GI number: 157368417

Start: 189488

End: 190618

Strand: Direct

Name: rffA [H]

Synonym: Spro_0168

Alternate gene names: 157368417

Gene position: 189488-190618 (Clockwise)

Preceding gene: 157368416

Following gene: 157368418

Centisome position: 3.48

GC content: 55.53

Gene sequence:

>1131_bases
ATGATCCCATTTAACGCACCACCGGTTGTTGGCACTGAACTTGACTATATGCAGGCTGCGATGGGCAGTGGCAAGCTGTG
TGGTGACGGTGGCTTTACCCGTCGTTGCCAGCAGTGGATGGAACAGCGTTTTGGCAGCCCGAAGGTGCTGTTGACGCCGT
CCTGCACTGCCTCGCTGGAGATGGCGGCGATCCTGCTGGATATTCAGCCGGGCGATGAAGTGATCATGCCGAGCTTCACC
TTTGTTTCAACCGCCAACGCCTTCGTGCTGCGTGGTGCCACCGTGGTGTTTGTCGACCTGCGCCCTGACACCATGAACAT
CGATGAGAGCAAAATCGAAGCGGCAATCACCGATAAGACCCGTGCCATTGTACCGGTGCATTACGCCGGTGTAGCCTGCG
AGATGGACACCATCATGGCGCTGGCGGAAAAATACAAATTGTATGTAGTTGAAGATGCGGCACAGGGCGTGATGTCCACC
TACAAGGGGAAAGCGCTGGGTACCATCGGCCATATTGGCTGCTTTAGCTTTCACGAAACCAAAAACTACACCGCAGGCGG
TGAGGGCGGCGCTACGCTGATTAACGATTCGGCGCTGATTGATCGGGCGGAAGTCATTCGTGAGAAAGGCACCAACCGCA
GCCAGTTCTTCCGTGGGCAGGTGGATAAGTACACCTGGCGTGATATCGGCTCCAGCTATCTGATGTCAGATCTGCAGGCC
GCGTATCTGTGGGGCCAACTGGAAGCGGCTGACCGCATCAACCAACGCCGTCTGGCGCTGTGGCAGAAGTATTACGACAG
TTTCCTGCCGTTAGCCGAGGCGGGCCGTATCGTGCTACCGAGCATCCCGGCAGACTGCGTGCAAAATGCGCATATGTTCT
ATATCAAACTGCGCGATGTTGAAGACCGTACGGCGTTTATCGATTATATGAAAGAAGCCGAGATCATGACGGTATTCCAT
TACATCCCACTGCATGATTGCCCGGCGGGCGATCGCTTTGGCCGCTTTGCCGGGGAAGATCGCTTCACCAGCCAGGAGAG
CGCGCGGTTGGTACGTTTGCCGCTGTTCTACAATATGACTGATGTCACTCAGCGCACGGTGATCAATACCATTCTCAACT
TCTTCGCCTGA

Upstream 100 bases:

>100_bases
CGGGTTGCGACCCAGTTCGGTAACATCGCCGCACTACGGCTCTATCAACGACAGGGTGCTGCTATTGAAAGCACCTCGTA
TTGGTTATACAGAGGAAGAC

Downstream 100 bases:

>100_bases
TATGTCGCTGGCAAAAGCATCGATTTGGACCGCCGGCTCTACGCTTATCAAGATTGGCGTGGGGCTGTTGGTGGTGAAAC
TGCTGGCGGTGGCCTTTGGC

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIPFNAPPVVGTELDYMQAAMGSGKLCGDGGFTRRCQQWMEQRFGSPKVLLTPSCTASLEMAAILLDIQPGDEVIMPSFT
FVSTANAFVLRGATVVFVDLRPDTMNIDESKIEAAITDKTRAIVPVHYAGVACEMDTIMALAEKYKLYVVEDAAQGVMST
YKGKALGTIGHIGCFSFHETKNYTAGGEGGATLINDSALIDRAEVIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWGQLEAADRINQRRLALWQKYYDSFLPLAEAGRIVLPSIPADCVQNAHMFYIKLRDVEDRTAFIDYMKEAEIMTVFH
YIPLHDCPAGDRFGRFAGEDRFTSQESARLVRLPLFYNMTDVTQRTVINTILNFFA

Sequences:

>Translated_376_residues
MIPFNAPPVVGTELDYMQAAMGSGKLCGDGGFTRRCQQWMEQRFGSPKVLLTPSCTASLEMAAILLDIQPGDEVIMPSFT
FVSTANAFVLRGATVVFVDLRPDTMNIDESKIEAAITDKTRAIVPVHYAGVACEMDTIMALAEKYKLYVVEDAAQGVMST
YKGKALGTIGHIGCFSFHETKNYTAGGEGGATLINDSALIDRAEVIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWGQLEAADRINQRRLALWQKYYDSFLPLAEAGRIVLPSIPADCVQNAHMFYIKLRDVEDRTAFIDYMKEAEIMTVFH
YIPLHDCPAGDRFGRFAGEDRFTSQESARLVRLPLFYNMTDVTQRTVINTILNFFA
>Mature_376_residues
MIPFNAPPVVGTELDYMQAAMGSGKLCGDGGFTRRCQQWMEQRFGSPKVLLTPSCTASLEMAAILLDIQPGDEVIMPSFT
FVSTANAFVLRGATVVFVDLRPDTMNIDESKIEAAITDKTRAIVPVHYAGVACEMDTIMALAEKYKLYVVEDAAQGVMST
YKGKALGTIGHIGCFSFHETKNYTAGGEGGATLINDSALIDRAEVIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWGQLEAADRINQRRLALWQKYYDSFLPLAEAGRIVLPSIPADCVQNAHMFYIKLRDVEDRTAFIDYMKEAEIMTVFH
YIPLHDCPAGDRFGRFAGEDRFTSQESARLVRLPLFYNMTDVTQRTVINTILNFFA

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=376, Percent_Identity=85.3723404255319, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI145693159, Length=382, Percent_Identity=32.4607329842932, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42008; Mature: 42008

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFNAPPVVGTELDYMQAAMGSGKLCGDGGFTRRCQQWMEQRFGSPKVLLTPSCTASLE
CCCCCCCCCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHE
MAAILLDIQPGDEVIMPSFTFVSTANAFVLRGATVVFVDLRPDTMNIDESKIEAAITDKT
EEEEEEEECCCCCEECCCCHHHHCCCEEEEECCEEEEEEECCCCCCCCHHHHHEEECCCC
RAIVPVHYAGVACEMDTIMALAEKYKLYVVEDAAQGVMSTYKGKALGTIGHIGCFSFHET
EEEEEEEECCEEEHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCCCCCCHHHHEEEEEECC
KNYTAGGEGGATLINDSALIDRAEVIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHH
AYLWGQLEAADRINQRRLALWQKYYDSFLPLAEAGRIVLPSIPADCVQNAHMFYIKLRDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCCCHHHHCCCEEEEEEEECC
EDRTAFIDYMKEAEIMTVFHYIPLHDCPAGDRFGRFAGEDRFTSQESARLVRLPLFYNMT
CHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECHH
DVTQRTVINTILNFFA
HHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIPFNAPPVVGTELDYMQAAMGSGKLCGDGGFTRRCQQWMEQRFGSPKVLLTPSCTASLE
CCCCCCCCCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHE
MAAILLDIQPGDEVIMPSFTFVSTANAFVLRGATVVFVDLRPDTMNIDESKIEAAITDKT
EEEEEEEECCCCCEECCCCHHHHCCCEEEEECCEEEEEEECCCCCCCCHHHHHEEECCCC
RAIVPVHYAGVACEMDTIMALAEKYKLYVVEDAAQGVMSTYKGKALGTIGHIGCFSFHET
EEEEEEEECCEEEHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCCCCCCHHHHEEEEEECC
KNYTAGGEGGATLINDSALIDRAEVIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHH
AYLWGQLEAADRINQRRLALWQKYYDSFLPLAEAGRIVLPSIPADCVQNAHMFYIKLRDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCCCHHHHCCCEEEEEEEECC
EDRTAFIDYMKEAEIMTVFHYIPLHDCPAGDRFGRFAGEDRFTSQESARLVRLPLFYNMT
CHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECHH
DVTQRTVINTILNFFA
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]